Response of extracellular and intracellular alkaline phosphatase in Microcystis aeruginosa to organic phosphorus

Zhang, Tingxi; Lu, Xiaoran; Yu, Rongda; Mengyao, Qin; Wei, Chao; Hong, Sujuan

doi:10.1007/s11356-020-09736-7

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…Among the dissolved P sources, only orthophosphates can be directly adsorbed onto and transported through the cell membrane, whereas OP must be converted into orthophosphates via hydrolysis by APase before adsorption. 9,10,12 Here, the TP concentration decreased more with the IP than with the OP in ). Increasing the APase activity is a widely adopted method to enhance the bioavailability of environmental P for phytoplankton, including Microcystis.…”

Section: Effects Of Brucella Sp On the Solubilization Of Limited Solu...mentioning

confidence: 79%

“…Previous studies similarly showed an increase in the extracellular APase, followed by a decreased or constant APase content in Microcystis when using several OP sources (e.g., adenosine triphosphate and β-glycerophosphate), which is due to a continuous OP consumption rather than the inhibition of the related gene expression by PO 4 . [3][4][5][7][8][9][10]14,60,61 The changes in TP and concentrations of extracellular APase attributed to Brucella sp. were relatively smaller than those attributed to Microcystis in the cocultures (Figures S4 and S5), indicating that the majority of P sources were converted and utilized by Microcystis, with a minor contribution from Brucella sp.…”

Section: Effects Of Brucella Sp On the Solubilization Of Limited Solu...mentioning

confidence: 99%

“…8−11 The bioavailability of IP is higher than that of OP. 9,10,12 The reduced bioavailability of OP leads to a significant decrease in the M. aeruginosa growth rate and biomass. The maximum biomass of M. aeruginosa in an OP medium (i.e., containing β-glycerophosphate) was reportedly nearly 10 times lower than that in an IP medium (i.e., containing dipotassium hydrogen phosphate) at the same P concentration.…”

Section: Introductionmentioning

confidence: 99%

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Roles of Attached Bacteria on the Growth and Metabolism of Microcystis aeruginosa with Limited Soluble Phosphorus

Song,

Li,

Zou

et al. 2024

ACS EST Water

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The limited endogenous soluble phosphorus (P) in lake sediment is a potential and non-negligible P pool for the growth of Microcystis species and the bacteria attached to them. The attached bacteria are considered as P utilization supporters that contribute to Microcystis blooms; however, their effects on Microcystis growth and metabolism during the utilization of limited soluble P are poorly understood. Here, a series of cocultivations of Microcystis aeruginosa and a Microcystis growth-promoting bacterium, Brucella sp., were conducted using two P sources with limited solubility (tricalcium phosphate and calcium phytate). The results indicated that Brucella sp. secreted auxins to directly promote the growth of M. aeruginosa, and the secreted organic acids and alkaline phosphatase accelerated the overall P solubilization and transformation. In addition, the coexistence of M. aeruginosa with Brucella sp., which resulted in competition for P, stimulated M. aeruginosa to synthesize more photosynthetic pigments and alkaline phosphatase. Therefore, the results revealed that the attached bacteria improved the properties of M. aeruginosa and the phycosphere (e.g., the availability of P sources), providing comprehensive insights into their contribution to endogenous P utilization by Microcystis and the further occurrence of cyanobacterial blooms.

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Section: Effects Of Brucella Sp On the Solubilization Of Limited Solu...mentioning

confidence: 79%

Section: Effects Of Brucella Sp On the Solubilization Of Limited Solu...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Roles of Attached Bacteria on the Growth and Metabolism of Microcystis aeruginosa with Limited Soluble Phosphorus

Song,

Li,

Zou

et al. 2024

ACS EST Water

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“…This activation destabilized phoU and led to the phosphorylation of phoR - phoB , a two-component system extensively studied in E.coli under P i starvation . The activated phoR - phoB operon initiated the transcription of APase (e.g., encoded by phoA , phoX , and phoD in prokaryotes), which plays a vital role in mineralizing dissolved organic P into bioavailable P i to promote cyanobacterial uptake. ,, The imported P i was utilized by bacteria and actively synthesized into polyP with ppk ( ppk1 and ppk2 ) as reservoirs of P i . P i was released from polyP by ppx , serving as an energy source. , The stringent alarmone molecule (p)ppGpp was generated through the interaction between GDP (or GTP) and ATP, mediated by the relA in response to amino acid starvation and spoT under various stress (e.g., carbon and P i starvation) …”

