Response of mesenchymal stem cells to the biomechanical environment of the endothelium on a flexible tubular silicone substrate

O’Cearbhaill, Eoin D.; Punchard, Marie Anne; Murphy, Mary; Barry, Frank; McHugh, Peter E.; Barron, Valerie

doi:10.1016/j.biomaterials.2007.11.042

Cited by 73 publications

(52 citation statements)

References 65 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The present study suggests that shear stress induces differentiation along an endothelial lineage in canine MSCs. O'Cearbhaill et al [29] did not observe vWF expression on the protein level under static or mechanically stimulated conditions, nor did they observe a significant change in vWF at the mRNA level. The combined effect of radial stress and hoop stress used in the experiment may have surpassed the effect of shear stress in cell differentiation, because cyclic stress promotes the expression of smooth muscle-like properties [10,30,31] .…”

Section: Discussionmentioning

confidence: 91%

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

Dong

et al. 2009

Acta Pharmacol Sin

View full text Add to dashboard Cite

Aim: Recent studies have demonstrated that mesenchymal stem cells (MSCs) can differentiate into endothelial cells. The effect of shear stress on MSC differentiation is incompletely understood, and most studies have been based on two-dimensional systems. We used a model of tissue-engineered vascular grafts (TEVGs) to investigate the effects of shear stress on MSC differentiation. Methods: MSCs were isolated from canine bone marrow. The TEVG was constructed by seeding MSCs onto poly-ε-caprolactone and lactic acid (PCLA) scaffolds and subjecting them to shear stress provided by a pulsatile bioreactor for four days (two days at 1 dyne/cm 2 to 15 dyne/cm 2 and two days at 15 dyne/cm 2 ). Results: Shear stress significantly increased the expression of endothelial cell markers, such as platelet-endothelial cell adhesion molecule-1 (PECAM-1), VE-cadherin, and CD34, at both the mRNA and protein levels as compared with static control cells. Protein levels of alpha-smooth muscle actin (α-SMA) and calponin were substantially reduced in shear stresscultured cells. There was no significant change in the expression of α-SMA, smooth muscle myosin heavy chain (SMMHC) or calponin at the mRNA level. Conclusion: Shear stress upregulated the expression of endothelial cell-related markers and downregulated smooth muscle-related markers in canine MSCs. This study may serve as a basis for further investigation of the effects of shear stress on MSC differentiation in TEVGs.

show abstract

Section: Discussionmentioning

confidence: 91%

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

Dong

et al. 2009

Acta Pharmacol Sin

View full text Add to dashboard Cite

show abstract

“…Orbital shakers (or rotating disks) rotate the medium-coated disk so that the medium flows concentrically, thus generating laminar or turbulent shear stress over cells that are seeded onto the bottom of the disk (Fischer et al, 2009;Zhang et al, 2009;Bassaneze et al, 2010). Tubular shear stress systems perfuse medium through a tubular scaffold, the inner layer of which is seeded with MSCs Dong et al, 2009;O'Cearbhaill et al, 2008). Microfluidic systems contain submillimeter-sized modules that are made out of gel, such as collagen, and filled with the cells of choice; these modules are then packed into a chamber with pillars to hold them in place, before the chamber is connected to a flow circuit to undergo fluid perfusion (Bruzewicz et al, 2008;Khan and Sefton, 2010;Khan et al, 2012).…”

Section: The Effect Of Shear Stress On Endothelial Cells and Mscsmentioning

confidence: 99%

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

Dan

Velot

Decot

et al. 2015

Journal of Cell Science

View full text Add to dashboard Cite

Mesenchymal stem cells (MSCs) are among the most promising and suitable stem cell types for vascular tissue engineering. Substantial effort has been made to differentiate MSCs towards vascular cell phenotypes, including endothelial cells and smooth muscle cells (SMCs). The microenvironment of vascular cells not only contains biochemical factors that influence differentiation, but also exerts hemodynamic forces, such as shear stress and cyclic strain. Recent evidence has shown that these forces can influence the differentiation of MSCs into endothelial cells or SMCs. In this Commentary, we present the main findings in the area with the aim of summarizing the mechanisms by which shear stress and cyclic strain induce MSC differentiation. We will also discuss the interactions between these mechanical cues and other components of the microenvironment, and highlight how these insights could be used to maintain differentiation.

show abstract

“…6,[25][26][27] It has also been shown that MSC display phenotypes consistent with different perivascular cell populations and that MSC in the bone marrow reside in the bone marrow microvasculature. 28 More recently, native, noncultured perivascular cells have been shown to express MSC markers, and purified perivascular cells from skeletal muscle and nonmuscle tissues have been shown to be myogenic 3 under appropriate differentiation conditions.…”

Section: Msc Differentiation and Phenotypementioning

confidence: 99%

“…3 They are plastic adherent and can be differentiated into muscle, bone, fat, neural, cartilage, tendon, dermal, vascular, and hepatic cells. Soluble factors, 4 oxygen tension, 5 and mechanical stimuli such as shear stress 6 influence their differentiation. MSC secrete growth factors and cytokines whose effects can enhance the survival and performance of tissue engineered constructs.…”

Section: Introductionmentioning

confidence: 99%

Toward anIn VitroVasculature: Differentiation of Mesenchymal Stromal Cells Within an Endothelial Cell-Seeded Modular Construct in a Microfluidic Flow Chamber

Khan

Chamberlain

Sefton

2012

Tissue Engineering Part A

View full text Add to dashboard Cite

An in vitro tissue construct amenable to perfusion was formed by randomly packing mesenchymal stromal cell (MSC)-embedded, endothelial cell (EC)-coated collagen cylinders (modules) into a microfluidic chamber. The interstices created by the random packing of the submillimeter-sized modules created EC-lined channels. Flow caused a greater than expected amount of contraction and remodeling in the modular constructs. Flow influenced the MSC to develop smooth muscle cell markers (smooth muscle actin-positive, desmin-positive, and von Willebrand factor-negative) and migrate toward the surface of the modules. When modules were coated with EC, the extent of MSC differentiation and migration increased, suggesting that the MSC were becoming smooth muscle cell-or pericyte-like in their location and phenotype. The MSC also proliferated, resulting in a substantial increase in the number of differentiated MSC. These effects were markedly less for static controls not experiencing flow. As the MSC migrated, they created new matrix that included the deposition of proteoglycans. Collectively, these results suggest that MSC-embedded modules may be useful for the formation of functional vasculature in tissue engineered constructs. Moreover, these flow-conditioned tissue engineered constructs may be of interest as three-dimensional cell-laden platforms for drug testing and biological assays.

show abstract

Response of mesenchymal stem cells to the biomechanical environment of the endothelium on a flexible tubular silicone substrate

Cited by 73 publications

References 65 publications

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

Response of mesenchymal stem cells to shear stress in tissue-engineered vascular grafts

The role of mechanical stimuli in the vascular differentiation of mesenchymal stem cells

Toward anIn VitroVasculature: Differentiation of Mesenchymal Stromal Cells Within an Endothelial Cell-Seeded Modular Construct in a Microfluidic Flow Chamber

Contact Info

Product

Resources

About