Respiratory granules of heart muscle

Cleland, K. W.; Slater, E.C.

doi:10.1042/bj0530547

Cited by 671 publications

(122 citation statements)

References 28 publications

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“…Mitochondria were isolated as described by Myers and Slater [8], suspended in the same medium without EGTA and kept at 0°C. Mitochondrial protein was determined by a biuret method [9]. To measure pyruvate uptake, freshly isolated mitochondria {approx.…”

Section: Methodsmentioning

confidence: 99%

Accumulation of pyruvate by isolated rat liver mitochondria

Vaartjes

Geelen

Bergh

1979

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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Section: Methodsmentioning

confidence: 99%

Accumulation of pyruvate by isolated rat liver mitochondria

Vaartjes

Geelen

Bergh

1979

Biochimica et Biophysica Acta (BBA) - Bioenergetics

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“…The biuret method as modified by Cleland & Slater (1953) was used, as described by Gillham (1971), for the determination of protein in the first five stages of the enzyme purification. The protein present in the final two stages of the purification was measured bytheextinction at 280nm: E"' 1.0/2.9mg of protein in 3ml (calibrated by comparison with biuret samples).…”

Section: Animalsmentioning

confidence: 99%

The mechanism of the reaction between glutathione and 1-menaphthyl sulphate catalysed by a glutathione S-transferase from rat liver

Gillham

1973

Biochemical Journal

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1. The glutathione S-transferase that catalyses the reaction of 1-menaphthyl (naphth-lylmethyl) sulphate with GSH was purified 76-fold from rat liver. 2. The properties of the purified enzyme were studied by gel filtration and isoelectric focusing. 3. The initialvelocity pattern in the absence of products and the product-inhibition pattern have been determined. These are consistent with an Ordered Bi Bi mechanism in which the GSH adds to the enzyme before 1-menaphthyl sulphate and the products are released in the order SO42-followed by S-(1-menaphthyl)glutathione. 4. Dead-end-inhibition studies with p-aminobenzoic acid, which has been shown to be competitive with GSH and noncompetitive with 1-menaphthyl sulphate, support the suggestion that an Ordered Bi Bi mechanism is operative. 5. Values were determined for some of the dissociation and Michaelis constants for the reaction of the substrates and products with the enzyme. 6. It appears that S{1-menaphthyl)glutathione activates the enzyme when the concentration of GSH is saturating and that of 1-menaphthyl sulphate is low (of the order of its Michaelis constant).

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“…Submitochondrial particles were prepared by the method of Gregg [4]. Protein was determined by the biuret method as described by Cleland and Slater [5]. All reactions were carried out in the medium containing 25 mM Tris-HCl buffer (pH 7.4), 50 mM sucrose, 5 mM MgClz ,2 mM EDTA and 15 mM KCl.…”

Section: Methodsmentioning

confidence: 99%