Response of Sheep Chondrocytes to Changes in Substrate Stiffness from 2 to 20 Pa: Effect of Cell Passaging

Sanz-Ramos, Patricia; Mora, Guillermo; Vicente-Pascual, Mikel; Ochoa, Ignacio; Alcaine, Clara; Moreno, Renata; Doblaré, M.; Izal-Azcárate, Iñigo

doi:10.3109/03008207.2012.762360

Cited by 33 publications

(23 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They noted that neurogenic differentiation was optimal at a stiffness of 0.1-1 kPa, myogenic differentiation at 8-17 kPa and osteogenic differentiation at 25-40 kPa. Several subsequent studies have shown how substrate stiffness affects several different cell types, including neuronal cells [134,135], chondrocytes [136], cardiomyocytes [137,138], dermal fibroblasts [139] and limbal stem cells [140]. Recently, Trappmann et al [141] suggested that substrates of different stiffness have differing protein anchorage densities and configurations that regulate stem cell fate through the mechanical feedback of cells attached to anchored proteins [141].…”

Section: Influence Of the Hydrogel On Cell Behaviourmentioning

confidence: 99%

Introduction to cell–hydrogel mechanosensing

2014

View full text Add to dashboard Cite

The development of hydrogel-based biomaterials represents a promising approach to generating new strategies for tissue engineering and regenerative medicine. In order to develop more sophisticated cell-seeded hydrogel constructs, it is important to understand how cells mechanically interact with hydrogels. In this paper, we review the mechanisms by which cells remodel hydrogels, the influence that the hydrogel mechanical and structural properties have on cell behaviour and the role of mechanical stimulation in cell-seeded hydrogels. Cell-mediated remodelling of hydrogels is directed by several cellular processes, including adhesion, migration, contraction, degradation and extracellular matrix deposition. Variations in hydrogel stiffness, density, composition, orientation and viscoelastic characteristics all affect cell activity and phenotype. The application of mechanical force on cells encapsulated in hydrogels can also instigate changes in cell behaviour. By improving our understanding of cell-material mechano-interactions in hydrogels, this should enable a new generation of regenerative medical therapies to be developed.

show abstract

Section: Influence Of the Hydrogel On Cell Behaviourmentioning

confidence: 99%

Introduction to cell–hydrogel mechanosensing

2014

View full text Add to dashboard Cite

show abstract

“…HIF-1␣ also acts as a central regulator of collagen production that allows chondrocytes to maintain their function as professional secretory cells in the hypoxic growth plate [27,28]. Hypoxia and HIF-1␣ not only induce the expression of SOX9, COL2A1, and aggrecan [29], but they simultaneously inhibit the expression of COL1A1, COL1A2, and COL3A1 [30]. HIF-1␣ is a survival factor for hypoxic chondrocytes [31], and it also negatively regulates cell proliferation [32].…”

Section: Hif-1␣ In Cartilage and Oamentioning

confidence: 99%

Role of HIF-1α and HIF-2α in osteoarthritis

2015

View full text Add to dashboard Cite

“…In the hypoxia growth plate, HIF-1α maintains chondrocytes function as professional secretory cells by regulating collagen production [5]. HIF-1α not only inhibits the expression of COL1A1, COL1A2 and COL3A1 [6] but also simultaneously induces the expression of SOX9, COL2A1 and aggrecan [7]. HIF-1α inhibits cell proliferation [8] and is a survival factor for hypoxic chondrocytes [9].…”

Section: Introductionmentioning

confidence: 99%

MicroRNA-146a Induced by Hypoxia Promotes Chondrocyte Autophagy through Bcl-2

Zhang¹,

Wang

Chu

et al. 2015

Cell Physiol Biochem

View full text Add to dashboard Cite

Background/Aims: There have been many studies on the etiology of osteoarthritis (OA) with regard to the function of inflammatory cytokines, the process of cartilage degradation, the function of miR-146a, hypoxia stimulation and autophagy in OA chondrocytes, but there have been no reports on the relationship between miR-146a and autophagy in cartilage, especially under hypoxia. This study aimed to confirm the relationship of miR-146a and autophagy in cartilage under hypoxia. Methods: Chondrocytes were treated by hypoxia gradients, and the main factors including HIF-1α, HIF-2α, miR-146a and Bcl-2 and autophagy markers ULK-1, ATG-5 were detected by quantitative PCR (Q-PCR) and western blotting. The autophagy marker LC-3 was detected by immunofluorescence. The reciprocal effects between miR-146a and Bcl-2 were confirmed by several combinations of shRNAs and adenovirus-gene systems followed by Q-PCR and western blot detection. Results: Hypoxia maintained the chondrocytes phenotype and promoted autophagy and miR-146a expression via HIF-1α, but not HIF-2α, while miR-146a did not reversely affect HIF-1α. The autophagy induced by hypoxia through HIF-1α, miR-146a and Bcl-2. Simply, hypoxia induced HIF-1α, and HIF-1α increased miR-146a, but miR-146a suppressed Bcl-2, an autophagy inhibitor. While Bcl-2 affected neither HIF-1α nor miR-146a. The absence of both HIF-1α and miR-146a or Bcl-2 over-expression inhibited hypoxia-induced autophagy. Conclusion: HIF-1α, miR-146a and Bcl-2 play crucial roles during hypoxia-induced autophagy, Hypoxia, HIF-1α and miR-146a promote chondrocytes autophagy via depressing Bcl-2. We conclude that miR-146a may serve as a novel therapeutic target for protecting cartilage from degeneration in OA.

show abstract

Response of Sheep Chondrocytes to Changes in Substrate Stiffness from 2 to 20 Pa: Effect of Cell Passaging

Abstract: Chondrocytes respond differently according to the stiffness of the substrate. This response depends greatly on the oxygen environment and on whether the chondrocyte is embedded or grown onto the hydrogel, since mechanosensing capacity was not lost with cell expansion.

Cited by 33 publications

References 62 publications

Introduction to cell–hydrogel mechanosensing

Introduction to cell–hydrogel mechanosensing

Role of HIF-1α and HIF-2α in osteoarthritis

MicroRNA-146a Induced by Hypoxia Promotes Chondrocyte Autophagy through Bcl-2

Contact Info

Product

Resources

About