Response of antioxidant system to drought stress and re-watering in Alfalfa during branching

Tina, R R; Shan, Xiaofei; Wang, Y; Guo, Shuang; Mao, Bing; Wang, W; Wu, Haoping; Zhao, Tao

doi:10.1088/1755-1315/94/1/012129

Cited by 7 publications

(8 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Regarding ROS enzymatic defence, accumulation of SOD appears to be one of the first lines of defence, converting superoxide radicals to H 2 O 2 . The enzymes POD and CAT can then catalyse the resulting H 2 O 2 into harmless water and oxygen (Cao et al ., 2017; Tina et al, 2017). Our results revealed that the level of SOD and POD increased under higher Cu concentrations, which indicates that these enzymes provide a good defence mechanism against Cu stress.…”

Section: Discussionmentioning

confidence: 99%

Analysis of physiological and metabolite response of Celosia argentea to copper stress

Wang

et al. 2021

Plant Biol J

View full text Add to dashboard Cite

Copper‐tolerant (Cu) plants with high ornamental value play an important role in the ecological restoration of the copper tail mining area. We first discovered Celosia argentea adaptability in a copper mine area in China; however, its resistance to Cu and the underlying mechanism are not clear. In this study, C. argentea was selected for pot culture experiments. Its heavy metal accumulation and translocation, physiological and metabolic products were analysed under different growth concentrations of Cu (0–2400 mg.kg−1) stress. Our results indicated that roots strongly accumulated Cu2+. Oxidative stress defence mechanisms were activated in leaves under Cu treatment. Higher Cu concentrations triggered higher electrolyte leakage (EL), Malondialdehyde (MDA), superoxide dismutase (SOD) and peroxidase (POD) activity, and consequently a higher capacity to scavenge oxygen radicals and maintain cellular membrane integrity. In the citrate cycle, some amino acids and sugars related to biological pathways were altered in C. argentea exposed to Cu stress. Metabolomics data revealed that C. argentea used elevated sugar content as an antioxidant to regulate reactive oxygen species (ROS). Some organic acids and amino acids were up‐regulated compared with the control, indicating that these may chelate Cu in cells to remove excess Cu2+. The up‐regulation of polyamines and some organic acids may mitigate oxidative stress. These results indicate that C. argentea could be used as a Cu‐tolerant plant in Cu mine restoration. Its Cu tolerance mechanism also provides a basis for future plant improvement or breeding for use in mine restoration.

show abstract

Section: Discussionmentioning

confidence: 99%

Analysis of physiological and metabolite response of Celosia argentea to copper stress

Wang

et al. 2021

Plant Biol J

View full text Add to dashboard Cite

show abstract

“…Moreover, drought can lead to the accumulation of active oxygen substances in the leaves, which may accelerate the peroxidation of biological membrane lipid to produce toxic products, thereby inhibiting plant growth [ 5 ]. For plants exposed to drought stress, the total primary productivity is not only closely related to their resistance and tolerance to drought stress, but also shows an important relationship with the ability of plants to recover from damage after the elimination of the stress [ 6 , 7 ]. Therefore, the recovery ability after rehydration is important for the successful adaptation of plants to arid environments.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the recovery ability after rehydration is important for the successful adaptation of plants to arid environments. Rehydration helps plants recover their physiological functions, and it can offset plant damage from drought stress to a certain extent [ 7 ]. However, the compensation of rehydration to plant growth after drought stress is often limited.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome profiling reveals the effects of drought tolerance in Giant Juncao

