Response of crested wheatgrass (Agropyron cristatum L.), perennial ryegrass (Lolium perenne and white clover (Trifolium repens L.) to inoculation with Bacillus polymyxa

Holl, F. B.; Chanway, C. P.; Turkington, Roy; Radley, R. A.

doi:10.1016/0038-0717(88)90121-6

Cited by 135 publications

(54 citation statements)

References 24 publications

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“…Inoculation by B. polymyxa CF43 has been shown to stimulate the yield of spring wheat (6). This and other observations (16,17) have confirmed the plant growth-promoting rhizobacterial character of B. polymyxa.…”

supporting

confidence: 83%

Cloning, sequencing, and disruption of a levanase gene of Bacillus polymyxa CF43

1994

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The Bacillus polymyxa CF43 leLk gene, expressing both sucrose and fructan hydrolase activities, was isolated from a genomic library of B. polymyxa screened in Bacillus subtilis. The gene was detected as expressing sucrose hydrolase activity; B. subtilis transformants did not secrete the lelA gene product (LelA) into the extracellular medium. A 1.7-kb DNA fragment sufficient for le4 expression in Escherichia coli was sequenced. It contains a 548-codon open reading frame. The deduced amino acid sequence shows 54% identity with mature B. subtilis levanase and is similar to other fructanases and sucrases (Pi-D-fructosyltransferases). Multiple-sequence alignment of 14 of these proteins revealed several previously unreported features. LeIA appears to be a 512-amino-acid polypeptide containing no canonical signal peptide. The hydrolytic activities of LelA on sucrose, levan, and inulin were compared with those of B. subtilis levanase and sucrase, confirming that LelA is indeed a fructanase. The lek gene in the chromosome of B. polymyxa was disrupted with a chloramphenicol resistance gene (cat) by "inter-gramic" conjugation: the lelA::cat insertion on a mobilizable plasmid was transferred from an E. coli transformant to B. polymyxa CF43, and B. polymyxa transconjugants containing the leL4::cat construct replacing the wild-type leLA gene in their chromosomes were selected directly. The growth of the mutant strain on levan, inulin, and sucrose was not affected.Exopolysaccharides appear to play a role in the early steps of plant colonization by symbiotic or phytopathogenic bacteria such as Rhizobium spp. or Agrobacterium spp., respectively (23). Free-living rhizobacteria belonging to the genus Bacillus have been isolated from the rhizosphere of Graminae spp., including wheat and maize (7,24,26,32

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supporting

confidence: 83%

Cloning, sequencing, and disruption of a levanase gene of Bacillus polymyxa CF43

1994

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“…The effi cacy of Bacillus sp. to enhance nodulation, plant dry matter and grain yield on coinoculation with rhizobia has also been reported for other legumes like white clover 5 , pigeon pea 7 and peanut 31 . PGPR isolated from the maize rhizosphere including Bacillus, Pseudomonas and Serratia were reported to improve the yield by 9-14% 32 .…”

Section: Effect Of Coinoculation Of Bacillus Strains With Mesorhizobimentioning

confidence: 60%

“…Usually, these rhizosphere bacteria have been identifi ed to cause stimulation of plant growth, protection of plants from pathogens, or cause degradation of xenobiotics in the rhizosphere. For example, application of Bacillus species to seeds or roots has been shown to cause alteration in the composition of rhizosphere leading to increase in growth and yield of different crops 2,[5][6][7] . Disease suppression of alfalfa by B. cereus 8 , take-all of wheat by B. polymyxa 9,10 , reduction in pigeon pea wilt by B. subtilis strain 11 has been reported.…”

Section: Introductionmentioning

confidence: 99%

Improvement in symbiotic efficiency of chickpea (Cicer arietinum) by coinoculation of Bacillus strains with Mesorhizobium sp. Cicer

2007

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Rhizobacteria belonging to Bacillus sp. were isolated from the rhizosphere of chickpea (Cicer arietinum). Ten Bacillus strains were studied for their antifungal activity, effect on seedling emergence and plant growth promotion. Two Bacillus strains CBS127 and CBS155 inhibited the growth of all the four pathogenic fungi tested on nutrient agar medium plates in vitro. Seed inoculation with different Bacillus strains showed stimulatory effect on root and shoot growth at 10 d of observation in comparison to control whereas four Bacillus strains CBS24, CBS127, CBS129 and CBS155 caused retardation of shoot growth at 10 d. Maximum nodule-promoting effect was observed with Bacillus strains CBS106, CBS127 and CBS155. The symbiotic effectiveness of Mesorhizobium sp. Cicer strain Ca181 was further improved on coinoculation with six Bacillus strains i.e. CBS9, CBS17, CBS20, CBS106, CBS127 and CBS155 at 80 d of plant growth under sterile conditions and shoot dry weight ratios increased 1.62 to 1.74 times those of Mesorhizobium-inoculated treatments, suggesting the usefulness of introduced rhizobacteria in improving crop productivity.

