Response of osteoblasts and osteoclasts in regenerating scales to gravity loading

Suzuki, Nobuo; Kitamura, Kei-ichiro; Omori, Keisuke; Nemoto, Tetsu; Satoh, Yusuke; Tabata, M.; Ikegame, Mika; Yamamoto, Tetsuro; Ijiri, Kenichi; Furusawa, Yukihiro; Kondo, Takashi; Takasaki, Ichiro; Tabuchi, Yoshiaki; Wada, Shigehito; Shimizu, Nobuaki; Sasayama, Yuichi; Endo, Masato; Takeuchi, Toshio; Nara, Masayuki; Somei, Masanori; Maruyama, Yusuke; Hayakawa, Kazuichi; Shimazu, Toru; Shigeto, Yuko; Yano, Sachiko; Hattori, Atsuhiko

doi:10.2187/bss.23.211

Cited by 30 publications

(34 citation statements)

References 31 publications

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“…We believe that these bone-formative and antiresorptive effects of vibration on mRNA levels are directly linked to changes in protein levels, similar to those observed in our previous study of vibration-induced changes in bone-formative and antiresorptive enzymatic activities (Suzuki et al, 2009).…”

Section: Discussionsupporting

confidence: 86%

“…A cotton ball (diameter, 1 cm) was placed in each microtube above the scales to prevent induction of flow in the microtube during acceleration loading. The microtubes containing scales were loaded with vibrational 3G acceleration (consisting of alternate vertical 2G acceleration caused by vibration and 1G ground gravity) for 10 min at room temperature, using a vibration loading system, as reported in our previous study (Suzuki et al, 2009). After acceleration loading, scales from the 24 tubes were split into 4 groups, consisting of 6 tubes each, and the 4 groups were incubated at 15˚C for 3, 6, 12, and 24 h. After incubation, scales were frozen at −80˚C until analysis.…”

Section: Application Of Vibrationmentioning

confidence: 99%

“…In our previous study (Suzuki et al, 2009), we examined the effects of acceleration loading by vibration on osteoblastic and osteoclastic marker enzyme activities. We found that osteoblastic marker enzyme activity in the regenerating scales significantly increased under 3G acceleration, whereas osteoclastic marker enzyme activity in the loaded regenerating scales significantly decreased.…”

Section: Introductionmentioning

confidence: 99%

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Osteoblasts and Osteoclasts in Regenerating Goldfish Scales Respond to Mechanical Loading: Analysis of Osteoblastic and Osteoclastic Marker mRNA Expression

et al. 2012

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We aimed to investigate the effects of dynamic mechanical loading on the expression of osteoblast-and osteoclast-specific genes in regenerating goldfish scales. Fourteen days after the removal of scales from goldfish, the regenerating scales were found to contain calcified tissue, with osteoblasts, osteoclasts, and a newly synthesized calcified bone matrix plate. Recently, we found that goldfish s c a l e r e g e n e r a t i o n s h o w s s i m i l a r g e n e expression to intramembranous mammalian bone osteogenesis. Using such regenerating scales on day 14, we analyzed the effects of dynamic 3G acceleration, via application of a vibration stimulus, on the mRNA expression in osteoblasts and osteoclasts. In osteoblasts, vibration increased distal-less homeobox 5 (an osteoblastic transcriptional gene) mRNA expression at 3 h after stimulation and type I collagen (an osteoblastic functional gene) mRNA expression at 6 h and 12 h after stimulation. Moreover, vibration decreased the mRNA expression of the receptor activator of the NF-κB ligand, which binds to the osteoclast surface receptor and facilitates bone resorption, at 12 h and 24 h after stimulation in osteoblasts. Further, vibration decreased cathepsin K (an osteoclastic functional gene) mRNA expression at 12 h after stimulation. Thus, our results indicate that vibration drives osteoblastic and osteoclastic control of antiresorptive and boneformative effects through mRNA expression.

