Respiratory Mitochondrial Efficiency and DNA Oxidation in Human Sperm after In Vitro Myo-Inositol Treatment

Governini, Laura; Ponchia, Rosetta; Artini, Paolo Giovanni; Casarosa, Elena; Marzi, Ilaria; Capaldo, Angela; Luddi, Alice; Piomboni, Paola

doi:10.3390/jcm9061638

Cited by 21 publications

(24 citation statements)

References 45 publications

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“…For instance, increased ROS production causes DNA fragmentation or lipid peroxidation, which compromises the membrane and impairs mitochondrial activity [ 53 , 54 ]. In fact, antioxidants have been proposed as adjuvants for sperm culture medium, being beneficial for spermatozoa viability and motility maintenance [ 55 , 56 , 57 ]. Since increased ROS production can lead to oxidative stress-induced damage, we also analyzed lipid peroxidation, protein oxidation, and protein nitration.…”

Section: Discussionmentioning

confidence: 99%

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

et al. 2020

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Spermatozoa capacitation is a complex process that requires specific ionic and energetic conditions to support biochemical alterations leading to motility hyperactivation. However, human sperm capacitation is still poorly understood. Herein, we studied the effects of glucose on human sperm capacitation. Healthy men seminal samples (n = 55) were submitted to a density gradient centrifugation and incubated in capacitating conditions in the absence or presence of increasing glucose concentrations (0, 5.5, 11, and 22 mM). Viability and total motility were accessed. Phosphotyrosine levels were measured. Mitochondrial activity and endogenous ROS production were evaluated. Oxidative stress-induced damage was analyzed. Culture media was collected and analyzed by 1H-NMR. Our results show that glucose is essential for human sperm capacitation and motility. Notably, we observed that mitochondrial activity increased even in the absence of glucose. This increased mitochondrial activity was followed by a ROS overproduction, although no oxidative stress-induced damage was detected. Our results show that glucose is essential for capacitation but mitochondrial activation is independent from its stimuli. ROS overproduction may take part on a finely regulated signaling pathway that modulates or even activates capacitation. Taken together, our results constitute a paradigm shift on human sperm capacitation physiology.

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Section: Discussionmentioning

confidence: 99%

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

et al. 2020

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“…Therefore, researchers decided to evaluate 8-OHdG, one of the first products of oxidative damage to DNA. The samples treated with MI displayed reduced levels of 8-OHdG [153]. Therefore, MI can improve the sperm parameters related to the quality and in vitro fertilization process.…”

Section: Mi: In Vitro Studiesmentioning

confidence: 91%

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

et al. 2021

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Infertility is defined as a couple’s inability to conceive after at least one year of regular unprotected intercourse. This condition has become a global health problem affecting approximately 187 million couples worldwide and about half of the cases are attributable to male factors. Oxidative stress is a common reason for several conditions associated with male infertility. High levels of reactive oxygen species (ROS) impair sperm quality by decreasing motility and increasing the oxidation of DNA, of protein and of lipids. Multi-antioxidant supplementation is considered effective for male fertility parameters due to the synergistic effects of antioxidants. Most of them act by decreasing ROS concentration, thus improving sperm quality. In addition, other natural molecules, myo-inositol (MI) and d-chiro–inositol (DCI), ameliorate sperm quality. In sperm cells, MI is involved in many transduction mechanisms that regulate cytoplasmic calcium levels, capacitation and mitochondrial function. On the other hand, DCI is involved in the downregulation of steroidogenic enzyme aromatase, which produces testosterone. In this review, we analyze the processes involving oxidative stress in male fertility and the mechanisms of action of different molecules.

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“…In addition, it has been determined that inositol increases mitochondrial calcium levels, improves sperm mitochondrial function with an antioxidative effect and prevents apoptosis [ 32 ]. Governin et al [ 54 ] demonstrated the ability to prevent oxidation damage in DNA with 20 mg/mL MI, with a significant decrease in the level of 8-OHdG in sperm. It has been shown in different studies that the antioxidant properties of inositol on sperm quality, oxidant and antioxidant levels and DNA damage, when added before cryo-storage, differ according to the type of animal used.…”

Section: Introductionmentioning

confidence: 99%

Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm

Doğu

Şahinöz

Aral³

et al. 2021

Animals

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This study was based on the determination of post-thaw semen quality, oxidant–antioxidant status and DNA-damage status of frozen Mesopotamian catfish semen. To this end, the sperm was frozen in diluent containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly increased the spermatozoa motility rate and duration significantly (p < 0.05). High doses of inositol increased antioxidants and decreased oxidants. In addition, lower intracellular DNA damage and percentage of apoptotic spermatozoa occurred in high doses of inositol (p < 0.05).Abstract: In this study, the effects of supplemented inositol on sperm extenders were examined on the spermatozoa motility rate and duration, total antioxidant and oxidant status, apoptotic spermatozoa and DNA damage, during the sperm post-thaw process of Mesopotamian Catfish (Silurus triostegus, H. 1843). The semen was frozen in diluents containing different inositol concentrations (5, 10, 20 and 40 mg). Increasing levels of inositol linearly improved the spermatozoa motility rate and duration significantly (p < 0.05). MDA and TOS were linearly decreased, however, TAS and GSH linearly increased (p < 0.05). The increasing inositol levels resulted in a linear and quadratic decrease in DNA damage in the comet assay, 8-hydroxydeoxyguanosine and the determined percentage of apoptotic spermatozoa (p < 0.05). These results suggest that there are many positive effects of the use of supplemental inositol on enhancing sperm cryopreservation efficiency in Silurus triostegus.

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Respiratory Mitochondrial Efficiency and DNA Oxidation in Human Sperm after In Vitro Myo-Inositol Treatment

Cited by 21 publications

References 45 publications

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

Mitochondrial Activation and Reactive Oxygen-Species Overproduction during Sperm Capacitation are Independent of Glucose Stimuli

Oxidative Stress and Male Fertility: Role of Antioxidants and Inositols

Effects of Inositol Supplementation in Sperm Extender on the Quality of Cryopreserved Mesopotamian Catfish (Silurus triostegus, H. 1843) Sperm

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