Response of Soil Nitrogen Components and nirK- and nirS-Type Denitrifying Bacterial Community Structures to Drip Irrigation Systems in the Semi-Arid Area of Northeast China

Abstract

Denitrification is a key process in soil available nitrogen (N) loss. However, the effects of different water-saving irrigation systems on soil N components and denitrifying bacterial communities are still unclear. In this study, quantitative fluorescence PCR and Illumina MiSeq sequencing were used to investigate the effects of three main irrigation systems, conventional flooding irrigation (FP), shallow buried drip irrigation (DI), and mulched drip irrigation (MF), on the abundance, community composition, and diversity of soil nirK- and nirS-type denitrifying bacteria in the semi-arid area of Northeast China, and to clarify the driving factors of nirK- and nirS-type denitrifying bacterial community variations. The results showed that, compared with FP, MF significantly increased soil moisture, alkaline hydrolyzed nitrogen (AHN), nitrate nitrogen (NO3−-N), non-acid hydrolyzed nitrogen (AIN), and amino sugar nitrogen (ASN), but significantly decreased the contents of ammonium nitrogen (NH4+-N) and acid hydrolyzed ammonium nitrogen (AN). The irrigation system changed the relative abundance of the dominant genera of denitrifying bacteria, DI and MF significantly increased nitrate reductase (NR) and nitrite reductase (NiR) activities, and MF significantly increased the diversity of nirK- and nirS-type denitrifying bacteria but significantly decreased the richness. The community structure of nirK- and nirS-type denitrifying bacteria was significantly different among the three irrigation systems. NO3−-N was the main driving factor affecting the community structure of nirS-type denitrifying bacteria, and moisture significantly affected the community structure of nirK-type denitrifying bacteria. DI and MF significantly increased the abundance of nirK- and nirS-type denitrifying bacteria and also increased the abundance ratio of nirS/nirK genes. Therefore, although DI and MF significantly increased the abundance of denitrifying microorganisms, they did not lead to an increase in the N2O emission potential.