Response of extrapancreatic glucagon to arginine in dogs.

Effect of insulin treatment upon response of extrapancreatic glucagon to arginine.

“…However, it was demonstrated in the previous study that extrapancreatic glucagon in the portal vein increased at fasting and rose markedly in response to arginine infusion performed 1 week after total pancreatectomy in dogs, which were devoid of insulin as well as pancreatic glucagon (Ohneda et al 1979). In contrast, arginine infusion performed after pancreatectomy resulted in an increase of extrapancreatic glucagon moderately in the alloxan diabetic dogs, in which insulin deficiency was clearly demonstrated.…”

“…In order to investigate the response of extrapancreatic glucagon, arginine was admin istered into the femoral vein before and after total pancreatectomy in 5 normal dogs and 14 alloxan diabetic dogs with or without insulin treatment , as described previously (Ohneda et al 1979). In 14 dogs, alloxan was injected intravenously a week before the experiment and 6 dogs of them were treated with lente insulin in a daily dose of 4 to 10 units , as mentioned above.…”

“…In addition, plasma immunoreactive glucagon was measur ed with an antiserum (G25), which cross-reacts with both pancreatic glucagon and gut glucagon-like immunoreactive substance, and was designated as total immunoreactive glucagon (IRG). The results for the normal dogs in the anesthetized experiment were quoted from the previous study (Ohneda et al 1979). Comparison of responses of plasma glucagon and total IRG to arginine.…”

Response of extrapancreatic glucagon to glycemic changes under chronic insulin deficiency in dogs.

“…In dogs, the stomach is known as a main origin of extrapancreatic glucagon (Blazquez et al 1976;Ohneda et al 1979). According to the histological studies (Sasaki et al 1975;Orci and Perrelet 1981), glucagon-secreting cells in the gastrointestinal tract are scattered diffusely in the epithelium, unlike A cells in the pancreatic islet, which are closely packed with B and D cells and receive innervation.…”

Response of extrapancreatic glucagon to gastrointestinal hormones in pancreatectomized dogs.

“…The present study was carried out using 24 healthy mongrel dogs weighing 15 to 23 kg. After an overnight fast, the abdomen was opened by a midline incision under anesthesia with sodium pentobarbital and the pancreas was carefully removed together with a part of the duodenum (Ohneda et al 1979). A polyethylene catheter was inserted into the portal vein through a mesenteric vein and anchored so that the tip of the catheter reached just below the hepatic hilus.…”