Response of foot-and-mouth disease virus C3 Resende to immunological pressure exerted in vitro by antiviral polyclonal sera

Schiappacassi, Mónica; Rojas, Elizabeth Rieder; Carrillo, Elisa; Campos, Rodolfo Héctor

doi:10.1016/0168-1702(94)00099-x

Cited by 14 publications

(10 citation statements)

References 35 publications

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“…This extreme heterogeneity of RNA virus has also been reported among clones from single isolates of foot-and-mouth disease viruses (Domingo et al 1980, Schiappacassi et al 1995. This is thought to be due to an inefficient nucleic acid repair system that retains transcription errors at a high rate compared to those in DNA virus.…”

Section: Discussionmentioning

confidence: 87%

“…Upper triangle indicates percent similarity; lower triangle indicates percent divergence. Ja = Jasper-ATCC, Ja-D = Jasper-Dobos, Ja-R = Jasper isolate which is resistant to RTS, Ja-S = Jasper isolate which is sensitive to RTS (Schiappacassi et al 1995). It has been reported that IPNV is highly heterogeneous at the genome level , 1993, Zhang & Suzuki 2003.…”

Section: Discussionmentioning

confidence: 99%

“…Schiappacassi et al (1995) reported polyclonal antibody neutralizationresistant (Nr) variants from a population of footand-mouth disease virus C 3 Resende strain. Resende original strain (C 0 ) was passaged 25 times under immunological pressure (F 1 , F 2 , F 3 , F 4 and F 5 ) or under non-immune pressure as a control (C 1 , C 2 , C 3 ).…”

Section: Discussionmentioning

confidence: 99%

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Normal rainbow trout serum (RTS)-resistant variants of the infectious pancreatic necrosis virus (IPNV)-Jasper differ with respect to inhibition by RTS, serotype and cDNA sequence

Park¹,

Lee²,

Reno³

2004

Dis. Aquat. Org.

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In order to determine if the infectious pancreatic necrosis virus isolate IPNV-Jasper (Ja-ATCC) is homogeneous or heterogeneous with respect to inhibition by normal rainbow trout serum (RTS), 50 clones were tested for sensitivity to RTS. The initial isolate was very sensitive to RTS, losing from 10 4 to 10 8 50% tissue culture infection dose (TCID 50 ) ml -1 with a 1:100 dilution of RTS. The sensitivity of the clones ranged from highly sensitive to completely resistant (0 to 10 8 TCID 50 ml -1 reduction). Eight percent of clones (4/50) were very sensitive to RTS (Ja-S) and 84% of clones (42/50) showed a mid-range of sensitivity to RTS. The final 8% of clones (4/50) were resistant to RTS (Ja-R). Enzyme immunodot assay revealed that Ja-S clones showed a monoclonal reaction identical to the parents, Ja-ATCC; however, Ja-R clones differed by several epitopes from the parental strain. Analysis of Ja-S and Ja-R revealed that there were significant differences in their nucleic acid sequences for the capsid protein VP2. These 2 strains shared 80.7 and 86.5% identity in nucleic acid and in amino acid sequences, respectively. Ja-S had 99.7 and 91.0% identity in nucleic acid sequences, and 99.5 and 95.9% in amino acid sequences with Ja-ATCC and Jasper-Dobos (Ja-D), respectively, while Ja-R showed 80.6 and 79.8% identity in nucleic acid sequences and 86.5 and 87.0% in amino acid sequences with Ja-ATCC and Ja-D, respectively. In conclusion, the Ja-ATCC population was heterogeneous in terms of RTS sensitivity, serotype and cDNA sequences from the VP2 coding region. KEY WORDS: IPNV-Jasper variants · RTS inhibition · Serotype · cDNA sequenceResale or republication not permitted without written consent of the publisher

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Section: Discussionmentioning

confidence: 87%

Section: Discussionmentioning

confidence: 99%

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Normal rainbow trout serum (RTS)-resistant variants of the infectious pancreatic necrosis virus (IPNV)-Jasper differ with respect to inhibition by RTS, serotype and cDNA sequence

Park¹,

Lee²,

Reno³

2004

Dis. Aquat. Org.

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“…Cultured-adapted Long strain virus (3.6 9 10 6 PFU/ mL) was mixed (1:1) with subneutralizing dilutions of the anti-F serum in order to select the optimal working dilution to avoid complete pressure of the virus, which might alter protein function [32,33]. We selected 1/500 as our starting working dilution.…”

Section: Mutant Selectionmentioning

confidence: 99%

Selection and characterization of human respiratory syncytial virus escape mutants resistant to a polyclonal antiserum raised against the F protein

et al. 2012

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“…Many strategies have been used to produce FMD vaccines which do not require large scale growth of the infectious virus. These strategies have included various peptide vaccines and DNA vaccines using the capsid protein, VP1, flanking amino acid residues 21À40, 135À160, and 200À213 (Parry et al 1990, Schiappacassi et al 1995, Blanco et al 2001. However, the synthetic or recombinant products have a limited number of Tcell epitopes of viral immunogens to present to the vaccinated animals and, although they often induce production of high titres of neutralizing antibodies, the neutralizing antibodies are not always protective in livestock.…”

Section: Introductionmentioning

confidence: 98%

A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

Song

Wang²,

Zheng³

et al. 2005

Biotechnol Lett

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Epitopes of a foot-and-mouth disease virus (FMDV) capsid protein VP1 complex and a chimera of 6xHis-tagged cholera toxin B subunit (hCTB) were expressed in Hansenula polymorpha and used together as a mucosal vaccine. Antibody and cytokine responses to VP1-hCTB vaccine and protection against FMDV were evaluated by ELISA and a virus challenge test in mice, respectively. VP1-hCTB directly enhanced the expression of interleukin-5 (IL-5) both in serum and supernatants of cultured spleen cells. After challenging suckling mice with 10(5) FMDV (=50% lethal dosage per mouse) a greater protection was seen after intraperitoneal and intranasal vaccinations than after oral vaccination. In swine immunized with VP1-hCTB, immune responses were achieved after three administrations, and the vaccine protected swine (80%) when challenged with 10(6.5) FMDV (=50% infectious dosage per swine). These results demonstrated the possibility of using CTB as a mucosal adjuvant to elicit protective immune responses against FMDV.

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Response of foot-and-mouth disease virus C3 Resende to immunological pressure exerted in vitro by antiviral polyclonal sera

Cited by 14 publications

References 35 publications

Normal rainbow trout serum (RTS)-resistant variants of the infectious pancreatic necrosis virus (IPNV)-Jasper differ with respect to inhibition by RTS, serotype and cDNA sequence

Normal rainbow trout serum (RTS)-resistant variants of the infectious pancreatic necrosis virus (IPNV)-Jasper differ with respect to inhibition by RTS, serotype and cDNA sequence

Selection and characterization of human respiratory syncytial virus escape mutants resistant to a polyclonal antiserum raised against the F protein

A Novel Mucosal Vaccine Against Foot-and-Mouth Disease Virus Induces Protection in Mice and Swine

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