Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition

Lincks, J.; Boyan, Boyanbuthscsa D.; Blanchard, Cheryl R.; Lohmann, Christoph H.; Liu, Y.; Cochran, David L.; Dean, David D.; Schwartz, Zvi

doi:10.1016/s0142-9612(98)00144-6

Cited by 639 publications

(322 citation statements)

References 45 publications

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“…These cells have been characterized and are used by our lab as a model for osteoblast response to titanium surfaces with varying topography [29, 30]. Surfaces were sterilized in UV for 20 minutes in a biosafety cabinet prior to cell culture.…”

Section: Methodsmentioning

confidence: 99%

Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner

et al. 2014

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Additive manufacturing by laser sintering is able to produce high resolution metal constructs for orthopaedic and dental implants. In this study, we used a human trabecular bone template to design and manufacture Ti-6Al-4V constructs with varying porosity via laser sintering. Characterization of constructs revealed interconnected porosities ranging from 15–70% with compressive moduli of 2063–2954 MPa. These constructs with macro porosity were further surface-treated to create a desirable multi-scale micro-/nano-roughness, which has been shown to enhance the osseointegration process. Osteoblasts (MG63 cells) exhibited high viability when grown on the constructs. Proliferation (DNA) and alkaline phosphatase specific activity (ALP), an early differentiation marker, decreased as porosity increased, while osteocalcin (OCN), a late differentiation marker, as well as osteoprotegerin (OPG), vascular endothelial growth factor (VEGF) and bone morphogenetic proteins 2 and 4 (BMP2, BMP4) increased with increasing porosity. 3D constructs with the highest porosity and surface modification supported the greatest osteoblast differentiation and local factor production. These results indicate that additively manufactured 3D porous constructs mimicking human trabecular bone and produced with additional surface treatment can be customized for increased osteoblast response. Increased factors for osteoblast maturation and differentiation on high porosity constructs suggest the enhanced performance of these surfaces for increasing osseointegration in vivo.

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Section: Methodsmentioning

confidence: 99%

Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner

et al. 2014

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“…The oxide layer is always present in oxidizing media as in human body fluid. 12,13 The TiO 2 layer formed by polishing is a thick oxide layer with a thickness of ϳ100 nm. A thicker titanium oxide film improves biocompatibility in implant materials.…”

Section: E80mentioning

confidence: 99%

Effect of Multi-nano-titania Film on Proliferation and Differentiation of Mouse Fibroblast Cell on Titanium

Lin

Chen

et al. 2008

J. Electrochem. Soc.

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This investigation studies how nano-͑␦-TiH, ␥-TiH 2 , and ␣-TiH 1.971 ͒ phases affect the formation of multi-nano-titania film by anodization and cathodic pretreatment. Nano-titanium hydrides and substoichiometric nano-titanium hydrides were formed during cathodization. A multi-nanoporous titania film was formed on the titanium during anodization. The nanohydrides are directly transformed to multi-nanoporous titania film by dissolution following anodization. Anodization with cathodic pretreatment not only yields a titanium surface with a multi-nanostructure, but also transforms the titanium surface into a nanostructured titania surface. Formation of nanohydrides by cathodization and oxidation by anodization are believed to promote biocompatibility and improve bone-to-interface contact, accelerating initial osseointegration and re-osseointegration.Commercially pure titanium ͑CP-Ti͒ and its alloy, Ti-6Al-4V, are widely applied in implant engineering, such as in artificial hips, dental implants, and orthopedic implants, owing to their excellent mechanical properties, biocompatibility, and neutral interference when modern imaging approaches are employed ͓computed tomography ͑CT͒, magnetic resonance imaging ͑MRI͔͒. 1-4 Titanium and its alloys are covered with a very stable surface oxide film when exposed to air or aqueous media, favoring the bone-binding capacity of titanium-based implants. 5 However, although commercially available titanium-based implants with stable surface oxide films with thicknesses of 1-5 nm have seen long-term clinical success, situations may still arise in which the oxide thickness of an implant must be increased to accelerate osseointegration. Therefore, various schemes have been presented to increase the surface oxide film of implants and promote bone-implant integration. 6-10 Additionally, implants are morphologically attached to the bone by bony ingrowth into the implant surface, which has a porous structure. 1,2 The synthesis of the extracellular matrix and subsequent mineralization in vitro are both significantly improved on porous-coated titanium. 11 Cells may be suitable for not only the surfaces of thick oxide film but also the surfaces with porous topographies. However, porous titania films have been prepared by immersing Ti in an alkaline solution for a long time. In this investigation, a procedure is adopted to form a nanoporous titania film on the surface of titanium bone plate. The properties of Ti with and without surface treatments were evaluated by electrochemical measurements and material analyses. Materials and MethodsPreparation of untreated and treated titanium bone plate.-The substrate was a Grade IV CP-Ti plate. The Ti was cut into plates with a thickness of 1 mm. The specimens were mechanically polished using 1500 grit paper and then using diamond abrasives to 1 m. The specimens were finished with colloidal silica abrasives to 0.04 m. All specimens were degreased by washing in acetone and prepickled in acid, being processed using 2% ammonium fluoride, a solution of 2% H...

