Response of Odorant Receptors with Phenylacetaldehyde and the Effects on the Behavior of the Rice Water Weevil (<i>Lissorhoptrus oryzophilus</i>)

Zhang, Xinxin; Wang, Xiao; Zhao, Shuaiguo; Fang, Kui; Wang, Zhun; Liu, Jianan; Xi, Jinghui; Wang, Shang; Zhang, Juhong

doi:10.1021/acs.jafc.2c07963

Cited by 9 publications

(6 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To verify the function of PstrOR17 in vivo, we employed RNAi-based gene silencing to silence PstrOR17 expression. Recently, RNAi has been successfully used in functional studies for downregulation of target genes for several insect species, particularly chemosensory genes [28,33,50]. Numerous studies reported that knockdown of ORs consistently produces attenuated behavioral responses.…”

Section: Discussionmentioning

confidence: 99%

“…The electroantennogram (EAG) procedure was followed as previously described with minor modifications [33]. Briefly, the head was excised and the proximal tip of the antenna was finely transected.…”

Section: Electroantennogrammentioning

confidence: 99%

“…Moreover, the olfactory mechanisms underlying sex pheromone communication have been elucidated in Campoletis chlorideae and Microplitis mediator [28][29][30]. However, besides 15 ORs characterized functionally across a few species, the functions of ORs in the largest insect order, Coleoptera, remain largely unexplored [22,23,[31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antenna-Biased Odorant Receptor PstrOR17 Mediates Attraction of Phyllotreta striolata to (S)-Cis-Verbenol and (−)-Verbenone

Xu,

Chen,

Yan

et al. 2024

IJMS

View full text Add to dashboard Cite

Phyllotreta striolata, the striped flea beetle, is one of the most destructive pests in Brassicaceae plants worldwide. Given the drawbacks associated with long-term use of chemical insecticides, green strategies based on chemical ecology are an effective alternative for beetle control. However, the lack of information on beetle ecology has hindered the development of effective biocontrol strategies. In this report, we identified two odorants, (S)-cis-verbenol and (−)-verbenone, which displayed significant attraction for P. striolata (p < 0.05), indicating their great potential for P. striolata management. Using the Drosophila “empty neuron” system, an antenna-biased odorant receptor, PstrOR17, was identified as responsible for the detection of (−)-verbenone and (S)-cis-verbenol. Furthermore, the interactions between PstrOR17 and (−)-verbenone or (S)-cis-verbenol were predicted via modeling and molecular docking. Finally, we used RNAi to confirm that PstrOR17 is essential for the detection of (−)-verbenone and (S)-cis-verbenol to elicit an attraction effect. Our results not only lay a foundation for the development of new and effective nonchemical insecticide strategies based on (S)-cis-verbenol and (−)-verbenone, but also provide new insight into the molecular basis of odorant recognition in P. striolata.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Electroantennogrammentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antenna-Biased Odorant Receptor PstrOR17 Mediates Attraction of Phyllotreta striolata to (S)-Cis-Verbenol and (−)-Verbenone

Xu,

Chen,

Yan

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…As an excellent indicator of flavor, PAA is a common compound emitted from numerous flowering plant species. Several behavioral studies have shown that PAA is extensively attractive to various agricultural insect pests. , Thus, PAA could be a potential trap component for controlling S. frugiperda infestation.…”

Section: Discussionmentioning

confidence: 99%

“…Phenylacetaldehyde (PAA) is a commonly emitted compound by numerous flowering plant species correlated with nectar sugar and pollen amounts. Additionally, PAA is also released by many important crops like maize, cotton, and tomato. − Several studies suggest that PAA plays an important role in chemosensory behavior in different groups of insects. A Y-tube behavioral assay indicates that PAA significantly attracts rice water weevil ( Lissorhoptrus oryzophilus ) adults .…”

Section: Introductionmentioning

confidence: 99%

Loss of Binding Capabilities in an Ecologically Important Odorant Receptor of the Fall Armyworm, Spodoptera frugiperda, by a Single Point Mutation

Liu,

Shi,

Khashaveh

et al. 2023

J. Agric. Food Chem.

View full text Add to dashboard Cite

Olfaction plays a crucial role in locating food sources, mates, and spawning sites in the fall armyworm (FAW), Spodoptera f rugiperda (Lepidoptera: Noctuidae). In the current study, SfruOR14, a highly conserved odorant receptor (OR) in lepidopteran species, was newly uncovered in S. f rugiperda. In two-electrode voltage clamp recordings, the SfruOR14/Orco complex was narrowly tuned to six volatile compounds including phenylacetaldehyde (PAA), benzaldehyde, heptaldehyde, (E)-2-hexen-1-al, cinnamaldehyde, and 2-phenylethanol, among which PAA showed the strongest binding affinity. Subsequent homology modeling and molecular docking revealed that Phe79, His83, Tyr149, Pro176, Gln177, Leu202, and Thr348 in SfruOR14 were the key binding residues against the six ligands. Finally, as a result of site-directed mutagenesis, the SfruOR14 His83Ala mutant completely lost its binding capabilities toward all ligands. Taken together, our findings provide valuable insights into understanding the interaction between SfruOR14 and the chemical ligands including PAA, which can help to design novel olfactory modulators for pest control.

show abstract

A conserved odorant receptor tuned to phenylacetaldehyde in Athetis lepigone

Fan,

Wang,

Liu

2024

New Plant Protection

View full text Add to dashboard Cite

Insects carry out various behaviors, including searching for food, mates, and oviposition sites using their sensitive olfactory systems. Odorant receptors (ORs) play critical roles during odor detection. In this study, we identified an OR (AlepOR14) in Athetis lepigone, which is clustered with a conserved HarmOR42‐lineage in Lepidoptera. We cloned this OR gene and investigated its expression levels using real‐time quantitative PCR. Our results indicated that the expression of AlepOR14 is biased toward antennae, with levels significantly higher in female antennae than in male antennae. Functional analysis using the Xenopus oocytes expression and voltage‐clamp recording system demonstrated that AlepOR14 robustly and sensitively responds to the critical floral scent volatile phenylacetaldehyde (PAA). In behavioral experiments, female adults are attracted by PAA. Our findings improve our general understanding of the relationship between moths and their host plants, and provide an idea for exploiting attractants of A. lepigone for biological control.

show abstract

Response of Odorant Receptors with Phenylacetaldehyde and the Effects on the Behavior of the Rice Water Weevil (Lissorhoptrus oryzophilus)

Cited by 9 publications

References 47 publications

Antenna-Biased Odorant Receptor PstrOR17 Mediates Attraction of Phyllotreta striolata to (S)-Cis-Verbenol and (−)-Verbenone

Antenna-Biased Odorant Receptor PstrOR17 Mediates Attraction of Phyllotreta striolata to (S)-Cis-Verbenol and (−)-Verbenone

Loss of Binding Capabilities in an Ecologically Important Odorant Receptor of the Fall Armyworm, Spodoptera frugiperda, by a Single Point Mutation

A conserved odorant receptor tuned to phenylacetaldehyde in Athetis lepigone

Contact Info

Product

Resources

About