Response of Rats with Gastric Fistulas to Injection of Gastrin.

Adashek, Kenneth; Grossman, Morton I.

doi:10.3181/00379727-112-28123

Cited by 40 publications

(9 citation statements)

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“…As we can see from table II, our results agree with those already published (1,4,11,(17)(18)(19)(20)(21). In fact, it has been shown that the doses which induce a maximal stimulation in rat vary considerably from author to author between 4 infusion of saline at the speed of 2.2 ml/h, doubling the dose of secretagogue each hour and sampling the gastric secretion every 30 min -a method which corre sponds to our step-dose technique.…”

Section: Discussionsupporting

confidence: 83%

Comparison of the Step-Dose and Single-Dose Acid Response to Histamine and Pentagastrin in Chronic Gastric Fistula Rats

Pascaud¹,

Roger²,

Genton³

1977

Digestion

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Time-response and dose-response curves for acid output to continuous infusion of histamine (HIST) and pentagastrin (PG) were determined by two techniques in conscious rats provided with chronic gastric fistula. With the step-dose technique the dose of the stimulant was increased each hour; with the single-dose technique only one dose of the stimulant was administered on each test day. The observed maximum acid output (OMAO) was obtained either by infusion of PG at a rate of 6 μg/kg/h using both techniques or HIST at a rate of 1 mg/kg/h with the single-dose and 2 mg/kg/h with the step-dose technique. These OMAO were in the order: single PG > single HIST > step HIST > step PG in ratios 100 – 99 – 83 – 82. The dose-response curves were also analyzed by application of the Michaelis-Menten equation. For each stimulant there was no difference between the calculated maximum acid output (CMAO) whatever the technique used, but PG gave lower CMAO than HIST (p < 0.05). For both stimulants the calculated doses for half the calculated maximal response (CD 50) are significantly higher with the step-dose than with the single-dose technique. These results are compared with those previously described in the literature for cats, dogs and man.

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Section: Discussionsupporting

confidence: 83%

Comparison of the Step-Dose and Single-Dose Acid Response to Histamine and Pentagastrin in Chronic Gastric Fistula Rats

Pascaud¹,

Roger²,

Genton³

1977

Digestion

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“…The same observation was made by Adashek & Grossman (1963) Schayer & Ivy (1957) concluded that histamine released from the rat mucosa is a blood-borne gastric secretory hormone. They injected fasted rats with 14C-histidine and with aminoguanidine to inhibit bistaminase.…”

Section: Discussionsupporting

confidence: 74%

Mobilization and formation of histamine in the gastric mucosa as related to acid secretion

Kahlson¹,

Rosengren²,

Svahn³

et al. 1964

The Journal of Physiology

279

107

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“…Similar differences have been encountered in the rat following stimulation by gastrin and histamine (Kahlson, Rosengren, Svahn, and Thunberg, 1964;Adashek and Grossman, 1963). If it becomes accepted that gastrin acts by releasing histamine, then, as Kahlson points out, the difference may represent an intracellular mobilization of histamine in proximity to the receptor sites, thus achieving a higher local concentration than is possible with histamine reaching the secretory cell from the outside.…”

Section: O~~~~~~~~~~~~~~~~o°r Elationship Of the Responses Of The Twomentioning

confidence: 75%

A comparative study of the effects of gastrin, histamine, histalog, and mechothane on the secretory capacity of the human stomach in two normal subjects over 20 months.

Makhlouf¹,

McManus²,

Card³

1965

Gut

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EDITORIAL SYNOPSIS This is an exhaustive study of the basal and stimulated secretion of two normal subjects over a period of 20 months. The calculated maximal responses have been experimentally obtained by slow intravenous administration of massive doses of gastrin It. These studies have enabled many characteristics of gastric activity to be defined.The gastric secretory acid responses to 'maximal' stimulation by histamine or gastrin II administered subcutaneously have previously been studied in normal individuals and ulcer patients (Makhlouf, McManus, and Card, 1964a). The dose-response of a normal subject to a series of continuous infusions or to graded single intravenous injections of gastrin II have also been reported (Makhlouf, McManus, and Card, 1964b).The present paper is an extension of these studies. The secretory responses of two normal male subjects, G.M. and J.M., have now been examined over a period of 20 months. The experiments were designed to determine the pattern of secretory response in time to various gastric stimulants, singly or in combination; and the relationship of the responses of the two subjects. In a study of this kind, two important parameters against which all data are to be interpreted must first be established with sufficient accuracy, namely, a base line respresenting the spontaneous secretion, and the maximal response of the stomach which, it will be shown subsequently, is defined by the value of the asymptote of the doseresponse curve following stimulation by intravenous gastrin. The first section will deal with the way in which these parameters are established. METHODSThe procedures have already been described in our previous communications. Three points, however, are worthy of re-emphasis.1 Salivary contamination was prevented by the insertion of dental cotton pledgets in the sulci of the cheeks.2 The juice was collected every five minutes and pooled into samples corresponding to 10-minute periods for purposes of titration, except in the case of single intravenous injections of gastrin, when five-minute samples were estimated directly.3 The 'pumping' effect of deep respiration is of major importance in the accurate collection of juice over short periods, and it was used throughout.In the early experiments, the spontaneous secretion was collected for one hour. As the subjects became conditioned to the procedure, it was found that a 20-minute collection, accurately recorded, provided a sufficient indication of the spontaneous secretion.All the tests, often repeated, were performed in a random manner over a period of 20 months. Fifty tests involving administration of gastrin IL by infusion or single intravenous injection were performed, and were equally distributed between the two subjects.The range of doses delivered by continuous infusion was 0-2 to 90 4g./hr. In the case of single intravenous injections of gastrin, the range was 0 5 to 100 /tg. total dose. In earlier experiments using this latter technique, insufficient attention had been paid to the standardization of the ti...

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Response of Rats with Gastric Fistulas to Injection of Gastrin.

Cited by 40 publications

References 0 publications

Comparison of the Step-Dose and Single-Dose Acid Response to Histamine and Pentagastrin in Chronic Gastric Fistula Rats

Comparison of the Step-Dose and Single-Dose Acid Response to Histamine and Pentagastrin in Chronic Gastric Fistula Rats

Mobilization and formation of histamine in the gastric mucosa as related to acid secretion

A comparative study of the effects of gastrin, histamine, histalog, and mechothane on the secretory capacity of the human stomach in two normal subjects over 20 months.

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