Response of an Antarctic Lake Heterotrophic Community to High Dissolved Oxygen

Mikell, A. T.; Parker, Barry; Simmons, George M.

doi:10.1128/aem.47.5.1062-1066.1984

Cited by 21 publications

(3 citation statements)

References 24 publications

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“…lists the physiological and morphological characteristics of the six VHO-selected Lake Hoare isolates. Although <35% of planktonic ADO-selected CFU survived HDO conditions, <1% of ADO-selected CFU formed colonies with VHO incubation(25). All of the bacterial isolates in this study appeared similar on the basis of morphology, motility, gram-negative reaction, possession of oxidase, and pigmentation.…”

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confidence: 61%

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Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake

Mikell

Parker

Gregory

1986

Appl Environ Microbiol

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We sought to determine factors relating to the survival of heterotrophic microorganisms from the high-dissolved-oxygen (HDO) waters of Lake Hoare, Antarctica. This lake contains perpetual HDO about three times that of normal saturation (40 to 50 mg liter −1 ). Five isolates, one yeast and four bacteria, were selected from Lake Hoare waters by growth with the membrane filter technique with oxygen added to yield dissolved concentrations 14 times that in situ, 175 mg liter −1 . One bacterial isolate was obtained from the microbial mat beneath the HDO waters. This organism was isolated at normal atmospheric oxygen saturation. The bacteria were gram-negative rods, motile, oxidase positive, catalase positive, and superoxide dismutase positive; they contained carotenoids. The planktonic isolates grew in media containing 10 mg of Trypticase soy (BBL Microbiology Systems)-peptone (2:1) liter −1 but not at 10 g liter −1 . Under low-nutrient levels simulating Lake Hoare waters (10 mg liter −1 ), two of the planktonic isolates tested were not inhibited by HDO. Growth inhibition by HDO increased as nutrient concentration was increased. A carotenoid-negative mutant of one isolate demonstrated a decreased growth rate, maximal cell density, and increased cell lysis in the death phase under HDO compared with the parent strain. The specific activity of superoxide dismutase was increased by HDO in four of the five bacterial isolates. The superoxide dismutase was of the manganese type on the basis of inhibition and electrophoretic studies. The bacterial isolates from Lake Hoare possess several adaptations which may aid their survival in the HDO waters, as well as protection due to the oligotrophic nature of the lake.

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confidence: 61%

“…lation-respiration by HDO in plankton samples from Lake Hoare, Antarctica. Only CFU formation was inhibited by HDO in Lake Hoare samples relative to ADO (25). Lake Hoare is oligotrophic, and its perennial ice cover isolates the water from the atmosphere, allowing the development of HDO (27).…”

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confidence: 95%

Factors Affecting High-Oxygen Survival of Heterotrophic Microorganisms from an Antarctic Lake

Mikell

Parker

Gregory

1986

Appl Environ Microbiol

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“…The microbial mats found today in these environments are composed primarily of cyanobacteria, heterotrophic bacteria, protozoan cysts, eukaryotic algal cells and minerals associated with microbial activity occurring throughout much of the benthic regions of the dry valley lakes (Wharton et al, 1983;Mikell et al, 1984;Vincent, 1988). In the Archean the photosynthesizers were distributed in the upper layers, while the anaerobic microorganisms, such as the sulfate-reducing Archaea were relegated to the lower layers.…”

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confidence: 99%