Response of Diaphorina citri (Hemiptera: Liviidae) to volatiles characteristic of preferred citrus hosts

Amorós, María Eugenia; Neves, Virginia Pereira das; Rivas, Fernando; Buenahora, José; Martini, Xavier; Stelinski, Lukasz L.; Rossini, Cármen

doi:10.1007/s11829-018-9651-8

Cited by 15 publications

(7 citation statements)

References 34 publications

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“…The chemical ecology of D. citri and its interactions with its host plants have been studied and the results showed that chemical volatiles from the Rutaceae are important for D. citri to locate host plants. [20][21][22][23] Among the many host plant volatiles, linalool, (E)-⊎-ocimene, citronellal, limonene, citral and ⊎-caryophyllene are major chemicals in new flush shoots or fresh leaves. 21,24 Additionally, CLas-infected citrus plants releasing higher levels of methyl salicylate have been found to be more attractive to D. citri than non-infected citrus plants.…”

Section: Introductionmentioning

confidence: 99%

An odorant‐binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles

Liu

Jiang

Fan

et al. 2021

Pest Management Science

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BACKGROUND: Odorant-binding proteins (OBPs) in insects contribute to the sensitivity of the olfactory system and connect external odorants to olfactory receptor neurons. Determination of the chemosensory functions in Diaphorina citri, a vector of the citrus Huanglongbing pathogen, may help in developing a potential target for pest management. RESULTS: Diaphorina citri showed dose-dependent electroantennogram recording (EAG) responses to 12 host plant volatiles. A two-choice behavioral trap experiment showed that four compounds (methyl salicylate, linalool, citral and R-(+)-limonene) that elicited high EAG responses also had significant attraction to adults. The expression profiles induced by these compounds were detected in nine OBP genes, DcitOBP1-9. DcitOBP3, DcitOBP6 and DcitOBP7 commonly showed significant upregulation or downregulation compared with the control. Microscale thermophoresis (MST) showed that the recombinant protein DcitOBP7 had high in vitro binding affinities (K d < 10 ∼M) to methyl salicylate, linalool and R-(+)-limonene, and moderate binding affinity to citral with a K d value of 15.95 ∼M. Furthermore, RNA interference (RNAi)-suppressed messenger RNA (mRNA) expression of DcitOBP7 resulted in a significant reduction in EAG activity and in adult D. citri behavioral responses to tested volatiles and the preferred host, Murraya paniculata. The hydrophilic residue Arg107 of DcitOBP7 may have a key role in binding odorants via formation of hydrogen bonds.CONCLUSION: These results show that DcitOBP7 plays an important role in the olfactory response. This finding may provide new insight into the functions of OBP families in D. citri and aid in the development of safe strategies for managing D. citri populations.

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Section: Introductionmentioning

confidence: 99%

An odorant‐binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles

Liu

Jiang

Fan

et al. 2021

Pest Management Science

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“…Other species such as the carrot psyllid Trioza apicalis Foerster, 1848 exhibit a strong response to hexane extracts of Daucus carota L. (Kristoffersen et al 2008). On the contrary, another natural vector of HLB, D. citri did not exhibit any behavioural response to individual volatiles emitted by Duncan grapefruit (Citrus paradisi Macfayden) and sweet orange (Citrus sinensis L.) (Amorós et al 2019). Accordingly, these authors suggested that a complex blend would be necessary to induce a higher number of catches on yellow sticky traps in a context of pest control.…”

Section: Discussionmentioning

confidence: 93%

Host plant preference of Trioza erytreae on lemon and bitter orange plants

Benhadi‐Marín

Garzo

Moreno

et al. 2021

Arthropod-Plant Interactions

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Trioza erytreae, the African citrus psyllid, is a vector of Candidatus Liberibacter spp., the causal agent of the citrus greening disease or Huanglongbing (HLB). The spread of the vector throughout the Iberian Peninsula has been continuous since its introduction in mainland Spain in 2014. The patterns of host preference and feeding behaviour largely depend on olfactory cues. Understanding these patterns is crucial to prevent further dispersion and develop management measures against the pest. In this work, a series of settlement, olfactometric, probing, and feeding experiments were conducted to assess the host preference of T. erytreae for lemon or bitter orange plants. The settlement experiment provided evidence on the preference of both sexes of T. erytreae for lemon plants, whereas males did not show any significant choice pattern in the case of the olfactometric assays. Forty EPG variables were analysed to describe and compare the probing and feeding behaviour of T. erytreae on lemon and bitter orange plants. The EPG variables indicated that T. erytreae has some difficulties in accepting the phloem of bitter orange plants. This suggests that lemon plants would be a better source for the acquisition of Candidatus Liberibacter asiaticus (CLas) by T. erytreae since the psyllid spends much longer periods feeding from the phloem on lemon than on bitter orange.

