Response characteristics of primary periodontal mechanoreceptive neurons simultaneously recorded in the trigeminal ganglion and mesencephalic trigeminal nucleus of the rat in response to trapezoidal and rectangular mechanical stimulation of a single tooth

Abstract

Periodontal mechanoreceptors are dually innervated by primary neurons whose cell bodies exist in the trigeminal ganglion (TG) and the mesencephalic trigeminal nucleus (MTN) . We obtained simultaneous recordings of singleunit responses of primary periodontal mechanoreceptive neurons located in the TG and MTN of the same animal to controlled experimental forces applied to a single tooth. Simultaneous singleunit recordings of electrophysiologically identified primary periodontal mechanoreceptive neurons in a rat's TG and MTN were performed while isosceles trapezoidal or rectangular mechanical stimuli were being applied in either the linguolabial or the labiolingual direction to the ipsilateral maxillary incisor. Fiftyfour single units were recorded in 27 rats and classified as rapidly adapting (RA) or slowly adapting (SA) periodontal neurons on the basis of their responses to a decisive rectangular mechanical stimulus applied in the linguolabial direction. Significantly more RA neurons existed in the MTN than the TG (P < 0.05) . The response characteristics of the SA and RA periodontal neurons were quite similar between the TG and MTN. The trapezoidal and rectangular stimuli applied in the linguolabial direction elicited a vigorous sustained spike discharge in SA periodontal neurons in both the TG and MTN, whereas both stimuli applied in the opposite (labiolingual) direction did not elicit any SA responses. Rectangular stimuli applied in both directions elicited highthreshold ON -OFF spike responses in the RA periodontal neurons in both the TG and MTN. We also found an unreported bursttype periodontal neuron; its responsiveness was characterized by a brief repetitive