Response of Photosynthesis in Wheat (Triticum aestivum L.) Cultivars to Moderate Heat Stress at Meiosis and Anthesis Stages

Abstract

High temperature has seriously impacted the production of wheat in many countries. We examined four wheat cultivars (PBW343, Berkurt, Janz, and Attila) under heat stress (35/25 °C) and control treatments (23/15 °C) for 3 days at the meiosis and anthesis stages to evaluate the response and recovery of the four cultivars to heat stress and the relationship between photosynthetic parameters related to heat tolerance. Photosynthetic activity in all cultivars declined in plants that were treated at 35 °C, even for only 1 d compared with control plants. However, the differences among the four cultivars were obvious in net photosynthetic rate (Pn). At meiosis, the reduction of Pn in Berkut and PBW343 was lower and could nearly fully recover after 3 d of recovery and showed higher heat tolerance characteristics. The highest reduction in Pn occurred in Janz, which did not recover completely after 3 d of recovery. The same trend was observed at the anthesis stage, but Pn in all cultivars could not fully recover. Taking transpiration rate (Tr), stomatal conductance (gs), intercellular CO2 concentration (Ci), and limitation of stomatal conductance (Ls) into account, results suggested the decline in Pn under heat stress was mainly caused by non-stomatal restriction. In parallel with the decline in Pn, the maximum photochemical efficiency (Fv/Fm) decreased. In addition, both the maximum rate of net photosynthesis (Pmax) and the light saturation point declined after heat stress in all cultivars. However, the relevant photosynthetic parameters of PBW343 and Berkut recovered more quickly at both the meiotic and flowering stages. In summary, there were significant differences in the adaptability of different cultivars to high temperatures, with Berkut and PBW343 being more adaptable to heat stress than Janz and Attila. These may be used as valuable resources for further studies in breeding to understand the physiological mechanisms of heat sensitivity. This paper provides detailed information on the ecophysiological responses of wheat under heat stress.