Response of Melon Fly (Diptera: Tephritidae) to Weathered SPLAT-Spinosad-Cue-Lure

Vargas, Roger I.; Piñero, Jaime C.; Mau, Ronald F. L.; Stark, John D.; Gomez, Luis E.; Stoltman, L.; Mafra‐Neto, Agenor

doi:10.1603/ec09406

Cited by 20 publications

(17 citation statements)

References 29 publications

(16 reference statements)

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“…Weathering of the new sprayable SPLAT-MAT ME and C-L lure and kill formulations with the reduced risk insecticide spinosad was also evaluated in Hawaii (Vargas et al 2008b) and California (Vargas et al 2010b). Research has focused on SPLAT ME and C-L as replacement for Min-UGel ME and C-L, both using the toxic naled insecticide (Vargas et al 2008b(Vargas et al , 2010b.…”

Section: Discussionmentioning

confidence: 99%

“…Research has focused on SPLAT ME and C-L as replacement for Min-UGel ME and C-L, both using the toxic naled insecticide (Vargas et al 2008b(Vargas et al , 2010b. In trials conducted in California, chemical analyses of the weathered SPLAT formulation suggested a more rapid loss of ME than C-L from different dispensers (R. I.…”

Section: Discussionmentioning

confidence: 99%

“…The AWPM program successfully integrated environmentally friendly integrated pest management (IPM) technologies into a comprehensive fruit fly management package that has proven to be economically viable, environmentally compatible, and sustainable (Vargas et al 2008a) with an important level of grower adoption (Mau et al 2007). Some novel IPM technologies include applications of GF-120 NF Naturalyte protein bait as foliar sprays (Prokopy et al 2003 or in bait stations , and the use of SPLAT (Specialized Pheromone and Lure Application Technology) Male Annihilation Treatment (MAT) containing either ME or C-L (Vargas et al 2008b(Vargas et al , 2010b. Both GF-120 and SPLAT MAT-ME contain the reduced risk insecticide, spinosad, and were researched, developed and registered in Hawaii for areawide suppression of fruit flies (Mau et al 2007, Vargas et al 2008a.…”

mentioning

confidence: 99%

“…SPLAT TM is a waxy formulation of biologically inert materials used to control the release of semiochemicals with or without pesticides. Previous research in Hawaii has shown that the SPLAT matrix emits ME or C-L at effective pest suppression levels for a time interval ranging from 4-8 wk (Vargas et al , 2010b. Similarly, solid lureinsecticide dispensers have been developed and successfully evaluated for monitoring and male annihilation traps (Vargas et al 2009b(Vargas et al , 2010a allowing for the elimination of liquid lures and insecticides.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Evaluation of Cue-Lure and Methyl Eugenol Solid Lure and Insecticide Dispensers for Fruit Fly (Diptera: Tephritidae) Monitoring and Control in Tahiti

Leblanc

Vargas

Mackey³

et al. 2011

Florida Entomologist

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Cue-Lure and Methyl Eugenol Solid Lure and Insecticide Dispensers for Fruit Fly (Diptera: Tephritidae) Monitoring and Control in Tahiti

Leblanc

Vargas

Mackey³

et al. 2011

Florida Entomologist

Self Cite

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“…SPLAT-MAT is a highly adaptable product that is both amorphous and flowable (Stelinski 2007), and can be applied via a range of options, including a high-powered spray gun, regular spray equipment on a quad bike or tractor, or aerial release (Vargas et al 2008a), with unmanned devices an untested alternative. This ease of delivery may reduce labor costs, speed up delivery, and eliminate the need to establish, replenish, and retrieve devices including lure-and toxicant-impregnated blocks and wicks (Vargas et al 2009(Vargas et al , 2010Hsu and Tan 2010). Efficacy of SPLAT formulations are thought to be enhanced as several hundred point sources per hectare can be achieved via spray application, compared with the physical placement of MATs at recommended rates for male annihilation (Hsu 2010).…”

mentioning

confidence: 99%

Specialized Pheromone and Lure Application Technology as an Alternative Male Annihilation Technique to ManageBactrocera tryoni(Diptera: Tephritidae)

