Response of <i>Cucurbita</i> spp. to tomato leaf curl New Delhi virus inoculation and identification of a dominant source of resistance in <i>Cucurbita moschata</i>

Romero-Masegosa, Jonathan; Martínez, Cecilia; Aguado, Encarnación; García, Alicia; Cebrián, Gustavo; Iglesias-Moya, Jessica; Paris, Harry S.; Jamilena, Manuel

doi:10.1111/ppa.13268

Cited by 16 publications

(14 citation statements)

References 30 publications

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“…The identification of lower virus loads in C. moschata, C. ecuadorensis, and C. okeechobeensis was not surprising. Resistance to economically important viruses, including Potyvirus, Begomovirus, and Cucumovirus, has been previously identified across the three species (Brown et al, 2003;Gilbert-Albertini et al, 1993;Herrington et al, 1989;Mart ın-Hern andez and Pic o, 2021;Miranda-V elez and Wessel-Beaver, 2019;Paris and Cohen, 2000;Paris et al, 1988;Provvidenti, 1997;Robinson et al, 1988;Romero-Masegosa et al, 2020;S aez et al, 2016;Wessel-Beaver, 2005). Historically, resistance to pests and pathogens has been introduced to C. pepo through interspecific crosses (Whitaker and Robinson, 1986).…”

Section: Discussionmentioning

confidence: 99%

Field Evaluation of Cucurbita Germplasm for Resistance to Whiteflies and Whitefly-transmitted Viruses

Luckew

Meru

Wang

et al. 2022

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Summer squash (Cucurbita pepo L.) is a major vegetable crop produced in Georgia and Florida during the fall season. This production is vulnerable to whitefly (Bemisisia tabaci Genn.)-transmitted viruses that lead to severe yield losses. Over the past several years, whitefly populations have increased during the fall, thus leading to an increase in whitefly-transmitted viruses such as Cucurbit leaf crumple virus (CuLCrV) and Cucurbit yellow stunting disorder virus (CYSDV). Whitefly management for summer squash relies on the use of insecticides and can be costly without providing adequate management of the viruses. Deployment of host resistance to whiteflies and their transmitted viruses (CuLCrV and CYSDV) is the best strategy for mitigating yield loss of summer squash; however, no resistant cultivars are commercially available. In the current study, resistance or tolerance to whiteflies, CuLCrV, and CYSDV was determined for squash germplasm from the U.S. Department of Agriculture (USDA) Germplasm Resources Information Network (GRIN), university breeding programs, and commercial companies in Georgia and Florida across 2 years. In both locations and years, visual virus symptom severity scores were collected and a quantitative polymerase chain reaction (qPCR) was used to determine the CuLCrV viral load and CYSDV presence in Georgia. Whitefly-induced feeding damage was evaluated by directly assessing the intensity of silverleaf symptoms and visual counts of whitefly adults on the foliage in the field or in photographs. Virus symptom severity was lower in C. moschata Duchesne ex Poir. genotypes, namely, PI 550689, PI 550692, PI 550694, PI 653064, and Squash Betternut 900, than in other evaluated genotypes. Two C. pepo accessions were common between both locations for viral severity (PI 442294) or viral severity and viral load (PI 171625). Lower CuLCrV loads were identified in C. ecuadorensis Cutler & Whitaker (PI 540895), and C. okeechobeensis (Small) L.H.Bailey (PI 540900) than other evaluated genotypes. Four genotypes tested negative for CYSDV during both years: C. pepo (PI 507882), C. moschata (PI 483345), C. ecuadorensis (PI 390455), and C. okeechobeensis (PI 540900); they are potential sources of resistance. Six C. moschata accessions (PI 211999, PI 550690, PI 550692, PI 550694, PI 634982, and PI 653064) showed high tolerance to silverleaf disorder and had the lowest adult whitefly counts. Collectively, the accessions identified in the current study are potential sources of resistance or tolerance to whitefly and whitefly-transmitted viruses (CuLCrV and CYSDV).

