Response and tolerance of root border cells to aluminum toxicity in soybean seedlings

Cai, Ming; Wang, Fangmei; Li, Rongfeng; Zhang, Shuna; Wang, Ning; Gen-di, XU

doi:10.1016/j.jinorgbio.2011.04.004

Cited by 40 publications

(18 citation statements)

References 39 publications

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“…Root border cells were sensitive to Al toxicity, which induced at loss of cell viability (Yu et al, 2006; Cai et al, 2011), that was mitigated by abundant B supply ( Figure 1A ). Previous study showed that B deficiency enhanced Al sensitivity in common beans (Stass et al, 2007) whereas B pretreatment alleviated Al toxicity to intact root or plants of cucumber and pea (Corrales et al, 2008; Yu et al, 2009b).…”

Section: Discussionmentioning

confidence: 99%

Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Liu

Fang

et al. 2017

Front. Plant Sci.

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Aluminum (Al) toxicity is the primary factor limiting crop growth in acidic soils. Boron (B) alleviates Al toxicity in plants, which is mainly considered to be due to the formation of Rhamnogalacturonan II-B (RGII-B) complexes, which helps to stabilize the cytoskeleton. It is unclear yet whether this is due to the increasing of net negative charges and/or further mechanisms. Kinetics of Al accumulation and adsorption were investigated using entire cells, cell wall and pectin of root border cells (RBCs) of pea (Pisum sativum), to reveal the mechanism of B in interacting with alkali-soluble and chelator-soluble pectin for an increased Al tolerance in RBCs. The results show that B could rescue RBCs from Al-induced cell death by accumulating more Al in the cell wall, predominately in alkali-soluble pectin. Boron also promotes Al3+ adsorption and inhibits Al3+ desorption from alkali-soluble pectin. Thus, more Al3+ is immobilized within the alkali-soluble pectin fraction and less in the chelator-soluble pectin, rendering Al3+ less mobile. Boron induces an increase of RG-II (KDO,2-keto-3-deoxyoctonic acid) content for forming more borate-RGII complexes, and the decrease of pectin methyl-esterification, thus creates more negative charges to immobilize Al3+ in cell wall pectin. The study provides evidence that abundant B supply enhances the immobilization of Al in alkali-soluble pectin, thus most likely reducing the entry of Al3+ into the symplast from the surroundings.

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Section: Discussionmentioning

confidence: 99%

Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Liu

Fang

et al. 2017

Front. Plant Sci.

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“…3, 5, and 6). Interestingly, root border cells have been suggested to be involved in tolerance to aluminum (Al), with the physical removal of border cells from the root increasing the toxic effects of Al (Cai et al, 2011). For Al, it is thought that the border cells protect roots by increasing the production of mucigel (which binds Al strongly and reduces entry into the root).…”

Section: Resultsmentioning

confidence: 99%

Examination of the Distribution of Arsenic in Hydrated and Fresh Cowpea Roots Using Two- and Three-Dimensional Techniques

et al. 2012

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Arsenic (As) is considered to be the environmental contaminant of greatest concern due to its potential accumulation in the food chain and in humans. Using novel synchrotron-based x-ray fluorescence techniques (including sequential computed tomography), short-term solution culture studies were used to examine the spatial distribution of As in hydrated and fresh roots of cowpea (Vigna unguiculata 'Red Caloona') seedlings exposed to 4 or 20 mm arsenate [As(V)] or 4 or 20 mm arsenite. For plants exposed to As(V), the highest concentrations were observed internally at the root apex (meristem), with As also accumulating in the root border cells and at the endodermis. When exposed to arsenite, the endodermis was again a site of accumulation, although no As was observed in border cells. For As(V), subsequent transfer of seedlings to an As-free solution resulted in a decrease in tissue As concentrations, but growth did not improve. These data suggest that, under our experimental conditions, the accumulation of As causes permanent damage to the meristem. In addition, we suggest that root border cells possibly contribute to the plant's ability to tolerate excess As(V) by accumulating high levels of As and limiting its movement into the root.

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“…The metal avoidance in plants such as A. rubrum is associated with morphological changes at the root system and likely involves auxins (Cai et al, 2011;Khare et al, 2017;Liu et al, 2011;Overvoorde, Fukaki, & Beeckman, 2010;Potters et al, 2007;Vitti et al, 2014). Plants also limit metal assimilation by the roots by secreting a number of substances such as organic acids, and substances in root extracellular matrix such as sugars, phenols, amino acids, and polysaccharides (Cai et al, 2011;Guo, Liang, & Zhu, 2009;Jutsz & Gnida, 2015). The mechanism used by A. rubrum to avoid nickel is unclear, but considering that the nickel treatment was conducted in a controlled environment using a sterilized sand / soil mix, it is likely that the avoidance mechanism is in situ.…”

Section: Gene Expression and Ontology Analysesmentioning

confidence: 99%

Differential levels of gene expression and molecular mechanisms between red maple (Acer rubrum) genotypes resistant and susceptible to nickel toxicity revealed by transcriptome analysis

Nkongolo

Theriault

Paul

2018

Ecology and Evolution

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Knowledge of regulation of genes associated with metal resistance in higher plants is very limited. Many plant species have developed different genetic mechanisms and metabolic pathways to cope with metal toxicity. The main objectives of this study were to 1) assess gene expression dynamics of A. rubrum in response to nickel (Ni) stress and 2) describe gene function based on ontology. Certified A. rubrum genotypes were treated with 1,600 mg of Ni per 1 Kg of soil corresponding to a soil total nickel content in a metal‐contaminated region in Ontario, Canada. Nickel resistant and susceptible genotypes were selected and used for transcriptome analysis. Overall, 223,610,443 bases were generated. Trinity reads were assembled to trinity transcripts. The transcripts were mapped to protein sequences and after quality controls and appropriate trimmings, 66,783 annotated transcripts were selected as expressed among the libraries. The study reveals that nickel treatment at a high dose of 1,600 mg/kg triggers regulation of several genes. When nickel‐resistant genotypes were compared to water controls, 6,263 genes were upregulated and 3,142 were downregulated. These values were 3,308 and 2,176, respectively, when susceptible genotypes were compared to water control. The coping mechanism of A. rubrum to Ni toxicity was elucidated. Upregulation of genes associated with transport in cytosol was prevalent in resistant genotypes compared to controls while upregulation of genes associated with translation in the ribosome was higher in susceptible genotypes when compared to water. The analysis revealed no major gene associated with Ni resistance in A. rubrum. Overall, the results of this study suggest that the genetic mechanism controlling the resistance of this species to nickel is controlled by genes with limited expression. The subtle differences between resistant and susceptible genotypes in gene regulation were detected using water‐treated genotypes as references.

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Response and tolerance of root border cells to aluminum toxicity in soybean seedlings

Cited by 40 publications

References 39 publications

Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Examination of the Distribution of Arsenic in Hydrated and Fresh Cowpea Roots Using Two- and Three-Dimensional Techniques

Differential levels of gene expression and molecular mechanisms between red maple (Acer rubrum) genotypes resistant and susceptible to nickel toxicity revealed by transcriptome analysis

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