Response of endophytic bacteria in sugarcane roots to different slow-release fertilizers with dicyandiamide (DCD) and humic acid (HA) applications

Xiao, Jian; Chen, Siyu; Liang, Tian; Yang, Shangdong; Tan, Huachun

doi:10.1016/j.eti.2023.103244

Cited by 5 publications

(7 citation statements)

References 49 publications

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“…Additionally, the introduction of HA was found to increase the net photosynthetic rate (Pn) and decrease the transpiration rate of wheat under drought stress . Furthermore, HA has the ability to improve nutrient uptake by stimulating root growth and also plays a functional role in protecting plants from oxidative stress …”

Section: Resultsmentioning

confidence: 99%

Humic Acid-Functionalized Lignin-Based Coatings Regulate Nutrient Release and Promote Wheat Productivity and Grain Quality

El Bouchtaoui,

Ablouh,

Mhada

et al. 2024

ACS Appl. Mater. Interfaces

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The rational application of fertilizers is crucial for achieving high crop yields and ensuring global food security. The use of biopolymers for slow-release fertilizers (SRFs) development has emerged as a game-changer and environmentally sustainable pathway to enhance crop yields by optimizing plant growth phases. Herein, with a renewed focus on circular bioeconomy, a novel functionalized lignin-based coating material (FLGe) was developed for the sustained release of nutrients. This innovative approach involved the extraction and sustainable functionalization of lignin through a solvent-free esterification reaction with humic acid�an organic compound widely recognized for its biostimulant properties in agriculture. The primary objective was to fortify the hydration barrier of lignin by reducing the number of its free hydroxyl groups, thereby enhancing release control, while simultaneously harnessing the agronomic benefits offered by humic acid. After confirming the synthesis of functionalized lignin (FLGe) through 13 C NMR analysis, it was integrated at varying proportions into either a cellulosic or starch matrix. This resulted in the creation of five distinct formulations, which were then utilized as coatings for diammonium phosphate (DAP) fertilizer. Experimental findings revealed an improved morphology and hardness (almost 3-fold) of DAP fertilizer granules after coating along with a positive impact on the soil's water retention capacity (7%). Nutrient leaching in soil was monitored for 100 days and a substantial reduction of nutrients leaching up to 80% was successfully achieved using coated DAP fertilizer. Furthermore, to get a fuller picture of their efficiency, a pot trial was performed using two different soil textures and demonstrated that the application of FLGe-based SRFs significantly enhanced the physiological and agronomic parameters of wheat, including leaf evolution and root architecture, resulting in an almost 50% increase in grain yield and improved quality. The results proved the potential of lignin functionalization to advance agricultural sustainability and foster a robust bioeconomy aligning with the premise "from the soil to the soil".

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Section: Resultsmentioning

confidence: 99%

Humic Acid-Functionalized Lignin-Based Coatings Regulate Nutrient Release and Promote Wheat Productivity and Grain Quality

El Bouchtaoui,

Ablouh,

Mhada

et al. 2024

ACS Appl. Mater. Interfaces

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“…PCR was conducted using the GeneAmp ® 9700 PCR thermocycler (ABI, Foster City, CA, United States). Sequencing data were processed and analyzed as described in our previous study [1,2,33,34] and deposited in the NCBI Sequence Read Archive (SRA) database (Accession Number: PRJNA998735).…”

Section: Experimental Site Plant Materials and Sample Collectionmentioning

confidence: 99%

“…Alpha-diversity, non-metric multidimensional scaling analysis (NMDS), principal co-ordinates analysis (PCoA) (Bray-Curtis distance), partial least squares discriminant analysis (PLS-DA), microbial community composition, Venn diagram analysis, linear discriminant analysis (LDA), and LDA effect size (LEfSe) analysis of microbial communities were conducted as described in our previous studies [1,2,[32][33][34]. Networkx was used to calculate the correlation among the top-50 abundant microbial genera and construct the correlation network based on the Spearman coefficient (r ≥ 0.5, P < 0.05).…”

Section: Statistical Analysesmentioning

confidence: 99%

Analysis of metabolome and microbiome revealed the resistance mechanisms in sugarcane cultivars with high resistance to pokkah boeng disease

Xiao,

Chen,

Liang

et al. 2024

Chem. Biol. Technol. Agric.

