Response of the seagrass Posidonia oceanica to different light environments: Insights from a combined molecular and photo-physiological study

Dattolo, Emanuela; Ruocco, Michelina; Brunet, Christophe; Lorenti, Maurizio; Lauritano, Chiara; D’Esposito, Daniela; Luca, Pasquale De; Sanges, Remo; Mazzuca, Silvia; Procaccini, Gabriele

doi:10.1016/j.marenvres.2014.07.010

Cited by 85 publications

(76 citation statements)

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“…Only a limited number of functional studies has been conducted on P. oceanica , mostly looking at the expression of selected target genes e.g., refs 7–10. A first set of annotated genes was developed for P. oceanica , reconstructed from samples collected at different depths 10 , and a SSH (Suppression Subtractive Hybridization) library was built in order to detect differentially expressed genes 7,8,11 . These resources constitute an important first step towards the characterization of the transcriptome of the species, but the limited number of sequences is not at all representative of the whole transcriptome of the species.…”

Section: Background and Summarymentioning

confidence: 99%

Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

et al. 2016

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Posidonia oceanica is an endemic seagrass in the Mediterranean Sea, where it provides important ecosystem services and sustains a rich and diverse ecosystem. P. oceanica meadows extend from the surface to 40 meters depth. With the aim of boosting research in this iconic species, we generated a comprehensive RNA-Seq data set for P. oceanica by sequencing specimens collected at two depths and two times during the day. With this approach we attempted to capture the transcriptional diversity associated with change in light and other depth-related environmental factors. Using this extensive data set we generated gene predictions and identified an extensive catalogue of potential Simple Sequence Repeats (SSR) markers. The data generated here will open new avenues for the analysis of population genetic features and functional variation in P. oceanica. In total, 79,235 contigs were obtained by the assembly of 70,453,120 paired end reads. 43,711 contigs were successfully annotated. A total of 17,436 SSR were identified within 13,912 contigs.

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Section: Background and Summarymentioning

confidence: 99%

Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

et al. 2016

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“…2009; Illumina RNA‐sequencing BioProject ID: PRJNA315106 at DDBJ/ENA/GenBank, under the accession GEMD01000000.) were scored to select suitable targets belonging to the topics: “Photosynthesis,” “Photoprotection,” “Carbon metabolism,” “Photoreceptors,” and “Photoperiod.” Among the selected genes (Table S1), fourteen ones were already investigated in other studies (i.e., Dattolo et al., 2014; Lauritano et al., 2015), while the other sixteen were tested for the first time in this work.…”

Section: Methodsmentioning

confidence: 99%

“…Indeed, a consistent level of genetic differentiation between depths has been recorded in various populations (D'Esposito, Dattolo, Badalamenti, Orsini, & Procaccini, 2012; Migliaccio et al., 2005; Procaccini et al., 2001). Moreover, plants growing at different depths show differences in their response to temperature (Marín‐Guirao, Ruiz, Dattolo, Garcia‐munoz, & Procaccini, 2016) and in many phenotypic characters also affecting meadow structure (Zupo et al., 2006), such as: leaf morphology (Dalla Via et al., 1998), photo‐physiology (Dattolo et al., 2013, 2014; Mazzuca et al., 2013; Pirc, 1986), growth, and respiration (Olesen, Enriquez, Duarte, & Sand‐Jensen, 2002). …”

Section: Introductionmentioning

confidence: 99%

Long‐term acclimation to reciprocal light conditions suggests depth‐related selection in the marine foundation speciesPosidonia oceanica

Dattolo

Marín-Guirao

Ruiz

et al. 2017

Ecology and Evolution

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Phenotypic differences among populations of the same species reflect selective responses to ecological gradients produced by variations in abiotic and biotic factors. Moreover, they can also originate from genetic differences among populations, due to a reduced gene flow. In this study, we examined the extent of differences in photo‐acclimative traits of Posidonia oceanica (L.) Delile clones collected above and below the summer thermocline (i.e., −5 and −25 m) in a continuous population extending along the water depth gradient. During a reciprocal light exposure and subsequent recovery in mesocosms, we assessed degree of phenotypic plasticity and local adaptation of plants collected at different depths, by measuring changes in several traits, such as gene expression of target genes, photo‐physiological features, and other fitness‐related traits (i.e., plant morphology, growth, and mortality rates). Samples were also genotyped, using microsatellite markers, in order to evaluate the genetic divergence among plants of the two depths. Measures collected during the study have shown a various degree of phenotypic changes among traits and experimental groups, the amount of phenotypic changes observed was also dependent on the type of light environments considered. Overall plants collected at different depths seem to be able to acclimate to reciprocal light conditions in the experimental time frame, through morphological changes and phenotypic buffering, supported by the plastic regulation of a reduced number of genes. Multivariate analyses indicated that plants cluster better on the base of their depth origin rather than the experimental light conditions applied. The two groups were genetically distinct, but the patterns of phenotypic divergence observed during the experiment support the hypothesis that ecological selection can play a role in the adaptive divergence of P. oceanica clones along the depth gradient.

