Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the Northeast region of Brazil

Araújo, Antônio Edilson da Silva; Baldani, V. L. D.; Galisa, Péricles de Souza; Pereira, J. A.; Baldani, José Ivo

doi:10.1016/j.apsoil.2012.10.004

Cited by 53 publications

(21 citation statements)

References 34 publications

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“…Indolic compounds production by isolates was similar to those previously reported for rice plants isolates, an average production of 1.8 and 53 µg IAA ml -1 (Kuss et al, 2007;Rodrigues et al, 2008;Araújo et al, 2013). After 48 h, amount of indole accumulation was lesser than after 72 h probably due to degradation of indole compounds as reported by Leveau and Gerards (2008).…”

Section: Production Of Indolic Compoundssupporting

confidence: 89%

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Evaluation of diazotrophic bacteria from rice varieties (Oryza sativa L.) grown in Rio de Janeiro State, Brazil

Silva¹,

Amaral²,

Teixeira³

et al. 2018

Afr. J. Agric. Res.

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The indiscriminate application of nitrogen fertilizer in order to increase rice crop productivity contributes to environmental degradation. One of the possible alternatives to ease up this problem is the use of sustainable and cost-effective technologies such as biofertilizers to promote plant growth. This work aimed to isolate, characterize and select diazotrophic bacteria associated with rice cultivars EPAGRI-109 and BRS TROPICAL. About 39 bacteria belonging to the genera Azospirillum, Herbaspirillum, Paenibacillus, Ideonella and Pseudomonas were isolated. Based on the best results of acetylene reduction assay (ARA), indolic compounds production and phosphate solubilization, the isolates were selected as potential plant growth promoters which were tested on rice cultivars IR 42, EPAGRI 109 and BRS TROPICAL under axenic condition and the most efficient isolates promoted up to 31% of dry weight of the evaluated rice cultivars. Two promising isolates were selected in this work (Paenibacillus species strain EP3-16N and Nitrospirillum species strain EP4-1L), however, more studies are necessary to confirm this capacity in field conditions.

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Section: Production Of Indolic Compoundssupporting

confidence: 89%

“…Many genera of diazotrophs such as Azospirillum, Herbaspirillum, Burkhoderia and Sphingomonas have been isolated from rice and have shown positive effects on many rice varieties (Baldani et al, 2000;Tran Van et al, 2000;Baldani and Baldani, 2005;Rodrigues et al, 2006;Rodrigues et al, 2008;Pedraza et al, 2009;Araújo et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of diazotrophic bacteria from rice varieties (Oryza sativa L.) grown in Rio de Janeiro State, Brazil

Silva¹,

Amaral²,

Teixeira³

et al. 2018

Afr. J. Agric. Res.

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“…Peng et al (2002), however, reported that rhizobial inoculation known for their symbiotic relationship with legumes, could also increaserice grain yield, but little is known about the mechanism(s) involved. The beneficial effects of the selected rhizobialisolates could be due to their plant growth-promoting abilities namely biological N 2 fixation, phosphate solubilization and plant growthregulator/phytohormone and siderophore production, similar to the known beneficial effects of PGPR (Boddey et al, 1997;Verma et al, 2001;Araujo et al, 2013;Kloepper et al, 1980). Yanni et al (1997) have also shown beneficial interactions of rhizobial isolateson growth of rice, which was believed to be due to increased root efficiency in water and nutrient uptake.…”

Section: Introductionmentioning

confidence: 93%

Isolation and Characterization of Rhizobia and Plant Growth-Promoting Rhizobacteria and Their Effects on Growth of Rice Seedlings

