Response of Various Tomato Genotypes to Begomovirus Infection and Its Improved Diagnostic

Biological and molecular characterization of bean bushy stunt virus, a novel bipartite begomovirus infecting common bean in northwestern Argentina

“…However, false negatives can also occur, as we unexpectedly observed with three samples corresponding to BGMV isolates that could not be detected using the specific probe, probably due to the poor quality of the extracted DNA or a low viral titer [1]. On the other hand, only 21.2% of the symptomatic common bean samples were infected with begomoviruses; therefore, the remaining samples could have been infected by other viruses [1,19] or affected by herbicides [21]. In this work, we detected and characterized BBSV, a new begomovirus infecting common bean crops in Argentina.…”

“…The use of specific detection probes can help us to demonstrate the presence of previously reported begomoviruses or to provide clues regarding the presence of still unknown viruses. However, false negatives can also occur, as we unexpectedly observed with three samples corresponding to BGMV isolates that could not be detected using the specific probe, probably due to the poor quality of the extracted DNA or a low viral titer [1]. On the other hand, only 21.2% of the symptomatic common bean samples were infected with begomoviruses; therefore, the remaining samples could have been infected by other viruses [1,19] or affected by herbicides [21].…”