Response of Root Growth and Development to Nitrogen and Potassium Deficiency as well as microRNA-Mediated Mechanism in Peanut (Arachis hypogaea L.)

Li, Lijie; Li, Qian; Davis, Kyle E.; Patterson, Caitlin; Oo, Sando; Liu, Wanying; Liu, Jia; Guo, Weihua; Fontana, Julia Elise; Thornburg, Thomas Elliott; Pratt, Isaac Seth; Li, Fēi; Zhang, Zhiyong; Zhou, Yanzhong; Pan, Xiaoping; Zhang, Baohong

doi:10.3389/fpls.2021.695234

Cited by 26 publications

(31 citation statements)

References 65 publications

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“…In this study, nitrogen additions improved chlorophyll content and Pn of moso bamboo seedling leaves, which led to a biomass increase for the whole plants ( Figure 1 ). Similar conclusions were reported in poplar, wheat ( Triticum aestivum ) ( Imran et al, 2019 ), and peanut ( Arachis hypogaea ) ( Li et al, 2021 ). Actually, in our study, the seedlings under N18 treatment had the most chlorophyll accumulation, while the seedlings under N12 had the highest Pn among the treatments ( Figure 1 ).…”

Section: Discussionsupporting

confidence: 79%

An Integrated Regulatory Network of mRNAs, microRNAs, and lncRNAs Involved in Nitrogen Metabolism of Moso Bamboo

Yuan

Zhu

et al. 2022

Front. Genet.

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Nitrogen is a key macronutrient essential for plant growth and development, and its availability has a strong influence on biological processes. Nitrogen fertilizer has been widely applied in bamboo forests in recent decades; however, the mechanism of nitrogen metabolism in bamboo is not fully elucidated. Here, we characterized the morphological, physiological, and transcriptome changes of moso bamboo in response to different schemes for nitrogen addition to illuminate the regulation mechanism of nitrogen metabolism. The appropriate addition of nitrogen improved the chlorophyll content and Pn (net photosynthetic rate) of leaves, the nitrogen and ammonium contents of the seedling roots, the biomass of the whole seedling, the number of lateral roots, and the activity of enzymes involved in nitrogen metabolism in the roots. Based on the whole transcriptome data of the roots, a total of 8,632 differentially expressed mRNAs (DEGs) were identified under different nitrogen additions, such as 52 nitrate transporter genes, 6 nitrate reductase genes, 2 nitrite reductase genes, 2 glutamine synthase genes, 2 glutamate synthase genes (GOGAT), 3 glutamate dehydrogenase genes, and 431 TFs belonging to 23 families. Meanwhile, 123 differentially expressed miRNAs (DEMs) and 396 differentially expressed lncRNAs (DELs) were characterized as nitrogen responsive, respectively. Furthermore, 94 DEM-DEG pairs and 23 DEL-DEG pairs involved in nitrogen metabolism were identified. Finally, a predicted regulatory network of nitrogen metabolism was initially constructed, which included 17 nitrogen metabolic pathway genes, 15 TFs, 4 miRNAs, and 10 lncRNAs by conjoint analysis of DEGs, DEMs, and DELs and their regulatory relationships, which was supported by RNA-seq data and qPCR results. The lncRNA-miRNA-mRNA network provides new insights into the regulation mechanism of nitrogen metabolism in bamboo, which facilitates further genetic improvement for bamboo to adapt to the fluctuating nitrogen environment.

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Section: Discussionsupporting

confidence: 79%

An Integrated Regulatory Network of mRNAs, microRNAs, and lncRNAs Involved in Nitrogen Metabolism of Moso Bamboo

Yuan

Zhu

et al. 2022

Front. Genet.

