Response of mesenchymal stem cells to surface topography of scaffolds and the underlying mechanisms

Li, Xiao; Sun, Yi; Liao, Li; Su, Xiaoxia

doi:10.1039/d2tb01875f

Cited by 12 publications

(6 citation statements)

References 179 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, studies on the biocompatibility, resistance to bacterial infection or physical stress [36], material composition for biological functions [37], surface roughness [38], modulus of elasticity [39], and surface topology/morphology [40] have been conducted to improve the function of implantable materials. In addition to these mechanical and physical functions, drug-holding and delivery capabilities are considered important parameters for predicting biological effects.…”

Section: Discussionmentioning

confidence: 99%

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

Bae,

Kim

2024

Biomed. Mater.

View full text Add to dashboard Cite

The plasma technique has been widely used to modify the surfaces of materials. The purpose of this study was to evaluate the probability of controlling the prednisolone delivery velocity on a polylactic acid (PLA) surface modified by plasma surface treatment. Surface modification of PLA was performed at a low-pressure radio frequency under conditions of 100 W power, 50 mTorr chamber pressure, 100~200 sccm of flow rate, and Ar, O2, and CH4 gases. The plasma surface-modified PLA was characterized using scanning emission microscope, X-ray photoelectron spectroscopy (XPS), and contact angle measurements. In vitro evaluations were performed to determine cellular response, drug release behavior, and anti-inflammatory effects. The PLA surface morphology was changed to a porous structure (with a depth of approximately 100 μm) and the surface roughness was also significantly increased. The XPS results demonstrated higher oxygenized carbon contents than those in the non-treated PLA group. The prednisolone holding capacity increased and the release was relatively prolonged in the surface-modified PLA group compared to that in the non-treated PLA group. In addition, cell migration and proliferation significantly increased after PLA treatment alone. The activity of cytokines such as COX-2, TNF-α, IL-1β, and IL-6 were considerably reduced in the plasma-treated and prednisolone holding group. Taken together, surface-modified PLA by plasma can provide an alternative approach to conventional physicochemical approaches for sustained anti-inflammatory drug release.

show abstract

Section: Discussionmentioning

confidence: 99%

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

Bae,

Kim

2024

Biomed. Mater.

View full text Add to dashboard Cite

show abstract

“…The morphological and other geometric characteristics of PET substrates can exert influence on the adhesion, proliferation, and differentiation of Mesenchymal Stem Cells (MSCs) on their surfaces ( Xiao et al, 2023 ). For instance, surface morphology, including features such as surface roughness and texture, can impact MSC adhesion, subsequently affecting their proliferation and differentiation ( Tang et al, 2010 ; Song et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Pulmonary decellularized extracellular matrix (dECM) modified polyethylene terephthalate three-dimensional cell carriers regulate the proliferation and paracrine activity of mesenchymal stem cells

Li,

Zhang,

et al. 2024

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Introduction: Mesenchymal stem cells (MSCs) possess a high degree of self-renewal capacity and in vitro multi-lineage differentiation potential. Decellularized materials have garnered considerable attention due to their elevated biocompatibility, reduced immunogenicity, excellent biodegradability, and the ability to partially mimic the in vivo microenvironment conducive to cell growth. To address the issue of mesenchymal stem cells losing their stem cell characteristics during two-dimensional (2D) cultivation, this study established three-dimensional cell carriers modified with lung decellularized extracellular matrix and assessed its impact on the life activities of mesenchymal stem cells.Methods: This study employed PET as a substrate material, grafting with polydopamine (PDA), and constructing a decellularized extracellular matrix (dECM) coating on its surface, thus creating the PET/PDA/dECM three-dimensional (3D) composite carrier. Subsequently, material characterization of the cellular carriers was conducted, followed by co-culturing with human umbilical cord mesenchymal stem cells in vitro, aiming to investigate the material’s impact on the proliferation and paracrine activity of mesenchymal stem cells.Results and Discussion: Material characterization demonstrated successful grafting of PDA and dECM materials, and it had complete hydrophilicity, high porosity, and excellent mechanical properties. The material was rich in various ECM proteins (collagen I, collagen IV , laminin, fibronectin, elastin), indicating good biocompatibility. In long-term in vitro cultivation (14 days) experiments, the PET/PDA/dECM three-dimensional composite carrier significantly enhanced adhesion and proliferation of human umbilical cord-derived mesenchymal stem cells (HUCMSCs), with a proliferation rate 1.9 times higher than that of cells cultured on tissue culture polystyrene (TCPS) at day 14. Furthermore, it effectively maintained the stem cell characteristics, expressing specific antigens for HUCMSCs. Through qPCR, Western blot, and ELISA experiments, the composite carrier markedly promoted the expression and secretion of key cell factors in HUCMSCs. These results demonstrate that the PET/PDA/dECM composite carrier holds great potential for scaling up MSCs’ long-term in vitro cultivation and the production of paracrine factors.

