Respiratory Viral Coinfections Identified by a 10-Plex Real-Time Reverse-Transcription Polymerase Chain Reaction Assay in Patients Hospitalized With Severe Acute Respiratory Illness—South Africa, 2009–2010

Abstract

The data provide a better understanding of the viral etiology of hospitalized cases of pneumonia and demonstrate the usefulness of this multiplex assay in respiratory disease surveillance in South Africa.

Epidemiology of Acute Lower Respiratory Tract Infection in HIV-Exposed Uninfected Infants

“…Specimens were tested by multiplex realtime reverse-transcription PCR for 10 respiratory viruses including influenza A and B viruses, parainfluenza virus types 1, 2, and 3; respiratory syncytial virus (RSV); enterovirus; human metapneumovirus (hMPV); adenovirus; and human rhinovirus. 22 Streptococcus pneumoniae was identified by quantitative real-time PCR detecting the lytA gene from whole blood specimens. 23 …”

Estimating vaccine effectiveness in preventing laboratory‐confirmed influenza in outpatient settings in South Africa, 2015

“…Influenza A‐positive specimens were further subtyped by rRT‐PCR 7. Clinical, demographic and influenza vaccination data were collected from each patient at the time of specimen collection.…”

Human metapneumovirus-associated severe acute respiratory illness hospitalisation in HIV-infected and HIV-uninfected South African children and adults

“…24 Streptococcus pneumoniae was identified by lytA quantitative realtime PCR detection from whole blood specimens. 25 Determination of HIV infection status was based either on HIV testing undertaken at the discretion of the attending physician or through anonymised linked dried blood spot specimen testing (HIV PCR for patients <18 months and enzyme-linked immunosorbent assay (ELISA) for patients ≥18 months of age) as part of the study.…”