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“…N and K are the basic elements of crop growth and are essential for enhancing the quantity and quality of the harvested produce (Li et al, 2021b(Li et al, , 2021d. N affected crop growth externally by reducing leaf area and green leaf lifespan, and internally by inhibiting the synthesis of chlorophyll and protein to reduce photosynthetic products (Dos Santos et al, 2020;Mu and Chen, 2021).…”

Section: Experiments Designmentioning

confidence: 99%

Mechanical properties of stem and physiological–biochemical responses of cucumber under different N and K conditions

Liu

Wang

et al. 2022

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In order to investigate the effects of N and K on the mechanical properties of cucumber stems, the physiological and biochemical indexes under different N and K conditions were compared. The analysis showed that the pho-tosynthetic rate was affected by both stress and the self-senescence of cucumber plants, and that the crops under N and K stress were stimulated to activate a highly effective antioxidant system to cope with the damage. Sugar, as the basis for the synthesis and structure formation of the stems, was highly related to stem strength, and polyphenol oxidase in the study of stem strength was limited but indispensable. Compared with CK, the epidermal penetration strength under LN, LNLK, and LNHK treatments increased by at least 10%, while the strength under HN, LK, HNLK, and HNHK treatments decreased by at least 7%. To sum up, it was the photosynthesis rate, the sucrose transportation, as well as the futile cycling of K+ that were the keys of stem strength.

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“…Then, the extracted RNAs were stored at –80°C until further analysis. To reveal the miRNA-mediated mechanism under N deficiency, 20 miRNAs and their targets that associated with root development, nutrient stress in crops and reported in our previous studies were tested according to the method of previous reports ( Fontana et al, 2020 ; Thornburg et al, 2020 ; Li L. et al, 2021 ). Three biological replicates were run for each treatment.…”

Section: Methodsmentioning

confidence: 99%

Differential Responses of Wheat (Triticum aestivum L.) and Cotton (Gossypium hirsutum L.) to Nitrogen Deficiency in the Root Morpho-Physiological Characteristics and Potential MicroRNA-Mediated Mechanisms

Xue

Liu

et al. 2022

Front. Plant Sci.

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Understanding the mechanism of crop response to nitrogen (N) deficiency is very important for developing sustainable agriculture. In addition, it is unclear if the microRNA-mediated mechanism related to root growth complies with a common mechanism in monocots and dicots under N deficiency. Therefore, the root morpho-physiological characteristics and microRNA-mediated mechanisms were studied under N deficiency in wheat (Triticum aestivum L.) and cotton (Gossypium hirsutum L.). For both crops, shoot dry weight, plant dry weight and total leaf area as well as some physiological traits, i.e., the oxygen consuming rate in leaf and root, the performance index based on light energy absorption were significantly decreased after 8 days of N deficiency. Although N deficiency did not significantly impact the root biomass, an obvious change on the root morphological traits was observed in both wheat and cotton. After 8 days of treatment with N deficiency, the total root length, root surface area, root volume of both crops showed an opposite trend with significantly decreasing in wheat but significantly increasing in cotton, while the lateral root density was significantly increased in wheat but significantly decreased in cotton. At the same time, the seminal root length in wheat and the primary root length in cotton were increased after 8 days of N deficiency treatment. Additionally, the two crops had different root regulatory mechanisms of microRNAs (miRNAs) to N deficiency. In wheat, the expressions of miR167, miR319, miR390, miR827, miR847, and miR165/166 were induced by N treatment; these miRNAs inhibited the total root growth but promoted the seminal roots growth and lateral root formation to tolerate N deficiency. In cotton, the expressions of miR156, miR167, miR171, miR172, miR390, miR396 were induced and the expressions of miR162 and miR393 were inhibited; which contributed to increasing in the total root length and primary root growth and to decreasing in the lateral root formation to adapt the N deficiency. In conclusion, N deficiency significantly affected the morpho-physiological characteristics of roots that were regulated by miRNAs, but the miRNA-mediated mechanisms were different in wheat and cotton.

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Response of Root Growth and Development to Nitrogen and Potassium Deficiency as well as microRNA-Mediated Mechanism in Peanut (Arachis hypogaea L.)

Cited by 26 publications

References 65 publications

An Integrated Regulatory Network of mRNAs, microRNAs, and lncRNAs Involved in Nitrogen Metabolism of Moso Bamboo

An Integrated Regulatory Network of mRNAs, microRNAs, and lncRNAs Involved in Nitrogen Metabolism of Moso Bamboo

Mechanical properties of stem and physiological–biochemical responses of cucumber under different N and K conditions

Differential Responses of Wheat (Triticum aestivum L.) and Cotton (Gossypium hirsutum L.) to Nitrogen Deficiency in the Root Morpho-Physiological Characteristics and Potential MicroRNA-Mediated Mechanisms

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