et al. 2021

View full text Add to dashboard Cite

Background Giant Juncao is often used as feed for livestock because of its huge biomass. However, drought stress reduces forage production by affecting the normal growth and development of plants. Therefore, investigating the molecular mechanisms of drought tolerance will provide important information for the improvement of drought tolerance in this grass. Results A total of 144.96 Gb of clean data was generated and assembled into 144,806 transcripts and 93,907 unigenes. After 7 and 14 days of drought stress, a total of 16,726 and 46,492 differentially expressed genes (DEGs) were observed, respectively. Compared with normal irrigation, 16,247, 23,503, and 11,598 DEGs were observed in 1, 5, and 9 days following rehydration, respectively. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses revealed abiotic stress-responsive genes and pathways related to catalytic activity, methyltransferase activity, transferase activity, and superoxide metabolic process. We also identified transcription factors belonging to several families, including basic helix-loop-helix (bHLH), WRKY, NAM (no apical meristem), ATAF1/2 and CUC2 (cup-shaped cotyledon) (NAC), fatty acyl-CoA reductase (FAR1), B3, myeloblastosis (MYB)-related, and basic leucine zipper (bZIP) families, which are important drought-rehydration-responsive proteins. Weighted gene co-expression network analysis was also used to analyze the RNA-seq data to predict the interrelationship between genes. Twenty modules were obtained, and four of these modules may be involved in photosynthesis and plant hormone signal transduction that respond to drought and rehydration conditions. Conclusions Our research is the first to provide a more comprehensive understanding of DEGs involved in drought stress at the transcriptome level in Giant Juncao with different drought and recovery conditions. These results may reveal insights into the molecular mechanisms of drought tolerance in Giant Juncao and provide diverse genetic resources involved in drought tolerance research.

show abstract

“…Furthermore, overexpression of AtHB13 in Arabidopsis and HaHB1 in sunflower can significantly improve the cold resistance of transgenic plants (Cabello, Arce & Chan, 2012); the maize HD-Zip gene Zmhdz10 enhances the drought resistance of transgenic rice and Arabidopsis (Zhao et al, 2014). In contrast, MtHB1 reduces the drought resistance of alfalfa by inhibiting lateral root formation (Tina et al, 2017). Moreover, ATHB52 can respond to light and regulate photomorphogenesis and de-etiolation (Henriksson, 2005), and AtHB1 responds to short-day photoperiods and promotes hypocotyl and root elongation (Capella et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Genome-wide identification, characterization and expression analysis of the HD-Zip gene family in the stem development of the woody plant Prunus mume

Zheng

Zhuo

et al. 2019

PeerJ

View full text Add to dashboard Cite

The homeodomain-leucine zipper (HD-Zip) gene family, a group of plant-specific transcriptional factors (TFs), participates in regulating growth, development, and environmental responses. However, the characteristics and biological functions of HD-Zip genes in Prunus mume, which blooms in late winter or early spring, have not been reported. In this study, 32 HD-Zip genes, named PmHB1–PmHB32 based on their chromosomal positions, were identified in the genome of P. mume. These genes are distributed among seven chromosomes and are phylogenetically clustered into four major groups. Gene structure and motif composition were mostly conserved in each group. The Ka/Ks ratios showed that purifying selection has played a leading role in the long-term evolution of the genes, which maintained the function of this family. MicroRNA target site prediction indicated that the genes of the HD-Zip III subfamily may be regulated by miR165/166. Expression pattern analysis showed that the 32 genes were differentially expressed across five different tissues (leaf, flower bud, stem, fruit, and root) and at different stages of stem and leaf-bud development, suggesting that 10 of the genes may play important roles in stem development. Protein–protein interaction predictions showed that the subfamily III genes may regulate vascular development and shoot apical meristem (SAM) maintenance. Promoter analysis showed that the HD-Zip III genes might be involved in responses to light, hormones, and abiotic stressors and stem development. Taken together, our results provide an overview of the HD-Zip family in P. mume and lay the foundation for the molecular breeding of woody ornamental plants.

show abstract

Response of antioxidant system to drought stress and re-watering in Alfalfa during branching

Cited by 7 publications

References 3 publications

Analysis of physiological and metabolite response of Celosia argentea to copper stress

Analysis of physiological and metabolite response of Celosia argentea to copper stress

Transcriptome profiling reveals the effects of drought tolerance in Giant Juncao

Genome-wide identification, characterization and expression analysis of the HD-Zip gene family in the stem development of the woody plant Prunus mume

Contact Info

Product

Resources

About