Response of Saos-2 osteoblast-like cells to kilohertz-resonance excitation in porous metallic scaffolds

Resonance vibration interventions in the femur: Experimental-numerical modelling approaches

“…This ex-vivo study follows previous work which investigated mechanics of in-vitro vibration bioreactors 25,26 , and used those to apply vibration excitation to cell cultures 27 . Tissue scaffolds for the mechanical stimulation of cells were analysed for their mechanical behaviour before that [28][29][30] .…”

“…In this study, a resonance vibration model for the bovine femur is presented. It builds up on previous studies which worked on in-vitro vibration bioreactors, and cell experiments [25][26][27]30 .…”

“…With regard to possible clinical applications, the quantification of experimentally obtainable strain is of relevance 1,27,30 . Figure 9 showed that the strain magnitude of 0.001 which is known to be beneficial for bone growth stimulation appears to be achievable for human anatomy 1,6 .…”

Nano-CT scans in the optimisation of purposeful experimental procedures: A study on metallic fibre networks

“…And of course, in-vitro studies which include cell cultures on 3d scaffolds are of interest. Greater controllability of geometry and deformation patterns will be achievable by the use of 3D printed scaffolds [67,68]. The integration of fibre network scaffolds into vibration bioreactors [69] offers manifold options for the controlled stimulation of tissue.…”

Implant resonance and the mechanostat theory: Applications of therapeutic ultrasound for porous metallic scaffolds

“…Specific to pseudarthrosis in bone, the use of low-intensity pulsed ultrasound (LIPUS) has been shown to possibly aid in bone regeneration and healing of non-unions 26,27 , where LIPUS treatment may be effective for de novo bone formation during fracture healing 28,29 and maturation of existing bone 30,31 within a defect site. A broad range of pulsed frequencies that spans the kilohertz 32 and megahertz [33][34][35] scales have been shown to induce favourable conditions for osteogenesis. In particular, LIPUS specific to bone applications has been shown to upregulate alkaline phosphatase 36,37 , osteocalcin [36][37][38] , and members of the transformation growth factor β superfamily 39,40 , among other growth factors.…”