Response of Peripheral Blood Th17 Cells to Inhaled Dermatophagoides pteronyssinus in Patients with Allergic Rhinitis and Asthma

Bajoriūnienė, Ieva; Malakauskas, Kęstutis; Lavinskienė, Simona; Jeroch, Jolanta; Gasiūnienė, Edita; Vitkauskienė, Astra; Sakalauskas, Raimundas

doi:10.1007/s00408-012-9411-y

Cited by 27 publications

(21 citation statements)

References 30 publications

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“…[4][5][6] In the present study, we observed an increased Th17 response in CD4 þ T cells from AR patients. These results have suggested that Th17 cells play a key role in the development of AR; the inhibition of Th17 cells is viewed as a promising approach for the treatment of AR.…”

Section: Discussionsupporting

confidence: 58%

“…4,16 We therefore further assessed the effect of ITE on Th17 cell differentiation and IL-17 production in CD4 þ…”

Section: Ite Inhibits Th17 Cell Differentiation and Il-17 Production mentioning

confidence: 99%

“…[4][5][6] Moreover, Ciprandi et al 5 revealed that serum IL-17 levels are also closely related to the clinical severity of AR. These results have indicated that Th17 may be involved in the immune response to casual allergens and could result in significant promotion of the severity of inflammatory disease.…”

mentioning

confidence: 99%

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An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

Wei

Kang

et al. 2014

Laboratory Investigation

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A predominant Th17 population is a marker of allergic rhinitis (AR). The aryl hydrocarbon receptor (AhR) exhibits strong immunomodulation potential via regulation of the differentiation of T lymphocytes and dendritic cells (DCs) after activation by its ligand, such as 2-(1 0 H-indole-3 0 -carbonyl)-thiazole-4-carboxylic acid methyl ester (ITE). The aim of this study was to analyze the effect of AhR on Th17 differentiation by investigating the action of ITE on DCs and CD4 þ T cells from patients with AR. In all, 26 AR patients and 12 healthy controls were included in this study. The expression of interleukin (IL)-1b, IL-6, IL-10, and IL-17 in the culture supernatant and the presence of Th17 cells in CD4þ T cells and DC-CD4 þ T-cell co-culture system were measured before and after treatment with ITE. We show that ITE significantly induced cell secretion of IL-10 and inhibited IL-1b and IL-6 production in DCs, and promoted IL-10 production and suppressed IL-17 expression in CD4 þ T cells in vitro. It also suppressed the expansion of Th17 cells in vitro. Our work demonstrates that ITE acts on DCs and CD4 þ T cells to inhibit the Th17 response that suppresses AR; the AhR-DC-Th17 axis may be an important pathway in the treatment of AR. ITE, a nontoxic AhR ligand, attenuated the Th17 response; thus, it appears to be a promising therapeutic candidate for suppressing the inflammatory responses associated with AR.

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Section: Discussionsupporting

confidence: 58%

“…4,16 We therefore further assessed the effect of ITE on Th17 cell differentiation and IL-17 production in CD4 þ…”

Section: Ite Inhibits Th17 Cell Differentiation and Il-17 Production mentioning

confidence: 99%

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An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

Wei

Kang

et al. 2014

Laboratory Investigation

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“…Similar increases in Th17 cells and IL-17 levels have been demonstrated with HDM extract stimulation [15,21]. In addition, levels of IL-17 mRNA in sputum from allergic asthmatics were demonstrated to correlate with levels of IL-5 mRNA [10].…”

Section: Discussionmentioning

confidence: 55%

“…In addition, Bajoriuniene et al [21] demonstrated a significant increase in circulating Th17 cells and serum IL-17 (but not its subtypes) in subjects with allergic asthma and allergic rhinitis compared to normal controls and a further increase after allergen inhalation challenge to HDM. Our study has identified that IL-17A, but not IL-17F, is increased in expression between asthmatics and normal controls and between pre- and postallergen challenge.…”

Section: Discussionmentioning

confidence: 99%

T Helper 17 Cells and Related Cytokines after Allergen Inhalation Challenge in Allergic Asthmatics

