Response of peanut Arachis hypogaea roots to the presence of beneficial and pathogenic fungi by transcriptome analysis

Abstract

Entomopathogenic fungus Metarhizium anisopliae obtain survival benefit meanwhile promote the nutrient absorption of root as an endophyte. However, little is known concerning molecular mechanisms in the process. We performed the transcriptome sequencing of A. hypogaea roots inoculated M. anisopliae and pathogenic Fusarium axysporum, respectively. There were 81323 unigenes from 132023 transcripts. Total 203 differentially expressed genes (DEGs) respond to the two fungi, including specific 76 and 34 DEGs distributed respectively in M. anisopliae and F. axysporum treatment. KEGG pathway enrichment for DEGs showed the two top2 were signal transductions of plant-pathogen interaction and plant hormone. By qRT-PCR, the mRNA level of 26 genes involved in plant-fungus interaction confirmed the reliability of the RNA-Seq data. The expression pattern of the key DEGs on jasmonic acid (JA) or salicylic acid (SA) signaling pathway presented regulating consistency with JA or SA concentration detected by HPLC-MS. Those significantly stronger down-regulated DEGs by M. anisopliae thanby F. axysporum linking to hypersensitive response and negative regulation of defense, and those specific up-regulated genes in M. anisopliae treatment may predict that the less immunity is conducive to symbiosis F. axysporum may trigger JA-mediated defense regulated by ERF branch of JA signaling pathway, whereas M. anisopliae does not.

Comparative transcriptomic analysis of resistant and susceptible alfalfa cultivars (Medicago sativa L.) after thrips infestation

“…Alfalfa cultivars were used from each of the four treatments, RNA extraction, library construction and sequencing were carried out as described previously [ 27 ].…”

“…All the downstream analyses were based on clean data with high quality, and the methods for sequence reads and mapping could be referenced as Hao et al [ 27 ]. The left.fq and right.fq were used for the transcriptome assembly [ 27 ], and seven databases including Nt (NCBI non-redundant nucleotide sequences), Nr (NCBI non-redundant Protein sequences), Pfam (Protein family), KO (KEGG Ortholog database) and GO (Gene Ontology), KOG/COG (Clusters of Orthologous Groups of proteins), Swiss-Prot (a manually annotated and reviewed protein sequence database) were used for gene function annotation [ 27 ]. Data for each sequenced library was analyzed using BLAST with a cutoff E-value of 10 − 5 .…”

Association mapping of several nut characters in walnut (Juglansregia L.)

“…There were also several candidate genes identified to be associated with only one trait in the present study. Among these, Feruloyl CoA ortho-hydroxylase 1-like that play a role in oxidation-reduction process (Hao et al, 2017) were related to M7405 (Table 4). Protein PHR1-LIKE 2-like, which is responsible for stresses response (Li et al, 2016), was associated with M6734 (Table 9).…”

Unraveling the mechanisms of resistance to Sclerotium rolfsii in peanut (Arachis hypogaea L.) using comparative RNA-Seq analysis of resistant and susceptible genotypes

“…This technique is widely used for understanding host-pathogen interaction as well as elucidating plant defense pathways [13,14]. Moreover, it has been successfully used to unfold various developmental pathways [15], oil accumulation pathways [16], and in response to biotic [17] and abiotic stimuli in peanut [18,19]. An RNA-Seq transcriptome analysis of Brassica infected with Sclerotinia sclerotiorum revealed that the genes involved in pathogen recognition, MAPK signaling cascade, regulation of WRKY transcription factors, and jasmonic acid and ethylene signaling pathways, impart resistance [20,21].…”