show abstract

“…Multiphoton-based 3D protein micropatterning allows human skin fibroblasts to be cultured on protein micropillar arrays with independently controllable stiffness, and it was found that cells preferentially sense the stiffness of the microenvironment rather than the elastic modulus . In addition, the surface topography of materials plays an important role in tissue engineering, for example, the morphology and differentiation of MSCs can be precisely regulated by specific scales of topography . Substrate materials of biological scaffolds can affect cell regeneration and organoid formation in tissue engineering from the surface to the inside.…”

Section: Elements Affecting Hair Cycle and Strategies To Promote Hair...mentioning

confidence: 99%

Innovative Approaches and Advances for Hair Follicle Regeneration

Zheng

2023

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Pathological hair loss (also known as alopecia) and shortage of hair follicle (HF) donors have posed an urgent requirement for HF regeneration. With the revelation of mechanisms in tissue engineering, the proliferation of HFs in vitro has achieved more promising trust for the treatments of alopecia and other skin impairments. Theoretically, HF organoids have great potential to develop into native HFs and attachments such as sweat glands after transplantation. However, since the rich extracellular matrix (ECM) deficiency, the induction characteristics of skin-derived cells gradually fade away along with their trichogenic capacity after continuous cell passaging in vitro. Therefore, ECM-mimicking support is an essential prelude before HF transplantation is implemented. This review summarizes the status of providing various epidermal and dermal cells with a threedimensional (3D) scaffold to support the cell homeostasis and better mimic in vivo environments for the sake of HF regeneration. HF-relevant cells including dermal papilla cells (DPCs), hair follicle stem cells (HFSCs), and mesenchymal stem cells (MSCs) are able to be induced to form HF organoids in the vitro culture system. The niche microenvironment simulated by different forms of biomaterial scaffold can offer the cells a network of ordered growth environment to alleviate inductivity loss and promote the expression of functional proteins. The scaffolds often play the role of ECM substrates and bring about epithelial−mesenchymal interaction (EMI) through coculture to ensure the functional preservation of HF cells during in vitro passage. Functional HF organoids can be formed either before or after transplantation into the dermis layer. Here, we review and emphasize the importance of 3D culture in HF regeneration in vitro. Finally, the latest progress in treatment trials and critical analysis of the properties and benefits of different emerging biomaterials for HF regeneration along with the main challenges and prospects of HF regenerative approaches are discussed.

show abstract

Response of mesenchymal stem cells to surface topography of scaffolds and the underlying mechanisms

Abstract: The critical role and mechanism of the topography of materials to regulate the function and fate of mesenchymal stem cells are reported.

Cited by 12 publications

References 179 publications

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

Drug release control and anti-inflammatory effect of biodegradable polymer surface modified by gas phase chemical functional reaction

Pulmonary decellularized extracellular matrix (dECM) modified polyethylene terephthalate three-dimensional cell carriers regulate the proliferation and paracrine activity of mesenchymal stem cells

Innovative Approaches and Advances for Hair Follicle Regeneration

Contact Info

Product

Resources

About