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“…Primer efficiencies were calculated for each oligo pair generating standard curves with five dilution points by using the cycle threshold (Ct) value versus the logarithm of each dilution factor and using the equation E = 10 −1/slope . RT-qPCR was performed as in Dattolo et al (2014). Control sites (S1 pH 8.14 and 8.17, for Ischia and Panarea, respectively) were used as reference conditions.…”

Section: Reverse-transcription Quantitative Polymerase Chain Reactionmentioning

confidence: 99%

Response of key stress-related genes of the seagrass <i>Posidonia oceanica</i> in the vicinity of submarine volcanic vents

et al. 2015

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Abstract. Submarine volcanic vents are being used as natural laboratories to assess the effects of increased ocean acidity and carbon dioxide (CO 2 ) concentration on marine organisms and communities. However, in the vicinity of volcanic vents other factors in addition to CO 2 , which is the main gaseous component of the emissions, may directly or indirectly confound the biota responses to high CO 2 . Here we used for the first time the expression of antioxidant and stress-related genes of the seagrass Posidonia oceanica to assess the stress levels of the species. Our hypothesis is that unknown factors are causing metabolic stress that may confound the putative effects attributed to CO 2 enrichment only. We analyzed the expression of 35 antioxidant and stressrelated genes of P. oceanica in the vicinity of submerged volcanic vents located in the islands of Ischia and Panarea, Italy, and compared them with those from control sites away from the influence of vents. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) was used to characterize gene expression patterns.Fifty-one percent of genes analyzed showed significant expression changes. Metal detoxification genes were mostly down-regulated in relation to controls at both Ischia and Panarea, indicating that P. oceanica does not increase the synthesis of heavy metal detoxification proteins in response to the environmental conditions present at the two vents. The up-regulation of genes involved in the free radical detoxification response (e.g., CAPX, SODCP and GR) indicates that, in contrast with Ischia, P. oceanica at the Panarea site faces stressors that result in the production of reactive oxygen species, triggering antioxidant responses. In addition, heat shock proteins were also activated at Panarea and not at Ischia. These proteins are activated to adjust stressaccumulated misfolded proteins and prevent their aggregation as a response to some stressors, not necessarily high temperature. This is the first study analyzing the expression of target genes in marine plants living near natural CO 2 vents. Our results call for contention to the general claim of seagrasses as "winners" in a high-CO 2 world, based on observations near volcanic vents. Careful consideration of factors that are at play in natural vents sites other than CO 2 and acidification is required. This study also constitutes a first step for using stress-related genes as indicators of environmental pressures in a changing ocean.

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Response of the seagrass Posidonia oceanica to different light environments: Insights from a combined molecular and photo-physiological study

Cited by 85 publications

References 85 publications

Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

Long‐term acclimation to reciprocal light conditions suggests depth‐related selection in the marine foundation speciesPosidonia oceanica

Response of key stress-related genes of the seagrass <i>Posidonia oceanica</i> in the vicinity of submarine volcanic vents

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Response of the seagrass Posidonia oceanica to different light environments: Insights from a combined molecular and photo-physiological study

Cited by 85 publications

References 85 publications

Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

Transcriptome characterisation and simple sequence repeat marker discovery in the seagrass Posidonia oceanica

Long‐term acclimation to reciprocal light conditions suggests depth‐related selection in the marine foundation speciesPosidonia oceanica

Response of key stress-related genes of the seagrass &lt;i&gt;Posidonia oceanica&lt;/i&gt; in the vicinity of submarine volcanic vents

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Response of key stress-related genes of the seagrass <i>Posidonia oceanica</i> in the vicinity of submarine volcanic vents