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“…As one can see from Table 3, the MTT value on the Ti6Al4V alloy increased markedly from 0.070 to 0.081 when the surface roughness was increased from 0.21 µm in the untreated condition to 0.52 µm by mechanical roughening. In addition, θ was observed to decrease from 41.84 to 36.39 0 , which is perhaps not surprising as surface roughness is known to be a factor influential in determining θ, as demonstrated by Eqs (1) and (2). Significantly, Table 3 shows that the surface oxygen content and the γ sv [22] demonstrated that surface free energy was a more important surface characteristic than surface roughness for cellular adhesion strength and proliferation.…”

Section: Relationship Between Wettability Characteristics and Cell Rementioning

confidence: 73%

“…When titanium alloys are implanted into a complicated and aggressive physiological in vivo environment, the oxide stability might be affected, resulting in an increase metal ion release [2][3][4][5]. In order to improve of material surfaces can affect their wettability characteristics.…”

Section: Introductionmentioning

confidence: 99%

On the correlation between Nd:YAG laser-induced wettability characteristics modification and osteoblast cell bioactivity on a titanium alloy

Lawrence

Hao

Chew

2006

Surface and Coatings Technology

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The factors responsible for modifications to the wettability characteristics of a titanium (Ti6Al4V) alloy bio-metal following Nd:YAG laser treatment and the effects thereof on the response of osteoblast cells were considered in this work. It was found that interaction of the Nd:YAG laser beam with the Ti6Al4V alloy resulted in the wettability characteristics of the bio-metal improving. Such improvements in the wettability characteristics of the Ti6Al4V alloy were found to be due to: an increase in the surface roughness; and increase in the surface oxygen content and an increase in the polar component of the surface energy. From the cell response tests it was determined that the osteoblast cell adhesion and proliferation on the Nd:YAG laser treated Ti6Al4V alloy samples was considerably greater than on the untreated samples. By isolating the effects of surface roughness it was possible to confirm or refute the existence of a correlation between wettability characteristics and osteoblast cell bioactivity for the Nd:YAG laser treated Ti6Al4V alloy. The findings indicated that the aspects of wettability characteristics: surface oxygen content and polar component of the surface energy play an important role in promoting cell proliferation, particularly when surface roughness was simultaneously increased. Thus it was possible to conclude that the wettability characteristics of the Nd:YAG laser treated Ti6Al4V alloy were correlated to osteoblast cell bioactivity.

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Response of MG63 osteoblast-like cells to titanium and titanium alloy is dependent on surface roughness and composition

Cited by 639 publications

References 45 publications

Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner

Additively manufactured 3D porous Ti-6Al-4V constructs mimic trabecular bone structure and regulate osteoblast proliferation, differentiation and local factor production in a porosity and surface roughness dependent manner

Effect of Multi-nano-titania Film on Proliferation and Differentiation of Mouse Fibroblast Cell on Titanium

On the correlation between Nd:YAG laser-induced wettability characteristics modification and osteoblast cell bioactivity on a titanium alloy

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