Tan

Othman

Halimi

et al. 2014

American Journal of Agricultural and Biological Sciences

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Biofertilizer is a relatively safer, environmentally friendly and cost-effective approach as an alternative to reduce chemical fertilizer usage. The selection of bacterial strains with multiple beneficial characteristics are important to maximize the effectiveness on the host plant. Due to aforementioned interest, several Plant Growth-Promoting Rhizobacterial (PGPR) and rhizobial strains were isolated from rice and legume roots, respectively, at four locations in Malaysia namely Universiti Putra Malaysia (UPM), Serdang, Selangor; Besut, Terengganu; Tunjung, Kelantan and Sik, Kedah. Bacterial isolations were undertaken to select the best isolates which exhibit multiple beneficial effects to the rice plant and a total of 205 bacterial strains were isolated and categorized as follows; 94 rhizospheric and 107 endophytic bacteria from rice roots, one rhizobial strain from soybean and three from Mimosa pudica. These isolates were screened for their abilities to fix N 2 and solubilize phosphate; 52 were positive for both tests. The selected isolates were then tested for IAA production and other biochemical tests such as potassium solubilization, hydrolyzing enzymes (cellulase and pectinase) and iron siderophore productions. Four isolates, namely UPMB19 (rhizospheric PGPR from Tunjung, Kelantan), UPMB20 (endophytic PGPR from Besut, Terengganu), UPMR30 (rhizobia from soybean) and UPMR31 (rhizobia from Mimosa) were selected for subsequent plant inoculation tests with UPMB10, a PGPR isolated from oil palm root, as the reference strain. Based on 16S rDNA gene sequencing, these bacterial strains were identified under several genera: Lysinibacillus, Alcaligenes, Bradyrhizobium, Rhizobium and Bacillus, respectively. Results of plant inoculation test indicated that UPMB19 significantly enhanced the seedling height at the early growth stage (7 days after transplanting, DAT) which could be attributed to the higher N 2 fixation rate of this strain as compared to the other strains, except UPMB20. UPMB10 and UPMR31 also showed significant effects with increased seedling height and Total Dry Weight (TDW) at the later stage (14 DAT) possibly due to the higher bacterial population and IAA produced as observed in the residual nutrient solution without addition of fertilizer-N. This study has successfully demonstrated the effectiveness of locally isolated PGPR and rhizobial strains with multiple beneficial characteristics on early growth and vigor of rice seedlings under controlled condition. The result proved to be a vital information in the development of a liquid biofertilizer for rice; thus further studies will be done to evaluate the effectiveness of these isolates under glasshouse and field conditions on growth, tillering and yield of rice.

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“…Thus, in many cases, it is not possible to quantitatively associate the failure or success of plant growth promotion achieved with the inoculated bacterial population at a strain-specific resolution, leaving the outcome of the inoculation unexplained (17). Furthermore, the crop response to inoculation under field conditions heavily depends on the combination of the plant genotype and the bacterial strain (18)(19)(20), stressing the need for methodologies to evaluate the success of plant colonization accurately at a high resolution. Previously, we used whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) …”

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confidence: 99%

Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

Stets

Alquéres

Souza

et al. 2015

Appl Environ Microbiol

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bAzospirillum is a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide. Azospirillum brasilense strains exhibit a growth-promoting effect by means of phytohormone production and possibly by N 2 fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification of A. brasilense FP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences of A. brasilense FP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for the A. brasilense FP2 strain. These primer pairs were used to monitor quantitatively the population of A. brasilense in wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed that A. brasilense FP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (ϳ10 7 CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.A zospirillum is one of the most important genera of plant growth-promoting rhizobacteria found worldwide under a variety of environmental and soil conditions (1). The diazotroph Azospirillum brasilense is the best-studied species of the genus, is found in close association with many agriculturally important crops, and exerts beneficial effects on plant growth and productivity (2-4). Nitrogen fixation (5, 6) and the production of the auxin 3-indoleacetic acid (IAA) by many representatives of the genus Azospirillum are related to the growth promotion effects observed in inoculated plants, such as increases in root length and the numbers of root hairs and lateral roots (3).The biotechnological use of A. brasilense inoculants in Latin American and in Brazil, in particular, has increased in recent years (7). Strain FP2 is a spontaneous mutant of A. brasilense Sp7 (8). Strain Sp7 has been shown to be capable of stimulating the growth of several members of the family Poaceae and increasing the productivities of wheat and maize crops (2). Strain FP2 can also promote the growth of wheat (9) and enhance maize and wheat productivity under field conditions (unpublished data). Most of the A. brasilense inoculants in B...

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Response of traditional upland rice varieties to inoculation with selected diazotrophic bacteria isolated from rice cropped at the Northeast region of Brazil

Cited by 53 publications

References 34 publications

Evaluation of diazotrophic bacteria from rice varieties (Oryza sativa L.) grown in Rio de Janeiro State, Brazil

Evaluation of diazotrophic bacteria from rice varieties (Oryza sativa L.) grown in Rio de Janeiro State, Brazil

Isolation and Characterization of Rhizobia and Plant Growth-Promoting Rhizobacteria and Their Effects on Growth of Rice Seedlings

Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR

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