Response of a dog kidney cell line (MDCK) to antidiuretic hormone (ADH) with special reference to activation of protein kinase

Mita, Sachiko; Takeda, Kazuyoshi; Nakane, Motoyuki; Yasuda, Hideyo; Kawashima, Keisuke; Yamada, Mitsuhiro; Endo, Hiroyoshi

doi:10.1016/0014-4827(80)90053-1

Cited by 13 publications

(5 citation statements)

References 6 publications

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“…In our study no attempt was made to determine the presence of receptors for ADH; however, the response to this hormone in water fluxes is strongly suggestive of ADH receptors in the MDCK cells. Mita, Takeda, Nakene, Yasuda, Kawashima, Yamada & Endo (1980) reported activation of protein kinase in MDCK cells induced by vasopressin, which agrees with our suggestion of the presence of ADH receptors in these cells. Oien, Babiarz, Soderman, Ham & Kuehl (1979) reported the presence of receptors for PGE1 in rat kidney homogenates and they described two binding sites, one with a Ka of 2-2 x 107 M-1 and the other with a lower Ka (1-1 x 106 M-1).…”

Section: Discussionsupporting

confidence: 81%

Prostaglandin receptors and hormonal actions on water fluxes in cultured canine renal cells (MDCK line).

Martínez¹,

Reyes²

1984

The Journal of Physiology

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SUMMARY1. An established cellular line (MDCK) derived from canine kidney showed specific binding for prostaglandin E2, both when the determination of specific binding was carried out in cells attached to a semipermeable support and when this binding was determined in suspended cells in a calcium-free medium.2. Specific binding for tritiated prostaglandin F2z was also observed, and it was inhibited by increasing concentrations of non-radioactive PGF. in a dose-related pattern. Cross-reactivity between PGE2 binding and PGF2 was present.3. Arginine-vasopressin induced an increase in the apical-to-basal water flux when applied to the basolateral surface. No change was observed when the hormone was in contact with the apical surface of the monolayer.4. Prostaglandin E2 also induced an increase in water flux, with a slower time course than that of arginine-vasopressin. The presence of PGE2 inhibited the increase in water flux induced by antidiuretic hormone.5. The results suggest that MDCK monolayers depict a physiological antagonism between ADH and prostaglandins which is similar to that observed in natural epithelia.

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Section: Discussionsupporting

confidence: 81%

Prostaglandin receptors and hormonal actions on water fluxes in cultured canine renal cells (MDCK line).

Martínez¹,

Reyes²

1984

The Journal of Physiology

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Section: Discussionmentioning

confidence: 96%

“…These values are considerably higher than those recorded for isolated TAL segments(39) . MDCK cells produce elevated levels of cyclic AMP in the presence of vasopressin(36,40), and this observation has been used to support a distal tubule origin . However, this hormonal response is also common to the collecting duct in all species examined (2).It is not clear whether nephron segment-specific properties reflecting the origin of MDCK have been faithfully retained or modulated during growth in culture, or whether the various MDCK sublines in use are essentially identical or even homogeneous.…”

mentioning

confidence: 96%

The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule.

Herzlinger¹,

Easton²,

Ojakian³

1982

The Journal of Cell Biology

153

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Monoclonal antibodies were prepared against the Madin-Darby canine kidney (MDCK) cell line to identify epithelial cell surface macromolecules involved in renal function . Lymphocyte hybrids were generated by fusing P3U-1 myeloma cells with spleen cells from a C3H mouse immunized with MDCK cells. Hybridomas secreting anti-MDCK antibodies were obtained and clonal lines isolated in soft agarose. We are reporting on one hybridoma line that secretes a monoclonal antibody that binds to MDCK cells at levels 20-fold greater than background binding. Indirect immunofluorescence microscopy was utilized to study the distribution of antibody binding on MDCK cells and on frozen sections of dog kidney and several nonrenal tissues. In the kidney the fluorescence staining pattern demonstrates that the antibody recognizes an antigenic determinant that is expressed only on the epithelial cells of the thick ascending limb of Henle's loop and the distal convoluted tubule and appears to be localized on the basolateral plasma membrane . This antigen also has a unique distribution in non-renal tissues and can only be detected on cells known to be active in transepithelial ion movements . These results indicate the probable distal tubule origin of MDCK and suggest that the monoclonal antibody recognizes a cell surface antigen involved in physiological functions unique to the kidney distal tubule and transporting epithelia of nonrenal tissues.

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“…It has been shown that AVP increases intracellular cAMP generation in all three renal tubular cell lines used in this study [14, 15, 16], among which the MDCK cell line has been shown to be most responsive to AVP [17]. …”

Section: Discussionmentioning

confidence: 99%

Secretion of Adrenomedullin by Renal Tubular Cell Lines

Sato

Imai

Iwashina

et al. 1997

Nephron

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Adrenomedullin (AM) is a novel and potent vasodilator peptide originally isolated from human pheochromocytoma. The present study was designed to study whether AM is produced by and secreted from renal tubular cell lines and whether arginine vasopressin (AVP) affects AM secretion from these cell lines. Three renal tubular cell lines derived from different species (LLCPK1, MDCK, and MDBK) secrete AM-like immunoreactivity (AM-LI) into culture medium, the immunological and physicochemical properties of which are similar to that of synthetic human AM as evaluated by reverse-phase high-performance liquid chromatography. Among the three cell lines, AVP in combination with a phosphodiesterase inhibitor (isobutylmethylxanthine) stimulated AM-LI secretion most potently from MDCK cells in a time- and dose-dependent manner. In MDCK cells, a V₂ receptor agonist (deamino-D-Arg⁸-vasopressin) dose-dependently stimulated AM-LI secretion in the same manner as AVP. Furthermore, the AVP-induced AM-LI secretion was blocked by a V₂ receptor antagonist (OPC31260), but not by a V₁ receptor antagonist (OPC21268). These data indicate that AM is secreted from renal tubular cell lines and that AVP stimulates AM secretion via V₂ receptors, suggesting its autocrine/paracrine role in renal function.

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Response of a dog kidney cell line (MDCK) to antidiuretic hormone (ADH) with special reference to activation of protein kinase

Cited by 13 publications

References 6 publications

Prostaglandin receptors and hormonal actions on water fluxes in cultured canine renal cells (MDCK line).

Prostaglandin receptors and hormonal actions on water fluxes in cultured canine renal cells (MDCK line).

The MDCK epithelial cell line expresses a cell surface antigen of the kidney distal tubule.

Secretion of Adrenomedullin by Renal Tubular Cell Lines

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