Response of mammalian cells to shear stress

Kretzmer, G.; Schügerl, K.

doi:10.1007/bf00167909

Cited by 83 publications

(54 citation statements)

References 6 publications

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“…22 )) have detrimental effects on cardiac cells including cell death and apoptosis. When exposed to excessive shear stress, CMs round up and show signs of dedifferentiation 5,6,[23][24][25] .…”

Section: Selecting Culture Parametersmentioning

confidence: 99%

“…Similarly, perfusion cin the range from 1 to 170 μm s −1 increased the content of DNA, glycosaminoglycans and hydroxyproline of a tissue-engineered cartilage compared to the static controls [28][29][30] . The appropriate average velocities also depend on the cell type being cultivated 23,25 . Although perfusion at 5-110 μm s −1 had beneficial effect on the constructs based on MC3T3-E1 immature osteoblasts-like cells, an average velocity of 560 μm s −1 significantly reduced the viability in the same system 31 .…”

Section: Selecting Culture Parametersmentioning

confidence: 99%

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Cardiac tissue engineering using perfusion bioreactor systems

et al. 2008

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This protocol describes tissue engineering of synchronously contractile cardiac constructs by culturing cardiac cell populations on porous scaffolds (in some cases with an array of channels) and bioreactors with perfusion of culture medium (in some cases supplemented with an oxygen carrier). The overall approach is 'biomimetic' in nature as it tends to provide in vivo-like oxygen supply to cultured cells and thereby overcome inherent limitations of diffusional transport in conventional culture systems. In order to mimic the capillary network, cells are cultured on channeled elastomer scaffolds that are perfused with culture medium that can contain oxygen carriers. The overall protocol takes 2-4 weeks, including assembly of the perfusion systems, preparation of scaffolds, cell seeding and cultivation, and on-line and end-point assessment methods. This model is well suited for a wide range of cardiac tissue engineering applications, including the use of human stem cells, and highfidelity models for biological research.

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Section: Selecting Culture Parametersmentioning

confidence: 99%

Section: Selecting Culture Parametersmentioning

confidence: 99%

Cardiac tissue engineering using perfusion bioreactor systems

et al. 2008

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“…Increasing the amount of stress or exposure time to hybridomas was found to increase damage and death rate of the cells (Kretzmer et al, 1990;Peterson et al, 1988) and increased cell detachment (Kretzmer and Schugerl, 1991). The stage of growth of CRL-8018 hybridoma cells (Peterson et al, 1988) and exposure duration and magnitude of shear stress can have an impact on the effect of shear stress on the cell.…”

Section: Introductionmentioning

confidence: 96%

Effect of shear stress on expression of a recombinant protein by Chinese hamster ovary cells

Keane

Ryan

Gray

2002

Biotech & Bioengineering

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A flow chamber was used to impart a steady laminar shear stress on a recombinant Chinese hamster ovary (CHO) cell line expressing human growth hormone (hGH). The cells were subjected to shear stress ranging from 0.005 to 0.80 N m(-2). The effect of shear stress on the cell specific glucose uptake, cell specific hGH, and lactate productivity rates were calculated. No morphological changes to the cells were observed over the range of shear stresses examined. When the cells were subjected to 0.10 N m(-2) shear in protein-free media without Pluronic F-68, recombinant protein production ceased with no change in cell morphology, whereas control cultures were expressing hGH at 0.35 microg/10(6 )cells/h. Upon addition of the shear protectants, Pluronic F-68 (0.2% [w/v]) or fetal bovine serum (1.0% [v/v] FBS), the productivity of the cells was restored. The effect of increasing shear stress on the cells in protein-free medium containing Pluronic F-68 was also investigated. Cell specific metabolic rates were calculated for cells under shear stress and for no-shear control cultures performed in parallel, with shear stress rates expressed as a percentage of those obtained for control cultures. Upon increasing shear from 0.005 to 0.80 N m(-2), the cell specific hGH productivity decreased from 100% at 0.005 N m(-2) to 49% at 0.80 N m(-2) relative to the no-shear control. A concurrent increase in the glucose uptake rate from 115% at 0.01 N m(-2) to 142% at 0.80 N m(-2), and decreased lactate productivity from 92% to 50%, revealed a change in the yield of products from glucose compared with the static control. It was shown that shear stress, at sublytic levels in medium containing Pluronic F-68, could decrease hGH specific productivity.

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“…Although at large scale this is usually achieved using stirred tanks, where the agitation is provided by an impeller and the aeration by oxygen spargers from the container bottom, 3 it was found that bursting of bubbles at the free surface 4 and excessive shear stresses 5,6 may damage the cells. These reasons kindled the interest in the use of large scale orbital shaken devices up to thousand liters.…”

Section: Introductionmentioning

confidence: 99%

Surface wave dynamics in orbital shaken cylindrical containers

et al. 2014

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Be it to aerate a glass of wine before tasting, to accelerate a chemical reaction, or to cultivate cells in suspension, the "swirling" (or orbital shaking) of a container ensures good mixing and gas exchange in an efficient and simple way. Despite being used in a large range of applications this intuitive motion is far from being understood and presents a richness of patterns and behaviors which has not yet been reported. The present research charts the evolution of the waves with the operating parameters identifying a large variety of patterns, ranging from single and multiple crested waves to breaking waves. Free surface and velocity fields measurements are compared to a potential sloshing model, highlighting the existence of various flow regimes. Our research assesses the importance of the modal response of the shaken liquids, laying the foundations for a rigorous mixing optimization of the orbital agitation in its applications. C 2014 AIP Publishing LLC. [http://dx

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Response of mammalian cells to shear stress

Abstract: The influence of shear forces on adherent mammalian cells was investigated by means of a developed flow chamber. The viability of the cells decreased with increasing exposure level and duration. Additional, changes in the morphology of the cells due to the shear forces were observed.

Cited by 83 publications

References 6 publications

Cardiac tissue engineering using perfusion bioreactor systems

Cardiac tissue engineering using perfusion bioreactor systems

Effect of shear stress on expression of a recombinant protein by Chinese hamster ovary cells

Surface wave dynamics in orbital shaken cylindrical containers

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