Reynolds¹,

Osborne²,

Crisp³

et al. 2016

J Econ Entomol

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The results of this study suggest that a novel male annihilation technique (specialized pheromone and lure application technology [SPLAT] incorporating cue-lure [CL] plus spinosad) is as effective as industry standard male annihilation controls, and is worth exploring further to manageBactrocera tryoni(Froggatt) populations. Three lures were evaluated in a contact and feeding bioassay and a cage attractancy trial: 1) SPLAT-CL + spinosad; 2) SPLAT-CL without spinosad; and 3) wick-CL + malathion. In a field attraction trial, lures (1) and (3) were evaluated with a third treatment, caneite blocks-CL + malathion. Lures were weathered for 0, 1, 2, 4, or 8 wk, with an additional weathering treatment of 12 wk included in the field trial. In the contact and feeding bioassay, lures with SPLAT-CL + spinosad were >97% effective at 48 h for up to 2 wk weathering; however, wicks-CL + malathion killedB. tryoniwithin 2 h of exposure under all weathering periods. In the cage attractancy trial, SPLAT-CL + spinosad was as effective as, or performed better than, wicks-CL + malathion under all weathering treatments. The field study trap catches were similar for SPLAT-CL + spinosad and blocks-CL + malathion, and both had higher trap catches than wicks-CL + malathion at all weathering periods, except week 12. Overall, SPLAT-CL + spinosad compared favorably with current standard techniques for male annihilation and warrants further research. SPLAT-CL + spinosad may be a reduced-risk alternative for wicks-CL + malathion or blocks-CL + malathion forB. tryoniand other CL-responding fruit flies, such asBactrocera cucurbitaeCoquillett, because it contains a reduced-risk insecticide that poses a lower risk to humans and the environment and does not require labor-intensive handling and placement.

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Embryonic microinjection of ribonucleoprotein complex (Cas9+sgRNA) of white gene in melon fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) produced white eye phenotype

Pradhan,

Karuppannasamy,

Sujatha

et al. 2023

Arch Insect Biochem Physiol

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Melon fly, Zeugodacus cucurbitae (Coquillett) is a major pest of cucurbitaceous crops, and causes substantial yield losses and economic costs. CRISPR/Cas9 is a rapid and effective site‐specific genome editing tool for the generation of genetic changes that are stable and heritable. The CRISPR/Cas9 tool uses synthetically designed single guide RNA (sgRNA) that is complementary to the target gene and guides the Cas9 enzyme to perform nuclease activity by making double‐strand breaks in the target DNA sequences. This tool can be effectively exploited to improve traits critical for the management of insect pests by targeting specific genes encoding these traits without the need of extensive genetic information. The white gene is an important gene responsible for the transport of body pigment precursor molecules. In this study, we produced effective mutagenesis of the white gene of Z. cucurbitae using the CRISPR/Cas9 tool with double sgRNA to target multiple sites of white to increase the efficiency in the generation of frame‐shift mutations resulting in the white eye phenotype in adults. This was achieved through embryonic microinjection of the ribonucleoprotein (RNP) complex in the pre‐blastoderm embryo stage 1 h after embryo laying. Our success with the production of a white eye mutant fly by CRISPR/Cas9 mutagenesis is important for the research on gene function and protein‐level modifications in melon fly and forms the basis for the development of new genetic control strategies such as precision guided sterile insect technique (pgSIT) for this pest of economic significance.

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Response of Melon Fly (Diptera: Tephritidae) to Weathered SPLAT-Spinosad-Cue-Lure

Cited by 20 publications

References 29 publications

Evaluation of Cue-Lure and Methyl Eugenol Solid Lure and Insecticide Dispensers for Fruit Fly (Diptera: Tephritidae) Monitoring and Control in Tahiti

Evaluation of Cue-Lure and Methyl Eugenol Solid Lure and Insecticide Dispensers for Fruit Fly (Diptera: Tephritidae) Monitoring and Control in Tahiti

Specialized Pheromone and Lure Application Technology as an Alternative Male Annihilation Technique to ManageBactrocera tryoni(Diptera: Tephritidae)

Embryonic microinjection of ribonucleoprotein complex (Cas9+sgRNA) of white gene in melon fly, Zeugodacus cucurbitae (Coquillett) (Diptera: Tephritidae) produced white eye phenotype

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