Response mechanism of gut microbiome and metabolism of European seabass (Dicentrarchus labrax) to temperature stress

Liu, Yanyun; Cheng, Jianxin; Xia, Yuqing; Li, Xiaohao; Liu, Ying; Liu, Pengfei

doi:10.1016/j.scitotenv.2021.151786

Cited by 28 publications

(17 citation statements)

References 74 publications

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“…cathayensis gut microbiota were related to metabolism, followed by organismal systems, cellular processes pathways, human diseases pathways, genetic information processing pathways, and environmental information processing pathways ( Figure 4 ). The intestines are essential organs involved in the metabolism of nutrients ( Liu et al, 2022 ). The results suggest that Cd exposure may alter gut microbial function and host metabolism.…”

Section: Discussionmentioning

confidence: 99%

Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis

Jiang

et al. 2022

Front. Microbiol.

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As one of the most environmentally toxic heavy metals, cadmium (Cd) has attracted the attention of researchers globally. In particular, Guangxi, a province in southwestern China, has been subjected to severe Cd pollution due to geogenic processes and anthropogenic activities. Cd can be accumulated in aquatic animals and transferred to the human body through the food chain, with potential health risks. The aim of the present study was to explore the effects of waterborne Cd exposure (0.5 mg/L and 1.5 mg/L) on the intestinal microbiota of mudsnail, Cipangopaludina cathayensis, which is favored by farmers and consumers in Guangxi. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 segment of the bacterial 16S rRNA gene. Our results indicated that C. cathayensis could tolerate low Cd (0.5 mg/L) stress, while Cd exposure at high doses (1.5 mg/L) exerted considerable effects on microbiota composition. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were the dominant phyla in the mudsnail gut microbiota. The relative abundances of Bacteroidetes increased significantly under high Cd exposure (H14) (p < 0.01), with no significant change in the low Cd exposure (L14) treatment. The dominant genera with significant differences in relative abundance were Pseudomonas, Cloacibacterium, Acinetobacter, Dechloromonas, and Rhodobacter. In addition, Cd exposure could significantly alter the pathways associated with metabolism, cellular processes, environmental information processing, genetic information processing, human diseases, and organismal systems. Notably, compared to the L14 treatment, some disease-related pathways were enriched, while some xenobiotic and organic compound biodegradation and metabolism pathways were significantly inhibited in the H14 group. Overall, Cd exposure profoundly influenced community structure and function of gut microbiota, which may in turn influence C. cathayensis gut homeostasis and health.

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Section: Discussionmentioning

confidence: 99%

Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis

Jiang

et al. 2022

Front. Microbiol.

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Section: Discussionmentioning

confidence: 99%

“…Studies have shown that Firmicutes plays an important role in maintaining the gut homeostasis [ 53 ] and actively responds to temperature changes [ 54 ]. Notably, in the present study, the relative abundance of Firmicutes increased significantly in T1 and the decreased in T2, suggesting that thermal stress affects the gut homeostasis of C. cathayensis [ 54 ]. In addition, Firmicutes is known to be positively correlated with intestinal nutrient metabolism [ 32 , 46 ], and a reduction in Firmicutes abundance may hinder intestinal nutrient absorption [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…The functional prediction analysis showed that most genes of the C. cathayensis gut microbiota were related to metabolism. Gut microbiota contributes to host nutrition, absorption, and metabolism [ 54 ]; thus, thermal stress may affect C. cathayensis metabolism [ 54 ]. Indeed, in the present study, some carbohydrate metabolism pathways were found to be enriched in the T1 group but decreased in the T2 group compared to the T1 group.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of Gut Microbiome in the Mud Snail Cipangopaludina cathayensis in Response to High-Temperature Stress

