Response mechanism of Vibrio parahaemolyticus at high pressure revealed by transcriptomic analysis

Liang, Shanquan; Zhang, Tan; Liu, Zhihao; Wang, Jingyu; Zhu, Chunshen; Kong, Qing; Fu, Xiaodan; Mou, Haijin

doi:10.1007/s00253-022-12082-y

Cited by 3 publications

(4 citation statements)

References 72 publications

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“…Next, we noticed one of the most abundant enrichments—CarM—which also plays a potential role in stress tolerance, including heat stress, polycyclic aromatic hydrocarbon degradation, and piezotolerance ( 43 , 55 , 56 ). Then, we confirmed that the numbers of upregulated DEGs in CarM were double than those of downregulated ones (Fig.…”

Section: Resultsmentioning

confidence: 98%

“…While yielding ATP is an energy-consuming process, we believed that to save more energy components to resist HHP, A. sydowii DM1 alternatively inhibited the respiratory chain and activated the TCA cycle for ATP production; as a result, more beneficial precursors or substances were obtained under elevated HHP with low energy consumption. However, a related study suggested that V. parahaemolyticus could use the energy transmitted by electrons to pump H + out of the cell under HHP stress for the purpose of reducing intracellular H + levels without consuming ATP ( 56 ). Given the potential impact of energy accumulation on cell growth and metabolism ( 82 , 83 ), it is imperative that future research is conducted to investigate ATP accumulation within cells under HHP, which will provide a clearer understanding of the ability of A. sydowii DM1 to utilize ATP in the synthesis of stress-resistant substances.…”

Section: Discussionmentioning

confidence: 99%

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Insight into the adaptation mechanisms of high hydrostatic pressure in physiology and metabolism of hadal fungi from the deepest ocean sediment

Zhong,

Li,

Deng

et al. 2024

mSystems

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High hydrostatic pressure (HHP) influences the life processes of organisms living at depth in the oceans. While filamentous fungi are one of the essential members of deep-sea microorganisms, few works have explored their piezotolerance to HHP. Here, we obtained three homogeneous Aspergillus sydowii from terrestrial, shallow, and hadal areas, respectively, to compare their pressure resistance. A set of all-around evaluation methods including determination of growth rate, metabolic activity, and microscopic staining observation was established and indicated that A. sydowii DM1 from the hadal area displayed significant piezotolerance. Global analysis of transcriptome data under elevated HHP revealed that A. sydowii DM1 proactively modulated cell membrane permeability, hyphae morphology, and septal quantities for seeking a better livelihood under mild pressure. Besides, differentially expressed genes were mainly enriched in the biosynthesis of amino acids, carbohydrate metabolism, cell process, etc., implying how the filamentous fungi respond to elevated pressure at the molecular level. We speculated that A. sydowii DM1 could acclimatize itself to HHP by adopting several strategies, including environmental response pathway HOG-MAPK, stress proteins, and cellular metabolisms. IMPORTANCE Fungi play an ecological and biological function in marine environments, while the physiology of filamentous fungi under high hydrostatic pressure (HHP) is an unknown territory due to current technologies. As filamentous fungi are found in various niches, Aspergillus sp. from deep-sea inspire us to the physiological trait of eukaryotes under HHP, which can be considered as a prospective research model. Here, the evaluation methods we constructed would be universal for most filamentous fungi to assess their pressure resistance, and we found that Aspergillus sydowii DM1 from the hadal area owned better piezotolerance and the active metabolisms under HHP indicated the existence of undiscovered metabolic strategies for hadal fungi. Since pressure-related research of marine fungi has been unexpectedly neglected, our study provided an enlightening strategy for them under HHP; we believed that understanding their adaptation and ecological function in original niches will be accelerated in the perceivable future.

