Respiratory nitrate reductase from haloarchaeon Haloferax mediterranei: biochemical and genetic analysis

Lledó, Belén; Martínez‐Espinosa, Rosa María; Marhuenda-Egea, Frutos C.; Bonete, Marı́a José

doi:10.1016/j.bbagen.2004.05.007

Cited by 67 publications

(107 citation statements)

References 36 publications

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“…The absorption spectrum of the Hfx. mediterranei Nar showed a broad band around 400 to 415 nm indicating that this enzyme has Fe-S clusters as other Nar purified from denitrifying microorganisms (Lledó et al, 2004).…”

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

“…mediterranei two different non-assimilatory nitrate reductases have been purified and characterised: a dissimilatory nitrate reductase described by Alvarez-Ossorio, Muriana, de la Rosa & Relimpio 1992, and Nar characterised by Lledó et al 2004. The expression of the first one was induced not only by means of the presence of nitrate as nitrogen source but also by the switch to anaerobic conditions.…”

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

“…The electron donor studies revealed that methyl and benzyl viologen are the best for this enzyme while FMA o FAD were ineffective electron donors (Alvarez-Ossorio et al, 1992). According to its molecular mass and enzymatic properties, Lledó et al (2004) proposed that the enzyme purified by Alvarez-Ossorio allows the dissipation of reducing power for redox balancing. The Hfx.…”

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

“…Later, the analysis of N-terminal region of the archaeal nitrate reductases revealed the conservation of a twin-arginine motif (Martinez-Espinosa et al, 2007). This data underlines the fact that NarG protein could be strongly attached to the membrane fraction and requires detergent solubilisation to release it (Lledó et al 2004). In order to study the location of the NarG, amino acid sequence analysis and bioinformatic studies were carried out with Hfx.…”

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

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Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Bacterial Electron Transport Systems and Their Regulation

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A number of species of Haloferax genus (halophilic Archaea) are able to grow micro aerobically or even anaerobically using different alternative electron acceptors such as fumarate, nitrate, chlorate, dimethyl sulphoxide, sulphide and/or trimethylamine. This metabolic capability is also shown by other species of the Halobacteriaceae and Haloferacaceae families (Archaea domain) and it has been mainly tested by physiological studies where cells growth is observed under anaerobic conditions in the presence of the mentioned compounds. This work summarises the main reported features on anaerobic metabolism in the Haloferax, one of the better described haloarchaeal genus with significant potential uses in Biotechnology and Bioremediation. Special attention has been paid to denitrification, also called nitrate respiration. This pathway has been studied so far from KEY WORDSHaloarchaea, denitrification, anaerobic metabolism, Nox emissions, bioremediation.

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Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

Section: Respiratory Nitrate Reductases In Haloferax Genusmentioning

confidence: 99%

See 2 more Smart Citations

Anaerobic Metabolism in Haloferax Genus

Torregrosa-Crespo

Martínez‐Espinosa

Esclapez

et al. 2016

Advances in Bacterial Electron Transport Systems and Their Regulation

Self Cite

View full text Add to dashboard Cite

show abstract

“…It has been demonstrated that Hfx. mediterranei grows under anaerobic conditions using nitrate as terminal electron acceptor [8], and we observed the induction of respiratory nitrate reductase (Nar) activity in these cultures. These results agree with other studies in which nitratereducing and denitrifying activities are induced under oxygen-limiting conditions only in the presence of nitrate [17].…”

Section: Introductionmentioning

confidence: 87%

A family of models to study the growth of Haloferax mediterranei in different conditions

Villacampa¹,

García-Alonso²,

Reyes³

et al. 2007

WIT Transactions on Ecology and the Environment, Vol 106

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Haloferax mediterranei is a denitrifying halophilic archaeon able to grow with nitrite as the sole nitrogen source for growth in an assimilatory process under aerobic conditions. This haloarchaeon can also reduce nitrite in a respiratory process, where nitrite is the electron acceptor when oxygen conditions are limited. Due to this capability, Haloferax mediterranei could be applied in salted water bioremediation processes with the purpose of repairing the damage caused by the excessive use of fertilizers in agricultural activities.In this paper a family of different mathematical models has been generated to allow the study and the prediction of Hfx. mediterranei growth in high salt media with different nitrite concentrations. The relation between the growth and some variables are studied, for example nitrite concentration (N), oxygen concentration (O) and the time of growth (NH). This approach will allow us to analyse future Hfx. mediterranei uses as agent for bioremediation processes.

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The Membrane‐Bound Nitrate Reductase A from Escherichia Coli: Nar GHI

Bertero

2004

Handbook of Metalloproteins

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The bacterium Escherichia coli is able to survive in a wide range of adverse environmental conditions. When oxygen is limited and nitrate is present, E. coli synthesizes a specific electron transfer chain to produce energy for its own survival. The chain is constituted by two membrane‐bound enzymes: the initial formate dehydrogenase FdnGHI and the terminal nitrate reductase NarGHI. Electrons are transferred from the formate oxidation site to the nitrate reduction site with a net translocation of protons across the cytoplasmic membrane. In the recent years, crystallographic studies have shed light on the structure and function of the two enzymes revealing a clear picture of the nitrate electron transfer chain at the atomic level. The present article focuses on the advances obtained on NarGHI, its function and architecture, and the eight redox cofactors that form like an electrical wire through all the enzymes, starting from the electron acceptor site to the catalytic center.

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Respiratory nitrate reductase from haloarchaeon Haloferax mediterranei: biochemical and genetic analysis

Cited by 67 publications

References 36 publications

Anaerobic Metabolism in Haloferax Genus

Anaerobic Metabolism in Haloferax Genus

A family of models to study the growth of Haloferax mediterranei in different conditions

The Membrane‐Bound Nitrate Reductase A from Escherichia Coli: Nar GHI

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