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“…Formic acid, acetic acid, and p-cymene are phagostimulatory to D. citri ( Lapointe et al 2016 ) and these volatiles have been explored for incorporation into a multi-modal AK device for this pest ( George and Lapointe 2017 ). While often composed mainly of terpenes characteristic of citrus leaves, the various kairomone blends demonstrated to attract D. citri thus far have varied in their chemical composition and only cause a 2- to 2.5-fold catch increase on yellow traps as compared with unbaited controls ( Patt and Setamou 2010 , Patt et al 2011 , Coutinho-Abreu et al 2014 , Amorós et al 2019 ). Collectively, these outcomes suggest that D. citri may use several general volatiles associated with citrus during host finding rather than a specific olfactory cue, also relying on other sensory modalities for host finding (e.g., vision; Paris et al 2017 , Khadka et al 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…Over the past decade, various blends of volatile organic compounds (VOCs) have been shown to exhibit some attractiveness to D. citri ; however, their effects have not been considered potent and reliable. Patt and Setamou (2010) identified an attractant blend for D. citri based on the VOCs released by leaf flush; other blends were identified based on VOCs from citrus varieties preferred by D. citri ( Amorós et al 2019 ). Gas chromatography coupled with electroantennography studies identified VOCs that also increased attraction of D. citri to yellow traps ( Coutinho-Abreu et al 2014 ).…”

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confidence: 99%

Progress Toward an Attract-and-Kill Device for Asian Citrus Psyllid (Hemiptera: Liviidae) Using Volatile Signatures of Citrus Infected With Huanglongbing as the Attractant

Martini

Hoyte

Mafra‐Neto

et al. 2020

Journal of Insect Science

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Asian citrus psyllid, Diaphorina citri (Kuwayama), preferentially orient toward citrus hosts infected with the phytopathogenic bacterium, Candidatus liberibacter asiaticus (CLas) the agent of citrus greening (Huanglongbing, HLB), compared to uninfected counterparts. We investigated whether this preference for the odors of infected plants could be useful for the development of an attract-and-kill (AK) device for D. citri. Twenty-nine blends of volatile organic compounds derived from the odor of citrus infected with CLas were tested in laboratory olfactometer tests, and two blends were also assessed under field conditions. A seven component blend of tricosane: geranial: methyl salicylate: geranyl acetone: linalool: phenylacetaldehyde: (E)-β-ocimene in a 0.40: 0.06: 0.08: 0.29: 0.08: 0.06: 0.03 ratio released from a proprietary slow-release matrix attracted twice more D. citri to yellow sticky traps compared with blank control traps. The attractive blend was subsequently co-formulated with spinosad insecticide into a slow-release matrix to create a prototype AK formulation against D. citri. This formulation effectively reduced the population density of D. citri up to 84% as measured with tap counts when deployed at a density of eight 2.5 g dollops per tree as compared with untreated controls in small plot field trials conducted in citrus orchards. Psyllid populations were not statistically affected at a deployment rate of four dollops per tree. Our results indicate that an AK formulation incorporating spinosad and a volatile blend signature of citrus greening into a slow-release matrix may be useful to suppress D. citri populations.

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Response of Diaphorina citri (Hemiptera: Liviidae) to volatiles characteristic of preferred citrus hosts

Cited by 15 publications

References 34 publications

An odorant‐binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles

An odorant‐binding protein of Asian citrus psyllid, Diaphorina citri, participates in the response of host plant volatiles

Host plant preference of Trioza erytreae on lemon and bitter orange plants

Progress Toward an Attract-and-Kill Device for Asian Citrus Psyllid (Hemiptera: Liviidae) Using Volatile Signatures of Citrus Infected With Huanglongbing as the Attractant

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