Response of human pulps capped with a self-etching adhesive system

Costa, Carlos Alberto de Souza; Nascimento, Alexandre Batista Lopes do; Teixeira, Hilcia Mezzalira; Fontana, Ueide Fernando

doi:10.1016/s0109-5641(00)00076-2

Cited by 86 publications

(48 citation statements)

References 16 publications

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“…Monocoyte/macrophage system is an important role in pulpal defense reaction particularly against physical or chemical stimuli (26,29). In this study, we determined whether lidocaine exerts toxicity in human histiocytic lymphoma U937 cells which display many monocytic characteristics and are able to differentiate to macrophages, and investigated the possible involvement of the ΔΨm and caspases in cellular toxicity.…”

Section: Western Blotting Of Poly(adp-ribose)polymerase (Parp)mentioning

confidence: 99%

Lidocaine-induced apoptosis and necrosis in U937 cells depending on its dosage

2005

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Local anesthetics are known to affect a variety of cellular responses other than the action of anesthetics through the Na + channel blockade. In this study, we examined the effect of a common local anesthetic lidocaine on the cellular activity and viability of human histiocytic lymphoma U937 cells. The cellular activity and viability were assessed by WST-1 reduction activity and trypan blue exclusion test, respectively. Induction of apoptosis was monitored by DNA ladder formation, reduction of mitochondrial transmembrane potential (ΔΨm), caspase-3 activity and nuclear morphology. Lidocaine at concentrations below 12 mM induced apoptosis characterized by DNA fragmentation and chromatin condensation dose-and time-dependently. A pan-caspase inhibitor and a caspase-3 inhibitor blocked DNA ladder formation followed by the reduction of cell death. However, the caspase inhibitors did not affect the ΔΨm, but cyclosporin A inhibited the collapse of ΔΨm followed by a reduction of cell death. Lidocaine-induced apoptosis was mitochondria-and caspase-dependent, but the collapse of ΔΨm was independent of caspase activation. At concentrations above 15 mM, lidocaine induced necrosis with early disruption of membrane integrity. These results indicate that lidocaine induced apoptosis and necrosis in U937 cells depending on its dosage.

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Section: Western Blotting Of Poly(adp-ribose)polymerase (Parp)mentioning

confidence: 99%

Lidocaine-induced apoptosis and necrosis in U937 cells depending on its dosage

2005

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“…Moreover, the hybrid layer formed on the surface of the exposed pulp may protect it from bacterial infection. Some previous studies have reported pulpal irritation caused by the chemical composition of composite resins [11][12][13][14] . However, it has been recently shown that the major cause of pulpal irritation after composite resin restoration is due to the bacterial infection or chemical irritation induced by microleakage between the cavity wall and restoration 5,6) .…”

Section: Introductionmentioning

confidence: 99%

Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping

2016

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The purpose of this study was to evaluate pulpal healing and reparative dentin formation after 14 and 28 days in exposed rat pulp directly capped with an experimentally developed all-in-one adhesive containing surface reaction-type pre-reacted glass-ionomer (S-PRG) filler. The four experimental groups and the control group were compared using the Kruskal-Wallis test, followed by the Steel-Dwass post-hoc test to compare the histopathological score. The Mann-Whitney U test was used to compare the histopathological score at 14 and 28 days for each observation item. All experimental adhesives containing S-PRG fillers developed for direct pulp capping showed no pulpal inflammation. After 14 days, the experimental adhesives containing S-PRG fillers and the control group formed tertiary dentin around the exposed pulp. After 28 days, the experimental adhesives containing 13 and 27 wt% of S-PRG fillers formed dentin bridge equal to the control.

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“…However, in the literature, including several histologybased reports of direct pulp-capping for mechanically exposed human teeth, the situation is different. Bonding agents such as All Bond 2 (Bisco) 127) , Clearfil Liner Bond 2 (Kuraray, Tokyo, Japan) 128) , Scotch Bond MultiPurpose (3M ESPE) 129) , Single Bond (3M ESPE) 130) and composite resin (Z100) (3M ESPE) were applied to the pulp for periods ranging from 2 to 10 months. Calciumhydroxide-based cement (Dycal) was used as a control in all cases.…”

Section: Calciummentioning

confidence: 99%

Current status of direct pulp-capping materials for permanent teeth

Komabayashi

Zhu

Eberhart

et al. 2016

Dental Materials Journal

122

125

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Direct pulp-capping is a method for treating exposed vital pulp with dental material to facilitate the formation of reparative dentin and to maintain vital pulp. Two types of pulp-capping materials, calcium hydroxide and mineral trioxide aggregate, have been most commonly used in clinics, and an adhesive resin has been considered a promising capping material. However, until now, there has been no comprehensive review of these materials. Therefore, in this paper, the composition, working mechanisms and clinical outcome of these types of pulp-capping materials are reviewed.

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Response of human pulps capped with a self-etching adhesive system

Cited by 86 publications

References 16 publications

Lidocaine-induced apoptosis and necrosis in U937 cells depending on its dosage

Lidocaine-induced apoptosis and necrosis in U937 cells depending on its dosage

Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping

Current status of direct pulp-capping materials for permanent teeth

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