Response of soil respiration to short-term changes in precipitation and nitrogen addition in a desert steppe

Abstract

Changes in precipitation and nitrogen (N) addition may significantly affect the processes of soil carbon (C) cycle in terrestrial ecosystems, such as soil respiration. However, relatively few studies have investigated the effects of changes in precipitation and N addition on soil respiration in the upper soil layer in desert steppes. In this study, we conducted a control experiment that involved a field simulation from July 2020 to December 2021 in a desert steppe in Yanchi County, China. Specifically, we measured soil parameters including soil temperature, soil moisture, total nitrogen (TN), soil organic carbon (SOC), soil microbial biomass carbon (SMBC), soil microbial biomass nitrogen (SMBN), and contents of soil microorganisms including bacteria, fungi, actinomyces, and protozoa, and determined the components of soil respiration including soil respiration with litter (RS+L), soil respiration without litter (RS), and litter respiration (RL) under short-term changes in precipitation (control, increased precipitation by 30%, and decreased precipitation by 30%) and N addition (0.0 and 10.0 g/(m 2 a)) treatments. Our results indicated that short-term changes in precipitation and N addition had substantial positive effects on the contents of TN, SOC, and SMBC, as well as the contents of soil actinomyces and protozoa. In addition, N addition significantly enhanced the rates of RS+L and RS by 4.8% and 8.0% (P<0.05), respectively. The increase in precipitation markedly increased the rates of RS+L and RS by 2.3% (P<0.05) and 5.7% (P<0.001), respectively. The decrease in precipitation significantly increased the rates of RS+L and RS by 12.9% (P<0.05) and 23.4% (P<0.001), respectively. In contrast, short-term changes in precipitation and N addition had no significant effects on RL rate (P>0.05). The mean RL/RS+L value observed under all treatments was 27.63%, which suggested that RL is an important component of soil respiration in the desert steppe ecosystems. The results also showed that short-term changes in precipitation and N addition had significant interactive effects on the rates of RS+L, RS, and RL (P<0.001). In addition, soil temperature was the most important abiotic factor that affected the rates of RS+L, RS, and RL. Results of the correlation analysis demonstrated that the rates of RS+L, RS, and RL were closely related to soil temperature, soil moisture, TN, SOC, and the contents of soil microorganisms, and the structural equation model revealed that SOC and SMBC are the key factors influencing the rates of RS+L, RS, and RL. This study provides further insights into the characteristics of soil C emissions in desert steppe ecosystems in the context of climate change, which can be used as a reference for future related studies.