Section: Resultsmentioning

confidence: 99%

“…69 The activated phoR-phoB operon initiated the transcription of APase (e.g., encoded by phoA, phoX, and phoD in prokaryotes), which plays a vital role in mineralizing dissolved organic P into bioavailable P i to promote cyanobacterial uptake. 8,70,71 The imported P i was utilized by bacteria and actively synthesized into polyP with ppk (ppk1 and ppk2) as reservoirs of P i . P i was released from polyP by ppx, serving as an energy source.…”

Section: Schematic Model Of Stringentmentioning

confidence: 99%

Stringent Response of Cyanobacteria and Other Bacterioplankton during Different Stages of a Harmful Cyanobacterial Bloom

Li,

Bhattarai,

Barber

et al. 2023

Environ. Sci. Technol.

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We conducted a field study to investigate the role of stringent response in cyanobacteria and coexisting bacterioplankton during nutrient-deprived periods at various stages of bloom in a freshwater lake (Utah Lake) for the first time. Using metagenomics and metatranscriptomics analyses, we examined the cyanobacterial ecology and expression of important functional genes related to stringent response, N and P metabolism, and regulation. Our findings mark a significant advancement in understanding the mechanisms by which toxic cyanobacteria survive and proliferate during nitrogen (N) and phosphorus (P) limitations. We successfully identified and analyzed the metagenome-assembled genomes (MAGs) of the dominant bloom-forming cyanobacteria, namely, Dolichospermum circinale, Aphanizomenon flos-aquae UKL13-PB, Planktothrix agardhii, and Microcystis aeruginosa. By mapping RNA-seq data to the coding sequences of the MAGs, we observed that these four prevalent cyanobacteria species activated multiple functions to adapt to the depletion of inorganic nutrients. During and after the blooms, the four dominant cyanobacteria species expressed high levels of transcripts related to toxin production, such as microcystins (mcy), anatoxins (ana), and cylindrospermopsins (cyr). Additionally, genes associated with polyphosphate (poly-P) storage and the stringent response alarmone (p)ppGpp synthesis/hydrolysis, including ppk, relA, and spoT, were highly activated in both cyanobacteria and bacterioplankton. Under N deficiency, the main N pathways shifted from denitrification and dissimilatory nitrate reduction in bacterioplankton toward N2-fixing and assimilatory nitrate reduction in certain cyanobacteria with a corresponding shift in the community composition. P deprivation triggered a stringent response mediated by spoT-dependent (p)ppGpp accumulation and activation of the Pho regulon in both cyanobacteria and bacterioplankton, facilitating inorganic and organic P uptake. The dominant cyanobacterial MAGs exhibited the presence of multiple alkaline phosphatase (APase) transcripts (e.g., phoA in Dolichospermum, phoX in Planktothrix, and Microcystis), suggesting their ability to synthesize and release APase enzymes to convert ambient organic P into bioavailable forms. Conversely, transcripts associated with bacterioplankton-dominated pathways like denitrification were low and did not align with the occurrence of intense cyanoHABs. The strong correlations observed among N, P, stringent response metabolisms and the succession of blooms caused by dominant cyanobacterial species provide evidence that the stringent response, induced by nutrient limitation, may activate unique N and P functions in toxin-producing cyanobacteria, thereby sustaining cyanoHABs.

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Cyanobacterial extracellular alkaline phosphatase: detection and ecological function

et al. 2022

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Response of extracellular and intracellular alkaline phosphatase in Microcystis aeruginosa to organic phosphorus

Cited by 15 publications

References 34 publications

Roles of Attached Bacteria on the Growth and Metabolism of Microcystis aeruginosa with Limited Soluble Phosphorus

Roles of Attached Bacteria on the Growth and Metabolism of Microcystis aeruginosa with Limited Soluble Phosphorus

Stringent Response of Cyanobacteria and Other Bacterioplankton during Different Stages of a Harmful Cyanobacterial Bloom

Cyanobacterial extracellular alkaline phosphatase: detection and ecological function

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