1965
DOI: 10.1038/206319b0
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Response of Oxidation and Phosphorylation in Citrus Mitochondria to Arsenate

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Cited by 19 publications
(14 citation statements)
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“…A mitochondrial fraction was prepared by a modification of the standard method of Vines and Oberbacher (1965). Peeled oranges (endocarp) were homogenized in a household food blendor at high speed for 30 sec at 4OC with equal weight of neutralizing medium.…”
Section: Enzyme Extraction and Assaymentioning
confidence: 99%
“…A mitochondrial fraction was prepared by a modification of the standard method of Vines and Oberbacher (1965). Peeled oranges (endocarp) were homogenized in a household food blendor at high speed for 30 sec at 4OC with equal weight of neutralizing medium.…”
Section: Enzyme Extraction and Assaymentioning
confidence: 99%
“…Assay of ascorbate oxidase activity was carried out essentially as described by Vines and Oberbacher (18). The sample was homogenized (1: 5 (w/v)) with phosphate buffer (0.1 M/ pH 6.5) and centrifuged at 3000 g for 15 min at 50 o C. The supernatant obtained was used.…”
Section: Assay Of Ascorbate Oxidasementioning
confidence: 99%
“…Assay of Ascorbate oxidase activity was carried out according to the procedure of Vines et al, (1965).To 3.0 ml of ascorbate solution (0.003 %), 0.1 ml of extract was added and change in absorbance at 265 nm was measured at an interval of 30 sec for a period of 5 min. One unit of enzyme activity was expressed as 0.01 OD change per mg protein.…”
Section: Assay Of Ascorbate Oxidase:-mentioning
confidence: 99%