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Section: Resultsmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Insight into the adaptation mechanisms of high hydrostatic pressure in physiology and metabolism of hadal fungi from the deepest ocean sediment

Zhong,

Li,

Deng

et al. 2024

mSystems

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“…Total RNA was extracted from the bacterial precipitation using TRIzol® Reagent Invitrogen (Life Technologies, Carlsbad, CA, USA) according to the manufacturer instructions and genomic DNA was removed using DNase I (Vazyme, Nanjing, China). The cDNA synthesis and gene amplification were performed following previously reported methods (Liang et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Response of Salmonella enterica serovar Typhimurium to alginate oligosaccharides fermented with fecal inoculum: integrated transcriptomic and metabolomic analyses

Cheng

Xiao

Ren

et al. 2023

Mar Life Sci Technol

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Alginate oligosaccharides (AOS), extracted from marine brown algae, are a common functional feed additive; however, it remains unclear whether they modulate the gut microbiota and microbial metabolites. The response of Salmonella enterica serovar Typhimurium, a common poultry pathogen, to AOS fermented with chicken fecal inocula was investigated using metabolomic and transcriptomic analyses. Single-strain cultivation tests showed that AOS did not directly inhibit the growth of S. Typhimurium. However, when AOS were fermented by chicken fecal microbiota, the supernatant of fermented AOS (F-AOS) exhibited remarkable antibacterial activity against S. Typhimurium, decreasing the abundance ratio of S. Typhimurium in the fecal microbiota from 18.94 to 2.94%. Transcriptomic analyses showed that the 855 differentially expressed genes induced by F-AOS were mainly enriched in porphyrin and chlorophyll metabolism, oxidative phosphorylation, and Salmonella infection-related pathways. RT-qPCR confirmed that F-AOS downregulated key genes involved in flagellar assembly and the type III secretory system of S. Typhimurium, indicating metabolites in F-AOS can influence the growth and metabolism of S. Typhimurium. Metabolomic analyses showed that 205 microbial metabolites were significantly altered in F-AOS. Among them, the increase in indolelactic acid and 3-indolepropionic acid levels were further confirmed using HPLC. This study provides a new perspective for the application of AOS as a feed additive against pathogenic intestinal bacteria.

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“…A variety of parameters are correlated with abundance and distribution in various regions and waterbody types and can be generally grouped into three categories: i) hydrological and/or meteorological factors, ii) abiotic factors that may be indicative of biological activity, and iii) purely biological factors. Additional considerations should be made when evaluating aquaculture studies, such as the now-known strains of Vp that are resistant to food pressure treatments [98,99]. An overview of environmental parameters potentially driving Vibrio success are provided here and in Table 2.…”

Section: Predictors Published Patterns and Correlated Ecological Factorsmentioning

confidence: 99%

Tools to Enumerate and Predict Distribution Patterns of Environmental Vibrio vulnificus and Vibrio parahaemolyticus

Waidner,

Potdukhe

2023

Microorganisms

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Vibrio vulnificus (Vv) and Vibrio parahaemolyticus (Vp) are water- and foodborne bacteria that can cause several distinct human diseases, collectively called vibriosis. The success of oyster aquaculture is negatively impacted by high Vibrio abundances. Myriad environmental factors affect the distribution of pathogenic Vibrio, including temperature, salinity, eutrophication, extreme weather events, and plankton loads, including harmful algal blooms. In this paper, we synthesize the current understanding of ecological drivers of Vv and Vp and provide a summary of various tools used to enumerate Vv and Vp in a variety of environments and environmental samples. We also highlight the limitations and benefits of each of the measurement tools and propose example alternative tools for more specific enumeration of pathogenic Vv and Vp. Improvement of molecular methods can tighten better predictive models that are potentially important for mitigation in more controlled environments such as aquaculture.

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Response mechanism of Vibrio parahaemolyticus at high pressure revealed by transcriptomic analysis

Cited by 3 publications

References 72 publications

Insight into the adaptation mechanisms of high hydrostatic pressure in physiology and metabolism of hadal fungi from the deepest ocean sediment

Insight into the adaptation mechanisms of high hydrostatic pressure in physiology and metabolism of hadal fungi from the deepest ocean sediment

Response of Salmonella enterica serovar Typhimurium to alginate oligosaccharides fermented with fecal inoculum: integrated transcriptomic and metabolomic analyses

Tools to Enumerate and Predict Distribution Patterns of Environmental Vibrio vulnificus and Vibrio parahaemolyticus

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