Response of intracellular carbohydrates to a NaCl shock in Rhizobium leguminosarum biovar trifolii TA-1 and Rhizobium meliloti SU-47

Breedveld, M.W.; Dijkema, C.; Zevenhuizen, L.P.T.M.; Zehnder, Alexander J. B.

doi:10.1099/00221287-139-12-3157

Cited by 44 publications

(36 citation statements)

References 29 publications

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“…The results from this current study showed that increasing the osmotic pressure of the growth medium increases trehalose accumulation in rhizobia which supports previously published reports that rhizobia accumulate trehalose in response to osmotic stress (Breedveld et al, 1991;Breedveld et al, 1993;Streeter, 2003;Streeter, 2007).…”

Section: General Discussion and Conclusionsupporting

confidence: 80%

“…Rhizobia synthesise endogenous trehalose when grown in a hyperosmotic environment to compensate for the difference in osmotic pressure across the cytoplasmic membrane (Streeter, 1985;Breedveld et al, 1993). There were no statistically significant differences in the trehalose content in this study, however the trehalose content was higher in TA1 cells grown in the peat extract compared to the control and was unaffected by fractionation of the peat extract.…”

Section: Discussioncontrasting

confidence: 45%

“…trifolii TA1 increased from below detection limit up to 135 µg/mg protein as osmotic pressure of the medium increased. Similarly, Breedveld et al (1993) found that the amount of intracellular trehalose in Rhizobium meliloti SU47 increased from 3 (at time zero) to 280 µg/mg protein after 80 hours of incubation in a medium containing 400 mM NaCl.…”

Section: Introductionmentioning

confidence: 98%

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The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

Casteriano¹,

Wilkes²,

Deaker³

2015

Biological Nitrogen Fixation

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Section: General Discussion and Conclusionsupporting

confidence: 80%

Section: Discussioncontrasting

confidence: 45%

Section: Introductionmentioning

confidence: 98%

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The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

Casteriano¹,

Wilkes²,

Deaker³

2015

Biological Nitrogen Fixation

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“…Under nonstress conditions, S. meliloti can take up trehalose and use it as its sole carbon and energy source (17). Under various stress conditions, S. meliloti synthesizes and accumulates trehalose, which can serve as a compatible solute (7,22). In cultures grown at high osmolarity, exogenous trehalose stimulates the accumulation of intracellular trehalose (43); this has been found to decrease the generation time (18).…”

mentioning

confidence: 99%

Redundancy in Periplasmic Binding Protein-Dependent Transport Systems for Trehalose, Sucrose, and Maltose in Sinorhizobium meliloti

2002

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We have identified a cluster of six genes involved in trehalose transport and utilization (thu) in Sinorhizobium meliloti. Four of these genes, thuE, -F, -G, and -K, were found to encode components of a binding proteindependent trehalose/maltose/sucrose ABC transporter. Their deduced gene products comprise a trehalose/ maltose-binding protein (ThuE), two integral membrane proteins (ThuF and ThuG), and an ATP-binding protein (ThuK). In addition, a putative regulatory protein (ThuR) was found divergently transcribed from the thuEFGK operon. When the thuE locus was inactivated by gene replacement, the resulting S. meliloti strain was impaired in its ability to grow on trehalose, and a significant retardation in growth was seen on maltose as well. The wild type and the thuE mutant were indistinguishable for growth on glucose and sucrose. This suggested a possible overlap in function of the thuEFGK operon with the aglEFGAK operon, which was identified as a binding protein-dependent ATP-binding transport system for sucrose, maltose, and trehalose. The K m s for trehalose transport were 8 ؎ 1 nM and 55 ؎ 5 nM in the uninduced and induced cultures, respectively. Transport and growth experiments using mutants impaired in either or both of these transport systems show that these systems form the major transport systems for trehalose, maltose, and sucrose. By using a thuE-lacZ fusion, we show that thuE is induced only by trehalose and not by cellobiose, glucose, maltopentaose, maltose, mannitol, or sucrose, suggesting that the thuEFGK system is primarily targeted toward trehalose. The aglEF-GAK operon, on the other hand, is induced primarily by sucrose and to a lesser extent by trehalose. Tests for root colonization, nodulation, and nitrogen fixation suggest that uptake of disaccharides can be critical for colonization of alfalfa roots but is not important for nodulation and nitrogen fixation per se.

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“…This has been suggested previously in studies where the growth rate of rhizobia was restricted in low-wateractivity medium and enhanced desiccation tolerance was attrib- uted to adaptation to osmotic and other stresses during growth (10,28). Rhizobia synthesize endogenous trehalose when grown in a hyperosmotic environment to compensate for the difference in osmotic pressure across the cytoplasmic membrane (23,29). In this study, while there were no statistically significant differences, the trehalose content in TA1 was higher in cells grown in the peat extract than in the control and was unaffected by fractionation of the peat extract.…”

Section: Discussionmentioning

confidence: 35%

Physiological Changes in Rhizobia after Growth in Peat Extract May Be Related to Improved Desiccation Tolerance

Casteriano

Wilkes

Deaker

2013

Appl Environ Microbiol

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Improved survival of peat-cultured rhizobia compared to survival of liquid-cultured cells has been attributed to cellular adaptations during solid-state fermentation in moist peat. We have observed improved desiccation tolerance of Rhizobium leguminosarum bv. trifolii TA1 and Bradyrhizobium japonicum CB1809 after aerobic growth in water extracts of peat. Survival of TA1 grown in crude peat extract was 18-fold greater than that of cells grown in a defined liquid medium but was diminished when cells were grown in different-sized colloidal fractions of peat extract. Survival of CB1809 was generally better when grown in crude peat extract than in the control but was not statistically significant (P > 0.05) and was strongly dependent on peat extract concentration. Accumulation of intracellular trehalose by both TA1 and CB1809 was higher after growth in peat extract than in the defined medium control. Cells grown in water extracts of peat exhibit morphological changes similar to those observed after growth in moist peat. Electron microscopy revealed thickened plasma membranes, with an electron-dense material occupying the periplasmic space in both TA1 and CB1809. Growth in peat extract also resulted in changes to polypeptide expression in both strains, and peptide analysis by liquid chromatography-mass spectrometry indicated increased expression of stress response proteins. Our results suggest that increased capacity for desiccation tolerance in rhizobia is multifactorial, involving the accumulation of trehalose together with increased expression of proteins involved in protection of the cell envelope, repair of DNA damage, oxidative stress responses, and maintenance of stability and integrity of proteins.

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Response of intracellular carbohydrates to a NaCl shock in Rhizobium leguminosarum biovar trifolii TA-1 and Rhizobium meliloti SU-47

Cited by 44 publications

References 29 publications

The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

The Physiological Mechanisms of Desiccation Tolerance in Rhizobia

Redundancy in Periplasmic Binding Protein-Dependent Transport Systems for Trehalose, Sucrose, and Maltose in Sinorhizobium meliloti

Physiological Changes in Rhizobia after Growth in Peat Extract May Be Related to Improved Desiccation Tolerance

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