Blood (hematocrit, hemoglobin and red blood cell) and plasma (cortisol, glucose, lactic acid, Na + , Cl -, K + , osmolality, aspartate aminotransferase and alanine aminotransferase) factors in greenling, Hexagrammos otakii after MS-222 and lidocaine-HCl anaesthetic were determined. Greenling (mean body length: 25.8 ± 1.6 cm, mean body weight: 194.5 ± 33.8 g) were exposed to concentrations of 125 ppm MS-222 and 800 ppm lidocaine-HCl at 18°C. Blood was collected from ten fish after anesthesia 0.5, 1, 3, 6 and 24 hours, respectively. After anesthesia, the concentrations of hematocrit, hemoglobin, red blood cell, cortisol, glucose, lactic acid, osmolality, Na + , K + , Cl -, aspartate aminotransferase and alanine aminotransferase were determined. Control and experimental group displayed significant increases in plasma cortisol concentrations from their respective prestress levels at 0.5 hour after anaesthetic (P<0.05). Concentrations of glucose were increased from 22.3 ng/ mL to 28.5 ng/mL (0.5 hour), 31.0 ng/mL (1 hour) in experimental group, respectively. Lactic acid concentrations for experimental group were lower than those for control group at 0.5, 1 and 3 hours. In other items, control and experimental group did not show significant differences in this before and after anaesthetic. The results indicate that lidocaine-HCl and MS-222 can be used as suitable anaesthetic for the greenling.