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Background Endophytes are reported to play a role in resistance against plant pathogens. Understanding the metabolite-mediated endophytic microbiota composition in plants provides insights to improve plant stress resistance. In this study, via metabolome and microbiome analyses, we aimed to elucidate the resistance mechanism of sugarcane cultivars with high resistance to sugarcane pokkah boeng disease (PBD). The endophytic microbial composition and metabolites in the stems of various sugarcane cultivars with high resistance (HR) or high susceptibility (HS) to PBD were analyzed. Results The results revealed that the endophytic fungi with biocontrol effects such as Shinella, Dechloromonas, and Microbacter were significantly enriched, and the abundance of pathogenic fungi such as Fusarium, Ramichloridium, Scleroramularia, Phaeosphaeriopsis, Sarocladium, Zygophiala, Gibberella, Pseudocercospora, Cyphellophora, Monocillium, Apiotrichum, Microsphaeropsis, and Scleroramularia significantly reduced in the stems of HR cultivars. Additionally, six metabolites [citric acid, isocitrate, malic acid, PC(16:0/0:0), phosphocholine, and lysoPC(16:0)] were significantly related to the endophytes in the stems of HR cultivars. Conclusions These results suggested that more abundance of antagonistic microbes and highly active metabolic functions of endophytes in the HR cultivars were the important mechanisms underlying their higher resistance to PBD. Graphical abstract

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“…The extraction, PCR amplification, and sequencing of the total genomic DNA of the soil samples were completed according to previous protocols [36]. The primer pair 515F (5 -GTGCCAGCMGCCGCGGTAA-3 ) and 806R (5 -GGACTACHVGGGTWTCTAAT-3 ) was used to amplify the soil bacterial 16S rRNA gene in the V4 hypervariable region [38,39]. A library was constructed by using the TruSeq ® DNA PCR-Free Sample Preparation Kit (Illumina, San Diego, CA, USA), and Qubit and Q-PCR were used to quantify the constructed library.…”

Section: Analysis Of Soil Chemical Properties and Soil Bacterial Dive...mentioning

confidence: 99%

“…sph.harvard.edu/galaxy/root?tool_id=lefse_upload, accessed on 2 April 2023) was used to perform linear discriminant analysis (LDA) on samples according to different grouping conditions based on the taxonomic composition to identify clusters that had a significant differential (p < 0.05, LDA ≥ 3.0) impact on sample delineation. FAPROTAX [38] was employed to predict the bacterial functions involved in processes of the biochemical cycle as an artificially constructed functional classification database. The "pheatmap" package was run with the R (Version 3.3.1) software to conduct a heatmap analysis of the Spearman correlation between the soil chemical properties and soil bacteria.…”

Section: Analysis Of Soil Chemical Properties and Soil Bacterial Dive...mentioning

confidence: 99%

Long-Term Chemical Fertilization Drove Beneficial Bacteria for Rice Soil to Move from Bulk Soil to the Rhizosphere

et al. 2023

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Overuse of chemical fertilizer (CF) causes damage to soil and the environment. To reveal the process of the response of crop rhizospheric and bulk soil fertility and the bacterial community to long-term CF conditions, CF application and nonfertilization (CK, control) treatments were used in a long-term (12-year) fertilization experiment. Long-term CF application significantly increased the soil organic matter, total nitrogen, and available phosphorus contents (p < 0.05), increased the available nitrogen (AN) and potassium (AK) contents to varying degrees, and decreased the soil pH in both rice rhizospheric soil and bulk soil. In addition, the bacterial Shannon and Ace indices in rice rhizospheric soil under the CF treatment were all higher than those under the control (CK) treatment, and the bulk soil bacteria showed the opposite trend. The LEfSe results showed that unidentified_Gammaproteobacteria and Geobacter (genera) were significantly enriched in the rhizospheric and bulk soil of rice under the CK treatment, respectively. Gemmatimonadetes (phylum) and Nitrospirae (phylum) + Thiobacillus (genus) were significantly enriched in the rice rhizospheric and bulk soil under the CF treatment. Only AK and AN had strong positive correlations with soil bacteria. Long-term CF application accelerated the migration of soil bacteria from the bulk soil to the rhizosphere, thus improving soil fertility and nutrient cycling.

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Response of endophytic bacteria in sugarcane roots to different slow-release fertilizers with dicyandiamide (DCD) and humic acid (HA) applications

Cited by 5 publications

References 49 publications

Humic Acid-Functionalized Lignin-Based Coatings Regulate Nutrient Release and Promote Wheat Productivity and Grain Quality

Humic Acid-Functionalized Lignin-Based Coatings Regulate Nutrient Release and Promote Wheat Productivity and Grain Quality

Analysis of metabolome and microbiome revealed the resistance mechanisms in sugarcane cultivars with high resistance to pokkah boeng disease

Long-Term Chemical Fertilization Drove Beneficial Bacteria for Rice Soil to Move from Bulk Soil to the Rhizosphere

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