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Section: Discussionsupporting

confidence: 86%

Section: Application Of Vibrationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Osteoblasts and Osteoclasts in Regenerating Goldfish Scales Respond to Mechanical Loading: Analysis of Osteoblastic and Osteoclastic Marker mRNA Expression

et al. 2012

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“…Osteoblastic activity in the regenerating scale was considerably higher than that in normal scale (Yoshikubo et al, 2005;Suzuki et al, 2009). The response of osteoblasts to estrogen was higher in regenerating scales than in normal scales (Yoshikubo et al, 2005).…”

mentioning

confidence: 75%

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish,Carassius auratus

Suzuki¹,

Sato²,

Nassar³

et al. 2016

Zoological Science

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We have developed an original in vitro bioassay using teleost scale that has osteoclasts, osteoblasts, and bone matrix as each marker: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Using this scale in vitro bioassay, we examined the effects of seawater polluted with highly concentrated polycyclic aromatic hydrocarbons (PAHs) and nitro-polycyclic aromatic hydrocarbons (NPAHs) on osteoblastic and osteoclastic activities in the present study. Polluted seawater was collected from 2 sites (the Alexandria site on the Mediterranean Sea and the Suez Canal site on the Red Sea). Total levels of PAHs in the seawater from the Alexandria and Suez Canal sites were 1364.59 and 992.56 ng/l, respectively. We can detect NPAHs in both seawater samples. Total levels of NPAHs were detected in the seawater of the Alexandria site (12.749 ng/l) and the Suez Canal site (3.914 ng/l). Each sample of polluted seawater was added into culture medium at dilution rates of 50, 100, and 500 and incubated with the goldfish scales for 6 hrs. Thereafter, ALP and TRAP activities were measured. As a result, ALP activity was significantly suppressed by both polluted seawater samples diluted at least 500 times, although TRAP activity did not change. In addition, mRNA expressions of osteoblastic markers (ALP, osteocalcin, and the receptor activator of the NF-B ligand) decreased significantly, as did the ALP enzyme activity. Actually, ALP activity decreased with selected PAHs and NPAHs treatments. We conclude that seawater polluted with highly concentrated PAHs and NPAHs influenced bone metabolism in teleosts.

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“…Afterward, TRAP and ALP activities were normalized to the surface area (mm 2 ) of each scale (Suzuki et al, 2009). …”

Section: Morphological Study Of Osteoclasts and Osteoblasts In The Scmentioning

confidence: 99%

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

et al. 2014

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To evaluate the effects of inorganic mercury (InHg) and methylmercury (MeHg) on bone metabolism in a marine teleost, the activity of tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) as indicators of such activity in osteoclasts and osteoblasts, respectively, were examined in scales of nibbler fish (Girella punctata). We found several lines of scales with nearly the same TRAP and ALP activity levels. Using these scales, we evaluated the influence of InHg and MeHg. TRAP activity in the scales treated with InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) during 6 hrs of incubation decreased significantly. In contrast, ALP activity decreased after exposure to InHg (10 -5 and 10 -4 M) and MeHg (10 -6 to 10 -4 M) for 18 and 36 hrs, although its activity did not change after 6 hrs of incubation. As in enzyme activity 6 hrs after incubation, mRNA expression of TRAP (osteoclastic marker) decreased significantly with InHg and MeHg treatment, while that of collagen (osteoblastic marker) did not change significantly. At 6 hrs after incubation, the mRNA expression of metallothionein, which is a metal-binding protein in osteoblasts, was significantly increased following treatment with InHg or MeHg, suggesting that it may be involved in the protection of osteoblasts against mercury exposure up to 6 hrs after incubation. To our knowledge, this is the first report of the effects of mercury on osteoclasts and osteoblasts using marine teleost scale as a model system of bone.

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Response of osteoblasts and osteoclasts in regenerating scales to gravity loading

Cited by 30 publications

References 31 publications

Osteoblasts and Osteoclasts in Regenerating Goldfish Scales Respond to Mechanical Loading: Analysis of Osteoblastic and Osteoclastic Marker mRNA Expression

Osteoblasts and Osteoclasts in Regenerating Goldfish Scales Respond to Mechanical Loading: Analysis of Osteoblastic and Osteoclastic Marker mRNA Expression

Seawater Polluted with Highly Concentrated Polycyclic Aromatic Hydrocarbons Suppresses Osteoblastic Activity in the Scales of Goldfish,Carassius auratus

Effects of Inorganic Mercury and Methylmercury on Osteoclasts and Osteoblasts in the Scales of the Marine Teleost as a Model System of Bone

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