Response of Pediculus humanus humanus (Pediculidae: Phthiraptera) to water or 70% ethanol immersion and determination of optimal times for measuring toxic effects

Cueto, G. Mougabure; Picollo, María Inés

doi:10.1007/s00436-010-1833-y

Cited by 13 publications

(3 citation statements)

References 14 publications

(20 reference statements)

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“…The dishes of treated groups were let for 10 min for air drying and then kept at temperature of 27°C and 75% relative humidity. All treated lice were examined for movement under stereo‐microscope after 2, 7, and 24 h according to (Mougabure and Picollo, 2010). The mortality of the treated lice was determined as lack of movement of the legs with no response if the lice were stroked by a needle and the mortality percentage was recorded (Khater et al, 2014).…”

Section: Methodsmentioning

confidence: 99%

D‐limonene nanoemulsion: lousicidal activity, stability, and effect on the cuticle of Columbicola columbae

Gadelhaq

Aboelhadid

Abdel‐Baki

et al. 2022

Medical Vet Entomology

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The current study was conducted to investigate the efficacy and stability of D-limonene (DL) and its nanoemulsion (DLN) against pigeon feather lice (Columbicola columbae) and their mode of action. DL pure form and DLN were prepared and characterized freshly and after storage for 50 days. In vitro bioassay on live lice was conducted with different concentrations of DL, DLN, and deltamethrin (DM). The results revealed significant mortality rates in the DL-, DLN-, DM-treated groups when compared with the control (p < 0.05). The scanning electron micrographs of lice treated with DL and DLN revealed collapsed bodies with destruction in the cuticle of the mouthparts and damaged antennae. The 50 days stored DLN showed stability in their effectiveness when compared with the freshly prepared formulation. DL and DLN caused significant inhibition (p ≤ 0.05) in acetylcholinesterase activity (AchE). Malondialdehyde level (MDA) was significantly increased while glutathione was significantly decreased in DL-and DLNtreated lice. In conclusion, DL and DLN have significant lousicidal activities. DLN showed better stability than DL after storage for 50 days. In addition, the mode of action of DL may associate with its effect on the cuticle of the lice body, inhibition of AchE, and increasing oxidative stress in the treated lice.

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Section: Methodsmentioning

confidence: 99%

D‐limonene nanoemulsion: lousicidal activity, stability, and effect on the cuticle of Columbicola columbae

Gadelhaq

Aboelhadid

Abdel‐Baki

et al. 2022

Medical Vet Entomology

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“…On one side, there is an increasing spread of resistance of head lice against long-used insecticides such as permethrin, allethrin, malathion, etc., while other compounds (e.g., lindane, malathion) became recently forbidden in several countries (Burgess 2004;Clark 2009;Downs et al 2002;Durand et al 2007;Falagas et al 2008;Gao et al 2006;Kristensen et al 2006;Lebwohl et al 2007;MougabureCueto et al 2008;Yoon et al 2004;Mehlhorn 2008Mehlhorn , 2011. Furthermore, the groups of kids in the institutions have been enlarged, so that there are closer and more often hairto-hair contacts among the individuals (Burkhart and Burkhart 2001;Toloza et al 2009;Mumcuoglu et al 2009;Mougabure-Cueto and Picollo 2010;Bauer et al 2009;Meinking et al 2002;Lebwohl et al 2007;Mehlhorn and Mehlhorn 2010). In addition, the high costs of anti-lice products (especially in the USA, where treatments cost US $150 upwards; Gur and Schneeweiss 2009) make people hesitate to buy and use effective products at an early stage of the louse infestation, so that infestations spread from these heads leading finally to repeated "ping-pong infestations" among group members.…”

Section: Introductionmentioning

confidence: 92%

Efficacy of a single treatment of head lice with a neem seed extract: an in vivo and in vitro study on nits and motile stages

et al. 2011

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An anti-louse shampoo (Licener®) based on a neem seed extract was tested in vivo and in vitro on its efficacy to eliminate head louse infestation by a single treatment. The hair of 12 children being selected from a larger group due to their intense infestation with head lice were incubated for 10 min with the neem seed extract-containing shampoo. It was found that after this short exposition period, none of the lice had survived, when being observed for 22 h. In all cases, more than 50-70 dead lice had been combed down from each head after the shampoo had been washed out with normal tap water. A second group of eight children had been treated for 20 min with identical results. Intense combing of the volunteers 7 days after the treatment did not result in the finding of any motile louse neither in the 10-min treated group nor in the group the hair of which had been treated for 20 min. Other living head lice were in vitro incubated within the undiluted product (being placed inside little baskets the floor of which consisted of a fine net of gauze). It was seen that a total submersion for only 3 min prior to washing 3× for 2 min with tap water was sufficient to kill all motile stages (larvae and adults). The incubation of nits at 30°C into the undiluted product for 3, 10, and 20 min did not show differences. In all cases, there was no eyespot development or hatching larvae within 7-10 days of observation. This and the fact that the hair of treated children (even in the short-time treated group of only 10 min) did not reveal freshly hatched larval stages of lice indicate that there is an ovicidal activity of the product, too.

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“…Combs are used not only for diagnosis but also as part of an integrated control strategy. This is especially relevant after the detection of high levels of insecticide resistance in head louse populations from several countries (Burgess 2004;Mougabure Cueto and Picollo 2010;Gallardo et al 2009). Previous studies have reported no significant differences in the effectiveness of two metal combs (Kurt et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Comparative efficacy of commercial combs in removing head lice (Pediculus humanus capitis) (Phthiraptera: Pediculidae)

et al. 2012

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The use of a fine comb for removing lice from the head of the human host is a relevant tool both in the diagnosis of infestations and as part of an integrated control strategy of head lice. The effectiveness of a fine comb depends, in part, on the design and material they are built. The aim of this study was to compare in vivo the efficacy of metal and plastic combs that are currently used in the removal of head lice and eggs worldwide. The space between comb teeth and the length was 0.23 and 13 mm in KSL® plastic, 0.3 and 10.7 mm in NOPUCID® plastic, 0.15 and 31 mm in KSL® metal and 0.09 and 37 mm in ASSY® metal. The assays were performed comparing the combs in pairs: (a) KSL® vs. NOPUCID® plastic combs, (b) KSL® vs. ASSY® metal combs and (c) KSL® plastic comb vs. ASSY® metal comb. The most effective plastic comb was KSL®, removing a higher number of individuals of all stages. The most effective metal comb was ASSY®, removing more insects of all stages (except adults). The comparative test between KSL® plastic and ASSY® metal showed that ASSY® was the most effective in removing head lice and their eggs.

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Response of Pediculus humanus humanus (Pediculidae: Phthiraptera) to water or 70% ethanol immersion and determination of optimal times for measuring toxic effects

Cited by 13 publications

References 14 publications

D‐limonene nanoemulsion: lousicidal activity, stability, and effect on the cuticle of Columbicola columbae

D‐limonene nanoemulsion: lousicidal activity, stability, and effect on the cuticle of Columbicola columbae

Efficacy of a single treatment of head lice with a neem seed extract: an in vivo and in vitro study on nits and motile stages

Comparative efficacy of commercial combs in removing head lice (Pediculus humanus capitis) (Phthiraptera: Pediculidae)

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