Cheng

Liu

et al. 2022

Animals

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The mud snail Cipangopaludina cathayensis is a widely distributed species in China. Particularly in Guangxi province, mud snail farming contributes significantly to the economic development. However, global warming in recent decades poses a serious threat to global aquaculture production. The rising water temperature is harmful to aquatic animals. The present study explored the effects of high temperature on the intestinal microbiota of C. cathayensis. Snail intestinal samples were collected from the control and high-temperature groups on days 3 and 7 to determine the gut microbiota composition and diversity. Gut bacterial community composition was investigated using high-throughput sequencing of the V3–V4 region of bacterial 16S rRNA genes. Our results suggested that thermal stress altered the gut microbiome structure of C. cathayensis. At the phylum level, Proteobacteria, Bacteroidetes, and Firmicutes were dominant in C. cathayensis gut microbiota. The T2 treatment (32 ± 1 °C, day 7) significantly decreased the relative abundance of Firmicutes, Actinobacteria, and Deinococcus-Thermus. In T2, the abundance of several genera of putatively beneficial bacteria (Pseudomonas, Aeromonas, Rhodobacter, and Bacteroides) decreased, whereas the abundance of Halomonas—a pathogenic bacterial genus—increased. The functional prediction results indicated that T2 treatment inhibited some carbohydrate metabolism pathways and induced certain disease-related pathways (e.g., those related to systemic lupus erythematosus, Vibrio cholerae infection, hypertrophic cardiomyopathy, and shigellosis). Thus, high temperature profoundly affected the community structure and function of C. cathayensis gut microbiota. The results provide insights into the mechanisms associated with response of C. cathayensis intestinal microbiota to global warming.

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“…Global warming increases the average temperature in summer, and the probability of extremely high temperature, which will make cold-water sh face more frequent heat stress. Many studies have documented the signi cant effects of heatwave on Cold-water shes' immunity, growth metabolism and behavior [23,[28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis reveals the high temperature-induced damage is a significant factor affecting the osmotic function of gill tissue in cold-water fish

Yang

Lin

et al. 2022

Preprint

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Background Maintaining osmotic balance plays an important role in the normal operation and survival of cold-water fish. High water temperature has been proven to reduce the activity of NA+-K+-ATPase in the gill. However, the mechanism of megatemperature affect gill osmoregulation has not been fully elucidated. Results In this study, A.baerii, as a kind of iconic cold-water fish, was used to analyze histological alterations, plasma ion content, and transcriptional responses of gill of A.baerii, subjected to 20℃, 24℃and 28℃. We found that ROS levels in gill tissue increased and the gill damage deteriorated along with the temperature rising. Moreover, plasma Cl− levels at 28℃ were much lower than those in the other two groups, and there were no significant differences in Na+ and K+ ions among all trials. At the same time, the transcriptome data indicated that there were significant changes in injury-related pathways and genes at 24℃and 28℃. Meanwhile, GO enrichment analysis showed a large number of genes related to ion transport function changed with temperature increasing during cell processes. We discovered that as temperature increased, the expression of genes that regulate ion outflow (OCLN) and active ion absorption (AE2, NHE3, CLC-2) increased, while NKA-a expression decreased. Furthermore, the changes in osmotic regulation genes and the degree of tissue damage at 28℃ were significantly higher than those in the other two groups, indicating that the degree of heat stress-induced injury affects the osmotic regulation function of gill tissue. Conclusions this study reveals for the first time that the tissue damage caused by high temperature affects the function and the response mechanism of the osmotic regulation in gill of cold-water fish. It provides reference data for the study on the physiological function of cold-water fish under global warming.

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Response mechanism of gut microbiome and metabolism of European seabass (Dicentrarchus labrax) to temperature stress

Cited by 28 publications

References 74 publications

Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis

Effects of cadmium exposure on intestinal microflora of Cipangopaludina cathayensis

Characterization of Gut Microbiome in the Mud Snail Cipangopaludina cathayensis in Response to High-Temperature Stress

Transcriptome analysis reveals the high temperature-induced damage is a significant factor affecting the osmotic function of gill tissue in cold-water fish

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