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Section: Discussionmentioning

confidence: 99%

Field Evaluation of Cucurbita Germplasm for Resistance to Whiteflies and Whitefly-transmitted Viruses

Luckew

Meru

Wang

et al. 2022

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“…In Cucumis melo , resistance to ToLCNDV was found in three accessions of the momordica horticultural group and two accessions of the wild agrestis group, all from India [ 19 ]. Finally, in Cucurbita moschata , genetic resistance to ToLCNDV has been identified in five accessions from different origins, one of them from India [ 25 , 48 ]. The fact that most of the ToLCNDV-resistant cucurbit accessions come from India could be related to the co-evolution of host and pathogen in this part of the world where ToLCNDV was detected infecting cucurbits many years ago [ 49 ].…”

Section: Discussionmentioning

confidence: 99%

Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber

et al. 2021

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Tomato leaf curl New Delhi virus (ToLCNDV) is a severe threat for cucurbit production worldwide. Resistance has been reported in several crops, but at present, there are no described accessions with resistance to ToLCNDV in cucumber (Cucumis sativus). C. sativus var. sativus accessions were mechanically inoculated with ToLCNDV and screened for resistance, by scoring symptom severity, tissue printing, and PCR (conventional and quantitative). Severe symptoms and high load of viral DNA were found in plants of a nuclear collection of Spanish landraces and in accessions of C. sativus from different geographical origins. Three Indian accessions (CGN23089, CGN23423, and CGN23633) were highly resistant to the mechanical inoculation, as well as all plants of their progenies obtained by selfing. To study the inheritance of the resistance to ToLCNDV, plants of the CGN23089 accession were crossed with the susceptible accession BGV011742, and F1 hybrids were used to construct segregating populations (F2 and backcrosses), which were mechanically inoculated and evaluated for symptom development and viral load by qPCR. The analysis of the genetic control fit with a recessive monogenic inheritance model, and after genotyping with SNPs distributed along the C. sativus genome, a QTL associated with ToLCNDV resistance was identified in chromosome 2 of cucumber.

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“…In cucumber, monogenic recessive resistance to ToLCNDV has been recently identified, but preliminary mapping analyses show a different genomic region is implicated [29]. A single dominant gene conferring resistance to ToLCNDV also has been reported in a Japanese C. moschata accession, BSUAL-252 [31], though this is not linked to the genomic region on Chromosome 8 where the locus of the recessive gene for ToLCNDV resistance is located [31].…”

Section: Discussionmentioning

confidence: 99%

Resistance to Three Distinct Begomovirus Species in the Agronomical Superior Tropical Pumpkin Line AVPU1426 Developed at the World Vegetable Center

et al. 2021

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The Squash Leaf Curl China Virus (SLCCNV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) are species of Begomovirus (whitefly-vectored Geminiviridae) and cause serious damage to the cucurbit crops of the genus Cucurbita in the areas of South and Southeast Asia, across Asia, the Middle East and the Mediterranean, respectively. Cucurbita moschata’s inbred line AVPU1426, developed at the World Vegetable Center (WorldVeg) from a Bangladeshi landrace through pedigree selection, was observed to be resistant to both begomoviruses in field tests conducted at the WorldVeg Research and Training Station, Kasetsart University, Kamphaeng Saen, Thailand, which is a hotspot for these viruses. When AVPU1426 was tested for reaction to inoculation by viruliferous whiteflies with Squash Leaf Curl Philippines Virus (Taiwan strain) (SLCPV-TW) in the screen net-house at WorldVeg headquarters in Taiwan, it showed good resistance, though SLCPV-TW DNA-A could be detected in all inoculated plants, indicating that it did not show immunity to this virus. The objective of this study was to validate the resistance to SLCCNV and ToLCNDV in AVPU1426 by using whitefly-mediated inoculations to determine the mode of inheritance of the resistance. The results showed that AVPU1426 was resistant to SLCCNV and ToLCNDV. Upon crossing AVPU1426 with a susceptible check variety, Waltham Butternut, the resistance to both begomoviruses was observed to be conferred by a single recessive gene. This open-pollinated pumpkin line AVPU1426 bears flat round, mottled green immature fruits with yellow flesh in mature fruit. The fruit yield of AVPU1426 (20.74 t/ha−1) was comparable to ‘Rajah’ (18.61 t/ha−1), a recently released commercial F1 hybrid of East-West Seed (EWS). The fruit of AVPU1426 were estimated to have a good average β-carotene content (1.57 mg/100 g fresh weight). This line is a good source to breed pumpkins resistant to the three begomoviruses.

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Response of Cucurbita spp. to tomato leaf curl New Delhi virus inoculation and identification of a dominant source of resistance in Cucurbita moschata

Cited by 16 publications

References 30 publications

Field Evaluation of Cucurbita Germplasm for Resistance to Whiteflies and Whitefly-transmitted Viruses

Field Evaluation of Cucurbita Germplasm for Resistance to Whiteflies and Whitefly-transmitted Viruses

Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber

Resistance to Three Distinct Begomovirus Species in the Agronomical Superior Tropical Pumpkin Line AVPU1426 Developed at the World Vegetable Center

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