Response of human oral epithelial cells to oxidative damage and the effect of vitamin E

Royack, Greg A.; Nguyen, Michelle; Tong, D. M.; Poot, Martin; Oda, Dolphine

doi:10.1016/s1368-8375(99)00047-0

Cited by 33 publications

(26 citation statements)

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“…Excess ROS or non-functionality of these enzymes may induce toxicity, mutations and ultimately cancer in various human tissues. 3,4 Detoxification enzymes play variable roles in oral cancer development as implied from several studies. Expression of mRNA for various CYPs 5,6 and metabolism of the polycyclic aromatic hydrocarbon benzo(a)pyrene to DNA binding intermediates 7 in cultured human NOK indicate existence of oxidative metabolism.…”

mentioning

confidence: 99%

Transcript profiling of enzymes involved in detoxification of xenobiotics and reactive oxygen in human normal and simian virus 40 T antigen‐immortalized oral keratinocytes

Vondracek

Weaver

Sarang

et al. 2002

Intl Journal of Cancer

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The metabolic detoxification capacity may critically regulate the susceptibility of human tissues to cancer development. We used standardized and quantitative, reverse transcription-polymerase chain reaction (StaRT-PCR) and microarray chip techniques to analyze transcript levels of multiple detoxification enzymes in cultured normal human oral keratinocytes (NOK) and the Siman virus 40 T antigenimmortalized oral keratinocyte line SVpgC2a, viewing the latter as a model of a benign tumor state. With good agreement between the 2 methodologies, NOK and SVpgC2a were found to express transcripts for cytochrome P450 enzymes (CYPs), factors related to CYP induction and enzymes involved in conjugation reactions or detoxification of reactive oxygen. The cell types expressed similar levels of CYP 2B6/7, CYP 2E1, P450 oxidoreductase, the aryl hydrocarbon receptor nuclear translocator, sulfotransferase 1A1, sulfotransferase 1A3, epoxide hydrolase, glutathione S-transferase M3, glutathione S-transferase pi and catalase, superoxide dismutase 1, glutathione peroxidase 1 and glutathione peroxidase 3. In contrast, SVpgC2a exhibited comparatively higher levels of CYP1A1, 1B1, aryl hydrocarbon receptor, glutathione S-transferase M1, 2, 4, 5, glutathione S-transferase theta 1 and superoxide dismutase 2 and comparatively lower levels of UDP glycosyltransferase 2 and microsomal glutathione S-transferase 1. Some transcripts, e.g., CYP 2A6/7, were not detected by either standard, non quantitative RT-PCR or the above methods, whereas others were barely quantifiable by StaRT-PCR, i.e., were present at 1-10 molecules/10 6 molecules of actin. Overall, the expression analysis demonstrated presence of mRNA for multiple enzymes involved in foreign compound metabolism and detoxification pathways, including several enzymes not previously reported for oral epithelium. Generally, the comparison of NOK from 2 individuals indicated relatively similar transcript levels of these enzymes. In contrast, differences between NOK and SVpgC2a, e.g., for CYP1B1, may reflect alteration caused by immortalization and aid identification of early stage tumor markers in oral epithelium. © 2002 Wiley-Liss, Inc. Key words: oral epithelium; biotransformation; phase I and II metabolizing enzymes; mRNA expressionSeveral enzyme systems including cytochrome P450s (CYPs), conjugation enzymes and enzymes involved in detoxification of reactive oxygen species (ROS) cooperate to protect the organism against reactive agents that are potentially carcinogenic and stress inducing. 1 Oxidative metabolism (so called phase I metabolism), carried out by the CYP family of enzymes, initiate biotransformation of compounds, which do not possess functional groups suitable for conjugation and can thereby generate toxic and mutagenic/ carcinogenic intermediates. 2 Following CYP dependent oxidative activation, conjugation enzymes (involved in so called phase II metabolism) including transferases for glutathione, sulfate, acetyl moieties and glucuronic acid generally decrease the reactivity of ...

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confidence: 99%

Transcript profiling of enzymes involved in detoxification of xenobiotics and reactive oxygen in human normal and simian virus 40 T antigen‐immortalized oral keratinocytes

Vondracek

Weaver

Sarang

et al. 2002

Intl Journal of Cancer

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“…These observations too point towards the antioxidant properties of AT. Royack et al [10] have also reported that vitamin E as such may have the potential to reduce oxidative damage caused by hydroxyl free radicals.…”

Section: Discussionmentioning

confidence: 99%

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2001

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“…From this plant are obtained for various purposes such as therapeutic drugs effective cicatrizing and antiinflammatory. This action contributes to improving the general conditions of the gingival epithelium, which reduces inflammation and bleeding, characteristic signs of periodontal disease in patients with xerostomia [78], [119], [120]. For xerostomic patients with oral candidiasis is indicated the use of topical antifungal agents such as miconazole or nistanina three times a day for 15 days.…”

Section: Treatmentmentioning

confidence: 99%

The use of drugs for the treatment of xerostomia – brief review

César

Justo

Vidal

et al. 2016

IJBAS

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Inadequate production of saliva causes an oral imbalance that has a great impact on the individual's quality of life. Diseases of various types are responsible for impaired production of saliva and dry mouth, as happens in xerostomia. It is usually associated with hypofunction of the salivary glands, which shows signs of dryness on oral mucosa, and other morphological features in the oral cavity. Saliva is composed of water and 1% of electrolytes and immunoglobulins, enzymes and other proteins. Its fluid component, which contains ions, is mainly produced by parasympathetic stimulation, while the protein component, produced in the acinar secretory vesicles, is released by sympathetic stimulation. The use of certain drugs is a major cause of xerostomia. Among these we found analgesics, anticonvulsants, antihistamines, antihypertensives, diuretics and antidepressants. The treatment is mainly done by saliva substitutes such as sodium chloride, potassium chloride, monopotassium phosphate and dipotassium phosphate. However, in some cases, stimulants, such as citric acid and sodium citrate, or even topical salivary substitutes can be effective. Furthermore, the use of specific oral care products is well accepted by the patients. The treatment is selected according to the ability of the glands to produce saliva. It is still not fully known what mechanism of action drugs that cause hyposalivation, dry mouth or changes in saliva composition use. Yet, it is important to know the side effects of prescribed drugs.

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Response of human oral epithelial cells to oxidative damage and the effect of vitamin E

Cited by 33 publications

References 14 publications

Transcript profiling of enzymes involved in detoxification of xenobiotics and reactive oxygen in human normal and simian virus 40 T antigen‐immortalized oral keratinocytes

Transcript profiling of enzymes involved in detoxification of xenobiotics and reactive oxygen in human normal and simian virus 40 T antigen‐immortalized oral keratinocytes

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The use of drugs for the treatment of xerostomia – brief review

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