“…A mitochondrial fraction was prepared by a modification of the standard method of Vines and Oberbacher (1965). Peeled oranges (endocarp) were homogenized in a household food blendor at high speed for 30 sec at 4OC with equal weight of neutralizing medium.…”
Mature oranges were analyzed for citrate synthase, acyl coenzyme A reductase, acyl coenzyme A and palmitaldehyde. Peeled oranges contained 9640 units citrate synthase and 150 units of acyl coenzyme A reductase per kg. The level of fatty acyl coenzyme A (3 pM) was suggested as a potential effector of citrate synthase. Palmitaldehyde(0.04 pM) was suggested as a product of the acyl coenzyme A reductase reaction with palmityl coenzyme A.
“…A mitochondrial fraction was prepared by a modification of the standard method of Vines and Oberbacher (1965). Peeled oranges (endocarp) were homogenized in a household food blendor at high speed for 30 sec at 4OC with equal weight of neutralizing medium.…”
Mature oranges were analyzed for citrate synthase, acyl coenzyme A reductase, acyl coenzyme A and palmitaldehyde. Peeled oranges contained 9640 units citrate synthase and 150 units of acyl coenzyme A reductase per kg. The level of fatty acyl coenzyme A (3 pM) was suggested as a potential effector of citrate synthase. Palmitaldehyde(0.04 pM) was suggested as a product of the acyl coenzyme A reductase reaction with palmityl coenzyme A.
“…Assay of ascorbate oxidase activity was carried out essentially as described by Vines and Oberbacher (18). The sample was homogenized (1: 5 (w/v)) with phosphate buffer (0.1 M/ pH 6.5) and centrifuged at 3000 g for 15 min at 50 o C. The supernatant obtained was used.…”
“…Assay of Ascorbate oxidase activity was carried out according to the procedure of Vines et al, (1965).To 3.0 ml of ascorbate solution (0.003 %), 0.1 ml of extract was added and change in absorbance at 265 nm was measured at an interval of 30 sec for a period of 5 min. One unit of enzyme activity was expressed as 0.01 OD change per mg protein.…”
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