Response and tolerance of yeast to changing environmental stress during ethanol fermentation

Delta-Integration of Single Gene Shapes the Whole Metabolomic Short-Term Response to Ethanol of Recombinant Saccharomyces cerevisiae Strains

“…Metabolomic analysis based on LC/MS identified a total of 143 metabolites with known structures ( Supplementary Table S1). This is the first report describing the metabolites produced by resting S. cerevisiae cells as a short-term ethanol response, whereas the metabolomic insights reported to date on ethanol stress in S. cerevisiae were applied only on cells growing in the presence of ethanol [23,[25][26][27].…”

“…The yeast cell reacts to ethanol by quickly reprogramming many cellular activities to promote survival during the stress condition and protect important cell components, which stimulate the normal activity of the cell. The ethanol stress responses are multifactorial and comprise various features such as signalling, gene level control and accumulation of the needed protectants [23]. Although profuse efforts have been spent at genetic, transcriptional and metabolomic levels, the precise mechanism of ethanol tolerance is not completely revealed [23,24] and a more holistic and in-depth understanding of ethanol tolerance in S. cerevisiae is crucial to support novel yeast metabolic engineering methodologies and improve ethanol production.…”

“…The ethanol stress responses are multifactorial and comprise various features such as signalling, gene level control and accumulation of the needed protectants [23]. Although profuse efforts have been spent at genetic, transcriptional and metabolomic levels, the precise mechanism of ethanol tolerance is not completely revealed [23,24] and a more holistic and in-depth understanding of ethanol tolerance in S. cerevisiae is crucial to support novel yeast metabolic engineering methodologies and improve ethanol production. Metabolomics has been gradually applied in investigating the cellular stress responses to ethanol [25][26][27].…”

Identificação e quantificação da expressão dos genes envolvidos no transporte de xilose na levedura >i<Candida guilliermondii>/i< FTI 20037

“…Extremamente adaptável, a membrana se molda de acordo com o momento atual da célula, sendo susceptível a características intra e extracelulares que agem em constante equilíbrio a fim de garantir a sobrevivência. As membranas devem suportar flutuações de temperatura, osmolaridade, produtos químicos tóxicos, a presença de radiações e longos períodos de privação de nutrientes, bem como a dessecação das células (SAINI et al, 2018).…”

Alternativas de hidrólise enzimática e fermentação em reatores de coluna para aplicação em biorrefinarias integradas de primeira e segunda geração empregando bagaço de cana-de-açúcar: dos experimentos em laboratório à avaliação de viabilidade econômica

“…As medidas de absorbância foram correlacionadas com a concentração celular (g/L) mediante uma curva de calibração previamente determinada. (EDGARDO et al, 2008;SAINI et al, 2018;SIQUEIRA JUNIOR et al, 2015). Na aclimatização progressiva dos microrganismos, a levedura Scheffersomyces stipitis não cresceu na temperatura de 42 º C, sendo que essa temperatura foi letal para a levedura.…”