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“…The PGPR strains belonging to Paenibacillus polymyxa (1) promote plant growth by producing indole-3-acetic acid (IAA) (9) and volatile compounds (14). They are also known to suppress fungal phytopathogens (2, 5-7, 13, 31, 34) and plant-parasitic nematodes (12,29).…”

mentioning

confidence: 99%

The Genome of the Plant Growth-Promoting Rhizobacterium Paenibacillus polymyxa M-1 Contains Nine Sites Dedicated to Nonribosomal Synthesis of Lipopeptides and Polyketides

Niu

Rueckert

Blom

et al. 2011

Journal of Bacteriology

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The genome of Paenibacillus polymyxa M-1 consisted of a 5.8-Mb chromosome and a 360-kb plasmid. Nine sites were dedicated to nonribosomal synthesis of lipopeptides and polyketides. Eight of them were located at the chromosome, while one gene cluster predicted to encode an unknown secondary metabolite was present on the plasmid.Plant growth-promoting rhizobacteria (PGPR) have been applied as environmentally friendly alternatives to agrochemicals to improve crop yield and quality (16). The PGPR strains belonging to Paenibacillus polymyxa (1) promote plant growth by producing indole-3-acetic acid (IAA) (9) and volatile compounds (14). They are also known to suppress fungal phytopathogens (2, 5-7, 13, 31, 34) and plant-parasitic nematodes (12,29). Due to its action as a biocontrol agent, P. polymyxa produces several peptide antibiotics (3, 8, 10, 11, 15, 18, 20, 22-24, 26-28, 32) which might be important in control of plant pathogens (25).Strain M-1, isolated from surface-sterilized wheat root tissues, was identified by 16S rRNA gene sequencing and by physiological and biochemical analysis as being P. polymyxa (33). It is capable of colonizing root surfaces of wheat, promoting wheat growth, and suppressing wheat sharp eyespot disease. In addition, it acts antagonistically against several phytopathogens in vitro by producing antibiotics, including fusaricidin and polymyxin, and by secreting hydrolytic enzymes, for example, endo-␤-1,3-glucanase.Genomic DNA prepared from M-1 was used for construction of a 3-kb-long paired-end library with a GS FLX library preparation kit in combination with GS FLX paired-end adaptors (both from Roche, Mannheim, Germany) according to the manufacturer's protocol. The reads were assembled using the GS De Novo Assembler software program, and the resulting scaffolds were oriented based on the occurrence of unique single nucleotide polymorphisms (SNPs) in the repetitive rRNA (RRN) contigs. In total, 869,907 reads, including 312,451 paired reads, were assembled with a total of 185,008,620 bp. Utilization of the paired-end information allowed scaffolding of the 55 contigs larger than 500 bp into 16 scaffolds containing 45 contigs. Gap closure was done by long-range PCR (using Phusion polymerase; New England BioLabs, Frankfurt [Main], Germany) and subsequent Sanger sequencing (IIT Biotech, Bielefeld, Germany). Prediction of protein-encoding sequences was initially accomplished with the REGANOR server (17). Manual and automatic annotation was done using the annotation software program GenDB 2.4 (19).The complete genome sequence of M-1 consisted of a circular 5,864,546-bp chromosome and a 366,576-bp plasmid, with GϩC values of 54.58% and 37.61%, respectively. Five thousand sixty-one genes (CDS), 14 rRNA operons, and 110 tRNAs resided in the chromosome, while 345 genes were located on the plasmid. Many important genes were found to be plasmid linked, such as those encoding ribosomal proteins and genes involved in replication, repair and methylation, transcription, translation initiation, metabol...

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Response of crested wheatgrass (Agropyron cristatum L.), perennial ryegrass (Lolium perenne and white clover (Trifolium repens L.) to inoculation with Bacillus polymyxa

Cited by 135 publications

References 24 publications

Cloning, sequencing, and disruption of a levanase gene of Bacillus polymyxa CF43

Cloning, sequencing, and disruption of a levanase gene of Bacillus polymyxa CF43

Improvement in symbiotic efficiency of chickpea (Cicer arietinum) by coinoculation of Bacillus strains with Mesorhizobium sp. Cicer

The Genome of the Plant Growth-Promoting Rhizobacterium Paenibacillus polymyxa M-1 Contains Nine Sites Dedicated to Nonribosomal Synthesis of Lipopeptides and Polyketides

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