Response of various target genes to diet-delivered dsRNA mediated RNA interference in the cotton bollworm, Helicoverpa armigera

Asokan, R.; Chandra, G. Sharath; Manamohan, M.; Kumar, N. K. Krishna; Sita, Timothy L.

doi:10.1007/s10340-013-0541-7

Cited by 41 publications

(38 citation statements)

References 36 publications

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“…Similar results have been reported by Asokan et al. (). They evaluated effect of two concentrations of dsRNA (10 and 20 μg) in Helicoverpa armigera trypsin transcript abundant.…”

Section: Discussionsupporting

confidence: 92%

Molecular Identification of Cysteine and Trypsin Protease, Effect of Different Hosts on Protease Expression, and Rnai Mediated Silencing of Cysteine Protease Gene in the Sunn Pest

Amiri

Bandani

2015

Arch Insect Biochem Physiol

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Sunn pest, Eurygaster integriceps, is a serious pest of cereals in the wide area of the globe from Near and Middle East to East and South Europe and North Africa. This study described for the first time, identification of E. integriceps trypsin serine protease and cathepsin-L cysteine, transcripts involved in digestion, which might serve as targets for pest control management. A total of 478 and 500 base pair long putative trypsin and cysteine gene sequences were characterized and named Tryp and Cys, respectively. In addition, the tissue-specific relative gene expression levels of these genes as well as gluten hydrolase (Gl) were determined under different host kernels feeding conditions. Result showed that mRNA expression of Cys, Tryp, and Gl was significantly affected after feeding on various host plant species. Transcript levels of these genes were most abundant in the wheat-fed E. integriceps larvae compared to other hosts. The Cys transcript was detected exclusively in the gut, whereas the Gl and Tryp transcripts were detectable in both salivary glands and gut. Also possibility of Sunn pest gene silencing was studied by topical application of cysteine double-stranded RNA (dsRNA). The results indicated that topically applied dsRNA on fifth nymphal stage can penetrate the cuticle of the insect and induce RNA interference. The Cys gene mRNA transcript in the gut was reduced to 83.8% 2 days posttreatment. Also, it was found that dsRNA of Cys gene affected fifth nymphal stage development suggesting the involvement of this protease in the insect growth, development, and molting.

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“…Similar results have been reported by Asokan et al. (). They evaluated effect of two concentrations of dsRNA (10 and 20 μg) in Helicoverpa armigera trypsin transcript abundant.…”

Section: Discussionsupporting

confidence: 92%

Molecular Identification of Cysteine and Trypsin Protease, Effect of Different Hosts on Protease Expression, and Rnai Mediated Silencing of Cysteine Protease Gene in the Sunn Pest

Amiri

Bandani

2015

Arch Insect Biochem Physiol

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“…Important aspects to be taken into account include: (i) Encoding of essential processes, as this dictates the phenotypic effects in the case of successful systemic silencing of transcript. Previous studies on ingestion-mediated RNAi knockdown in H. armigera have included genes such as JHAMT (Asokan et al, 2014(Asokan et al, , 2013, ecdysone receptor and ultraspiracle protein (involved in insect molting and metamorphosis) (Yang and Han, 2014a;Zhu et al, 2012), chitin synthase (synthesis of chitin necessary for trachea, cuticle and midgut development) (Jin et al, 2015) and molt-regulating transcription factor (regulation of metamorphosis-related gene expression) (Xiong, 2013).…”

Section: Target Genementioning

confidence: 99%

“…Genes with lower transcript abundance will require less dsRNA for silencing, e.g. JHAMT transcript levels detected after 32-35 cycles compared to 16-25 for other target genes tested (Asokan et al, 2014 (Asokan et al, 2013).…”

Section: Target Genementioning

confidence: 99%

Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

Lim

Robinson

Jain

et al. 2016

Journal of Insect Physiology

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“…Asokan et al. () targeted five unrelated mRNAs in Helicoverpa armigera (Hübner) and observed variation in silencing efficiency from 21 to 95%.…”

Section: Implementation Of Ernai In Pest Speciesmentioning

confidence: 99%

Implementing the sterile insect technique with RNA interference – a review

Darrington

Dalmay

Morrison

et al. 2017

Entomologia Exp Applicata

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We review RNA interference (RNAi) of insect pests and its potential for implementing sterile insect technique (SIT)‐related control. The molecular mechanisms that support RNAi in pest species are reviewed in detail, drawing on literature from a range of species including Drosophila melanogaster Meigen and Homo sapiens L. The underlying genes that enable RNAi are generally conserved across taxa, although variance exists in both their form and function. RNAi represents a plausible, non‐GM system for targeting populations of insects for control purposes, if RNAi effector molecules can be delivered environmentally (eRNAi). We consider studies of eRNAi from across several insect orders and review to what extent taxonomy, genetics, and differing methods of double‐stranded (ds) RNA synthesis and delivery can influence the efficiency of gene knockdown. Several factors, including the secondary structure of the target mRNA and the specific nucleotide sequence of dsRNA effector molecules, can affect the potency of eRNAi. However, taxonomic relationships between insects cannot be used to reliably forecast the efficiency of an eRNAi response. The mechanisms by which insects acquire dsRNA from their environment require further research, but the evidence to date suggests that endocytosis and transport channels both play key roles. Delivery of RNA molecules packaged in intermediary carriers such as bacteria or nanoparticles may facilitate their entry into and through the gut, and enable the evasion of host defence systems, such as toxic pH, that would otherwise attenuate the potential for RNAi.

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Response of various target genes to diet-delivered dsRNA mediated RNA interference in the cotton bollworm, Helicoverpa armigera

Cited by 41 publications

References 36 publications

Molecular Identification of Cysteine and Trypsin Protease, Effect of Different Hosts on Protease Expression, and Rnai Mediated Silencing of Cysteine Protease Gene in the Sunn Pest

Molecular Identification of Cysteine and Trypsin Protease, Effect of Different Hosts on Protease Expression, and Rnai Mediated Silencing of Cysteine Protease Gene in the Sunn Pest

Diet-delivered RNAi in Helicoverpa armigera – Progresses and challenges

Implementing the sterile insect technique with RNA interference – a review

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