Response of denitrifying community, denitrification genes and antibiotic resistance genes to oxytetracycline stress in polycaprolactone supported solid-phase denitrification reactor

Pathogenic and Indigenous Denitrifying Bacteria are Transcriptionally Active and Key Multi-Antibiotic-Resistant Players in Wastewater Treatment Plants

“…Besides, 306 out of 496 ARGs were in the MAGs of potential denitrifiers, revealing that denitrifying bacteria are important hosts of diverse ARGs in WWTPs (Dataset S2). The high prevalence of ARGs in denitrifiers was reasonable because there was some evidence showing that the presence of antibiotics would cause a significant inhibition to denitrification genes. − Considering the presence of various antibiotics in WWTPs (Dataset S8), denitrifiers carrying ARGs could better maintain their denitrifying function and protect themselves from inhibition by the antibiotics. When both taxonomic affiliation and nitrogen removal function of the 248 MAGs were considered, we found that multi-antibiotic-resistant Proteobacteria (58/88 MAGs, 65.9%) played a predominant role in the nitrification and denitrification, while Patescibacteria (66/68 MAGs, 97.1%) and Bacteroidota (26/39 MAGs, 66.7%) were dominated by nonresistant or single-resistant populations without a detectable NDG (Dataset S2).…”

Insight into the characteristics of microbial communities in a single-stage anammox reactor under different oxygen conditions

“…In addition, the genera Caldithrix , Ignavibacterium , Limnobacter , Phycisphaera , Solitalea , and Sphaerobacter were reported to be capable of reducing nitrite or nitrate, which also showed the highest relative abundance under the micro-aerobic condition. 19–23 Fig. 4B shows that Caldilineaceae and Anaerolineaceae that belong to the Chloroflexi phylum were the dominant heterotrophic families among the three systems.…”

Biodegradation of antibiotics by denitrification and their effects on the physiology, kinetics, and denitrifying microbial communities

“…Diferentes autores han observado que una posible causa por la que disminuye la abundancia de los genes desnitrificantes es por el efecto negativo de los antibióticos sobre los hospederos que los contienen (Wu et al, 2017;Feng et al, 2020). La disminución tanto del número de copias como de la abundancia relativa de los genes nirS y nosZ se ha observado ante la exposición de diferentes tetraciclinas (TET u OTC), sulfonamidas (SMT) o bien mezclas de estos antibióticos, disminuyendo entre el 17-48% y 50-65% respectivamente.…”