The environmentally friendly fertilizer is necessary for vegetable production that are safe for consumption and maintain soil fertility. This study aims to determine the effect of the composition of urea fertilizer and Mexican sunflower compost (MSC) on the production and quality of various basil accessions (Ocimum basilicum L.). This study uses a factorial completely randomized design, namely basil accession (Gegerbitung, Kadudampit 1, Kadudampit 2, Kemang, Ciaruteun, Cijujug, and Gasol) and the composition of N fertilizer (100% N-urea, 100% N-MSC, 75% N-urea + 25% N-MSC, 50% N-urea + 50% N-MSC, 25% N-urea + 75% N-MSC, and 0% N-urea + 0% N-MSC). The results showed that plants Kadudampit 2 and Gasol have growth and production better than other accessions. Plants treated with 100% N-MSC produced growth and harvest wet and dry weight not significantly different from 100% N-urea. Nitrate and vitamin C content were not significantly different between accessions. The use of 100% MSC significantly reduces leaf nitrate content. The vitamin C content was not significantly different between plants that were fertilized with various compositions of N. The content of chlorophyll a, chlorophyll b, and carotenoids of Gasol and Ciaruteun accessions were the lowest compared to the other five accessions. The highest chlorophyll a, chlorophyll b content are found in plants with Urea, followed by those given a combination of Urea + MSC. The carotenoid content of plant treated with 100% Urea was not significantly different from those fertilized with 75% Urea + 25% MSC and 25% Urea + 75% MSC.