Due to the recent situation, especially since the beginning of the spread of COVID‐19, work in administrative or service offices under conditions of social divergence and the working environment has become conditional on the ventilation system; thus, enhancing ventilation's function to minimize the spread of infections, and impurities to larger areas or limiting its transition from one employee to another who work in the same place for long hours. A nonisothermal office room, designed with a mixing ventilation system has been simulated by using AIRPAK3.0.16 software, and RNG k–ε $\varepsilon $ as a turbulence model, where the adoption of partitions in such rooms was studied and its effect on thermal employee's comfort and quality of fresh air, by monitoring the air diffusion performance index, prediction of the mean vote, and percentage of predicted dissatisfied. The main findings were that partitions are seen as a technique of preventing and protecting against the spread of pollutants and illnesses since they prevent contaminants from reaching the breathing zones of the office's inhabitants. It was noted that the change in the height of the partition with an increase of 10% of the room's height as a gap, gives a more acceptance value of the heat removal efficiency and ventilation rate.