Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration

Hu, Hanhua; Gao, Kunshan

doi:10.1007/s10529-006-9026-6

Cited by 167 publications

(93 citation statements)

References 15 publications

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“…grown under elevated salinity. A lower degree of unsaturation of lipids at elevated NaCl concentrations was reported for the algae Dunaliella sp., Nannochloropsis sp., and N. frustulum (Renaud and Parry 1994;Xu and Beardall 1997;Hu and Gao 2006).…”

Section: Discussionmentioning

confidence: 99%

Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kütz IPPAS H-252

2010

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The effect of 0.3 and 0.7 M NaCl on biomass yield, total nitrogen content, intracellular lipid content, and fatty acid profile of the lipids of the alga Botryococcus braunii IPPAS H-252 in different phases of the culture cycle was studied. The presence of sodium chloride in the medium inhibited the growth of algal cells for the first 3 days of the experiment, causing a decrease in total nitrogen, enhanced synthesis of triacylglycerols, and considerable changes in the lipid fatty acid profile: decreases in polyenoic acid contents (from 68.34% to 29.38% and 12.8%) and proportions of long-chain saturated acids (from 0.53% to 5.3% and 14.13% of the total fatty acids) at 0.3 M NaCl and 0.7 M NaCl, respectively. In later phases of the culture, at 0.3 M NaCl, the content of polyenoic acids rose to the values characteristic of the active growth phase of this alga. At 0.7 M NaCl, the proportion of polyenoic acids grew less significantly, but biomass concentration and total nitrogen increased, similarly to the experiment with 0.3 M NaCl.

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Section: Discussionmentioning

confidence: 99%

Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kütz IPPAS H-252

2010

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“…Also, overproduction of neutral lipids in a mutant of C. reinhardtii under nitrogen starvation has been achieved through blocking a competing pathway to produce starch . For Nannochloropsis spp., it has been reported that nitrogen shortage can lead to significant accumulation of saturated and monounsaturated fatty acids (Hu and Gao, 2006). These fatty acids are mainly associated with storage triacylglycerols, the preferred substrate for biodiesel production.…”

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confidence: 99%

Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

et al. 2013

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The Nannochloropsis genus contains oleaginous microalgae that have served as model systems for developing renewable biodiesel. Recent genomic and transcriptomic studies on Nannochloropsis species have provided insights into the regulation of lipid production in response to nitrogen stress. Previous studies have focused on the responses of Nannochloropsis species to short-term nitrogen stress, but the effect of long-term nitrogen deprivation remains largely unknown. In this study, physiological and proteomic approaches were combined to understand the mechanisms by which Nannochloropsis oceanica IMET1 is able to endure long-term nitrate deprivation and its ability to recover homeostasis when nitrogen is amended. Changes of the proteome during chronic nitrogen starvation espoused the physiological changes observed, and there was a general trend toward recycling nitrogen and storage of lipids. This was evidenced by a global down-regulation of protein expression, a retained expression of proteins involved in glycolysis and the synthesis of fatty acids, as well as an up-regulation of enzymes used in nitrogen scavenging and protein turnover. Also, lipid accumulation and autophagy of plastids may play a key role in maintaining cell vitality. Following the addition of nitrogen, there were proteomic changes and metabolic changes observed within 24 h, which resulted in a return of the culture to steady state within 4 d. These results demonstrate the ability of N. oceanica IMET1 to recover from long periods of nitrate deprivation without apparent detriment to the culture and provide proteomic markers for genetic modification.

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“…8,9) In recent years, various strains of Nannochloropsis have been investigated for their biomass production, lipid composition, and content under different growth conditions. 8,[10][11][12][13][14] To further develop them into an integrated photons-to-fuel production platform, high-efficiency transformation methods are necessary.…”

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confidence: 99%

High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product

Gao

2014

Bioscience, Biotechnology, and Biochemistry

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Nannochloropsis are model species for investigating biofuel production by algae. To develop them into an integrated photons-to-fuel production platform, high efficiency transformation methods are necessary. Here, we obtained the β-tubulin promoter regions of all recognized species of genus Nannochloropsis, and successfully transformed all five marine species by electroporation. In addition, the PCR amplified double stranded DNA fragments (PCR fragments) based transformation system was established in these Nannochloropsis species, which showed much higher transformation efficiency (10.7–61.2 × 10−6, 1.5–13-fold) than that of linearized plasmid based transformation. The cotransformation of N. salina using a circular plasmid containing a non-selectable GUS gene and a PCR fragment containing only a selection marker cassette was also achieved and found to be very efficient (over 50%). This simple and highly efficient transformation protocol reported in our study provided a useful tool for gene functional analysis and genetic engineering of the oleaginous Nannochloropsis species.

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Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration

Cited by 167 publications

References 15 publications

Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kütz IPPAS H-252

Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kütz IPPAS H-252

Responses of Nannochloropsis oceanica IMET1 to Long-Term Nitrogen Starvation and Recovery

High-efficiency nuclear transformation of the oleaginous marine Nannochloropsis species using PCR product

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