Naji

Smith

Gauvreau

et al. 2014

Int Arch Allergy Immunol

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Background: T helper (Th)17 cells may play a role in allergic asthma. This study assessed the effect of allergen inhalation challenge on circulating Th17 cells and related cytokines in allergic asthmatics. Methods: Peripheral blood mononuclear cells were collected from 16 atopic asthmatics before and 24 h after allergen challenge, as well as from 10 atopic nonasthmatics and 10 normal controls. Cells were stained for Th17 cytokines and their receptors (IL-17A, IL-17F, IL-21, IL-22, IL-17R, and IL-23R) using flow cytometry. Cytokine concentrations from cell culture supernatants were quantified using a multiplex assay for IL-17A, IL-17F, IL-21, IL-22, and IL-23. Results: At baseline, asthmatics had a higher percentage of circulating Th17 cells (1.2 ± 0.5%) compared to normal controls (0.9 ± 0.66%, p < 0.001) but not compared to atopic nonasthmatics (1.13 ± 0.5%). There was a significant increase in Th17 cells in asthmatics after allergen challenge to 1.55 ± 0.4% (p < 0.05) and a trend toward significance in IL-17R expression from 3.4 ± 4.3 to 6.86 ± 6.84% after allergen challenge (p = 0.06). There was also a significant reduction in IL-21-positive cells following allergen challenge from 3.46 ± 1.85 to 2.33 ± 1.37% (p < 0.001). There were no significant differences in IL-17F, IL-22 and IL-23R expression. The concentration of IL-17A in culture supernatant was significantly higher in asthmatics compared to normal controls and IL-17A significantly increased 24 h after allergen challenge. Conclusions: The increase of Th17 cells and IL-17A in atopic asthma after allergen inhalation challenge suggests a possible role for Th17 in allergen-induced airway responses.

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Semaphorin 3A inhibits allergic inflammation by regulating immune responses in a mouse model of allergic rhinitis

Xiang

Zhang

et al. 2018

Int Forum Allergy Rhinol

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Background: It has been reported that semaphorin 3A (sema3A) could improve allergic symptoms in allergic rhinitis (AR) mice. However, the immunomodulatory roles of sema3A in AR remain unclear. This study was performed to determine the immunoregulatory effects of sema3A on airway inflammation in an AR mice model. Methods:First, sema3A expression was measured in the serum of AR patients and also in a mice model. Then, nasal symptoms, ovalbumin (OVA)-specific immunoglobulin E (IgE) production, cytokine levels, and histologic structure were analyzed in OVA-sensitized mice, sema3A mice, mice given saline, and controls. The percentages of CD4 + IL-4 + IFN-γ -Th2 cells, CD4 + IFN-γ + IL-4 -Th1 cells, CD4 + IL-17 + Th17 cells, and CD4 + CD25 + Foxp3 + Treg cells in the spleen were also analyzed.Results: Serum sema3A levels in both AR patients and OVA-sensitized mice decreased significantly compared with controls. The intranasal administration of sema3A reduced allergic symptom scores, eosinophil infiltration, and OVA-specific IgE production in OVA-sensitized mice. In addition, levels of IL-4 and IL-17 as well as percentages of CD4 + IL-17 + Th17 cells were suppressed by sema3A administration. Levels of IFN-γ and IL-10 and ratios of CD4 + IFNγ + IL-4 -Th1/CD4 + IL-4 + IFN-γ -Th2 cells, as well as percentages of CD4 + CD25 + Foxp3 + Treg cells, were increased by administration of sema3A. Conclusion:Our results demonstrate that sema3A suppressed allergic inflammation in AR via inhibition of Th2/Th17 responses and enhancement of Th1/Treg responses. C 2018 ARS-AAOA, LLC. How to Cite thisArticle: Xiang R, Xu Y, Zhang W, et al. Semaphorin 3A inhibits allergic inflammation by regulating immune responses in a mouse model of allergic rhinitis. Int Forum Allergy Rhinol. 2019;9:528-537.

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Response of Peripheral Blood Th17 Cells to Inhaled Dermatophagoides pteronyssinus in Patients with Allergic Rhinitis and Asthma

Cited by 27 publications

References 30 publications

An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

An aryl hydrocarbon receptor ligand acts on dendritic cells and T cells to suppress the Th17 response in allergic rhinitis patients

T Helper 17 Cells and Related Cytokines after Allergen Inhalation Challenge in Allergic Asthmatics

Semaphorin 3A inhibits allergic inflammation by regulating immune responses in a mouse model of allergic rhinitis

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