Response of Thymus lotocephalus In Vitro Cultures to Drought Stress and Role of Green Extracts in Cosmetics

Mansinhos, Inês; Gonçalves, Sandra; Rodríguez‐Solana, Raquel; Duarte, Hugo; Ordóñez-Díaz, José Luis; Moreno-Rojas, José Manuel; Romano, Anabela

doi:10.3390/antiox11081475

Cited by 9 publications

(9 citation statements)

References 91 publications

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“…Contrary to our results, severe drought stress did not affect the accumulation of rosmarinic acid in Salvia milltiorrhiza [44]. Furthermore, PEG showed negative effects on RA accumulation in Thymus lotocephalus shoots [56]. In summary, dehydration stress appears to modulate RA biosynthesis differently in related Lamiaceae species contingent upon the severity and duration of experimental conditions.…”

Section: Content Of Phenolic Acidscontrasting

confidence: 99%

Polyethylene Glycol (PEG)-Induced Dehydration Alters Enzymatic and Non-Enzymatic Components of the Antioxidant Defense System in Nepeta nervosa Royle ex Bentham

Nestorović Živković,

Aničić,

Matekalo

et al. 2023

Horticulturae

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Plants have developed a variety of defense mechanisms that allow them to recognize and respond to specific stressors by triggering complex signaling networks that cause appropriate biochemical changes to overcome the stress. In this study, we subjected in vitro grown N. nervosa plants to PEG-induced dehydration stress for 1 day (1DPT), 3 days (3DPT) and 6 days (6DPT). Our study investigated antioxidant enzyme activities, including catalase (CAT), peroxidase (POX) and superoxide dismutase (SOD), unveiling dynamic responses to PEG-induced water stress. CAT levels increased initially (1DPT) but declined with prolonged treatment; while POX activity significantly increased at 3DPT and 6DPT; and SOD, particularly the Mn-SOD3 isoform, demonstrated a substantial increase, emphasizing its role in the enzymatic free-radical scavenging activity. Furthermore, examination of the phenolic acid content revealed that rosmarinic acid (RA) was the predominant phenolic compound, followed by chlorogenic acid (CHLA), while ferulic acid (FA) and caffeic acid (CAFFA) were present in lower concentrations. Notably, PEG-induced dehydration significantly boosted RA content in N. nervosa plants at 3DPT. This increase highlights the plant’s response to oxidative stress conditions and its role in non-enzymatic antioxidant defense mechanisms. These findings significantly contribute to our comprehension of N. nervosa’s adaptive strategies under PEG-induced dehydration stress, offering valuable insights into plant stress physiology within industrial and agricultural contexts.

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Section: Content Of Phenolic Acidscontrasting

confidence: 99%

Polyethylene Glycol (PEG)-Induced Dehydration Alters Enzymatic and Non-Enzymatic Components of the Antioxidant Defense System in Nepeta nervosa Royle ex Bentham

Nestorović Živković,

Aničić,

Matekalo

et al. 2023

Horticulturae

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“…The antioxidant, anticancer, and antimicrobial properties of this class of phenolic compounds were also clearly demonstrated, their scavenging activity against different free radicals being 3−fold higher compared to industrial synthetic antioxidants. Additionally, in a study about the role of elicitation in alleviating drought stress in Thymus lotocephalus in vitro cultures, [ 35 ] found that MeJA was more effective than SA in promoting the biosynthesis of phenolic compounds (mainly phenolic acids), increasing their accumulation by up to 38.8%.…”

Section: Discussionmentioning

confidence: 99%

Impact of Elicitation on Plant Antioxidants Production in Taxus Cell Cultures

et al. 2023

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Elicited cell cultures of Taxus spp. are successfully used as sustainable biotechnological production systems of the anticancer drug paclitaxel, but the effect of the induced metabolomic changes on the synthesis of other bioactive compounds by elicitation has been scarcely studied. In this work, a powerful combinatorial approach based on elicitation and untargeted metabolomics was applied to unravel and characterize the effects of the elicitors 1 µM of coronatine (COR) or 150 µM of salicylic acid (SA) on phenolic biosynthesis in Taxus baccata cell suspensions. Differential effects on cell growth and the phenylpropanoid biosynthetic pathway were observed. Untargeted metabolomics analysis revealed a total of 83 phenolic compounds, mainly flavonoids, phenolic acids, lignans, and stilbenes. The application of multivariate statistics identified the metabolite markers attributed to elicitation over time: up to 34 compounds at 8 days, 41 for 16 days, and 36 after 24 days of culture. The most notable metabolic changes in phenolic metabolism occurred after 8 days of COR and 16 days of SA elicitation. Besides demonstrating the significant and differential impact of elicitation treatments on the metabolic fingerprint of T. baccata cell suspensions, the results indicate that Taxus ssp. biofactories may potentially supply not only taxanes but also valuable phenolic antioxidants, in an efficient optimization of resources.

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“…Roots regulate water and nutrient uptake and are the first to sense water deficits ( Hein et al., 2016 ). When plants experience drought conditions, a decrease in water transport from the roots to the aboveground parts through the xylem directly signals drought stress to the plant ( Mansinhos et al., 2022 ). Roots can be traced to coordinated cell division, elongation, and differentiation events to enhance their ability to absorb water ( Rellan-Alvarez et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Co-expression network analysis reveals PbTGA4 and PbAPRR2 as core transcription factors of drought response in an important timber species Phoebe bournei

Yu,

Yin,

Liu

et al. 2024

Front. Plant Sci.

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Phoebe bournei is one of the main afforestation tree species in subtropical regions of China and is famous for its timber. Its distribution and growth are significantly impaired by water conditions. Thus, it is essential to understand the mechanism of the stress response in P. bournei. Here, we analyzed the phenotypic changes and transcriptomic rearrangement in the leaves and roots of P. bournei seedlings grown for 0 h, 1 h, 24 h, and 72 h under simulated drought conditions (10% PEG 6000). The results showed that drought stress inhibited plant photosynthesis and increased oxidoreductase activity and abscisic acid (ABA) accumulation. Spatio-temporal transcriptomic analysis identified 2836 and 3704 differentially expressed genes (DEGs) in leaves and roots, respectively. The responsive genes in different organs presented various expression profiles at different times. Gene co-expression network analysis identified two core transcription factors, TGA4 and APRR2, from two modules that showed a strong positive correlation with ABA accumulation. Our study investigated the different responses of aboveground and belowground organs of P. bournei to drought stress and provides critical information for improving the drought resistance of this timber species.

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Response of Thymus lotocephalus In Vitro Cultures to Drought Stress and Role of Green Extracts in Cosmetics

Cited by 9 publications

References 91 publications

Polyethylene Glycol (PEG)-Induced Dehydration Alters Enzymatic and Non-Enzymatic Components of the Antioxidant Defense System in Nepeta nervosa Royle ex Bentham

Polyethylene Glycol (PEG)-Induced Dehydration Alters Enzymatic and Non-Enzymatic Components of the Antioxidant Defense System in Nepeta nervosa Royle ex Bentham

Impact of Elicitation on Plant Antioxidants Production in Taxus Cell Cultures

Co-expression network analysis reveals PbTGA4 and PbAPRR2 as core transcription factors of drought response in an important timber species Phoebe bournei

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